A Thermoelectrically Cooled Microtome Table and Knife

Rutherford, T. R.; Hardy, W. S.; Isherwood, P. A.

doi:10.3109/10520296409061227

Cited by 9 publications

(2 citation statements)

References 8 publications

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“…Apparatus for the preparation of frozen sections has been described by Brown and Dilly (1961), by Okamoto and Mizuno (1963) and by Rutherford et al (1964); however, I have found no report on the use of thermoelectric freezing for squash preparations. Apparatus for the preparation of frozen sections has been described by Brown and Dilly (1961), by Okamoto and Mizuno (1963) and by Rutherford et al (1964); however, I have found no report on the use of thermoelectric freezing for squash preparations.…”

Section: Thermoelectric Freezing For Removal Of Cover Glasses From Sqmentioning

confidence: 99%

Notes on Technic

1967

Stain Technology

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Recently in the Parasite Laboratory at Roanoke College it became necessary to work with digenetic trematodes fixed and stored in Gilson's fluid for more than a year. When starting with fresh parasites, Siddiqi and Cable (1 960) employed a sublimate-acetic fixative if immediate processing was possible. They washed the specimens, dehydrated to 70% ethanol and treated them with an alcoholic 1,KI solution to remove mercurial precipitates. Another technique for removing precipitates (Galigher and Kozloff 1964) requires dropwise addition of an I,KI solution to one of the dehydrating alcohols containing the specimens. Both these methods were quite inadequate for our purpose because much precipitate remained even after the worms had been strongly colored by iodine.We suspected that the concentration of iodine was too low in the customary procedures to react with all the precipitate which had accumulated during gross overfixation of more than a year in Gilson's fluid; therefore, we used an 1,KI solution which contained 5% iodine. The technique which restored nearly all of the overfixed specimens to usefulness for taxonomic studies is as follows: Procedure 1. Soak the specimens in 70% ethanol for about 48 hr, changing the fluid at 12-16 hr intervals.

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Section: Thermoelectric Freezing For Removal Of Cover Glasses From Sqmentioning

confidence: 99%

Notes on Technic

1967

Stain Technology

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“…With the advent of suitable thermoelectric modules, and their successful application to freezing microtomes (Okamoto & Mizuno, 1963;Rutherford et al, 1964), further development of cold microtomy was foreshadowed. During the past 3 years we have carried out a series of experiments, and these are briefly reported below.…”

Section: F U R T H E R Developmentsmentioning

confidence: 99%