A thermodynamic study on the interaction between magnesium ion and human growth hormone

Saboury, Ali Akbar; Atri, Maliheh Sadat; Sanati, Mohammad Hossein; Moosavi-Movahedi, Ali Akbar; Hakimelahi, G. H.; Sadeghi, Mehdi

doi:10.1002/bip.20386

Cited by 39 publications

(16 citation statements)

References 23 publications

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“…A thermodynamic study through ITC was performed which is a direct methodology of interpreting various thermodynamic parameters in various bio molecular interaction studies [37][38][39]. A reversed protocol was used throughout the experiment as the alkaloid has high heat of dilution [ Figure 5].…”

Section: Spectroscopic and Calorimetric Studiesmentioning

confidence: 99%

Therapeutic potential of harmaline, a novel alkaloid, against cervical cancer cells in vitro: Apoptotic induction and DNA interaction study

2018

J App Biol Biotech

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The study emphasizes the growth inhibitory effect of harmaline on HeLa (human cervical cancer) cell line and mode and mechanism of binding with CT DNA (calf thymus DNA). The results of cytotoxic study performed through MTT assay indicates that harmaline have concentration dependent growth inhibitory effect on HeLa cell line with GI 50 value of 28 µM. Furthermore, the alkaloid induced DNA damage and changes in mitochondrial membrane potential in the cell line. The alkaloid shows reactive oxygen species dependent cellular damage with significant arrest in G 2 /M population of the cell. Biophysical experiments further established the K i w value (product of cooperative binding affinity and the cooperative factor) of harmaline with CT DNA to be 5.60 × 10 5 M −1. Harmaline showed a progressive quenching of the fluorescence emission spectra. Circular dichroism study shows significant structural changes of DNAwith subsequent induction of optical activity in the bound achiral alkaloid molecule. The alkaloid stabilizes the DNA to 10°C. Intercalated state of harmaline inside DNA helix was shown by viscometric and ferrocyanide quenching. The results highlight the importance of alkaloid-DNA interaction for developing nucleic acid based therapeutic agents.

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Section: Spectroscopic and Calorimetric Studiesmentioning

confidence: 99%

Therapeutic potential of harmaline, a novel alkaloid, against cervical cancer cells in vitro: Apoptotic induction and DNA interaction study

2018

J App Biol Biotech

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“…The calorimetric data analysis using equation (16) is a graphical fitting model, which extensively has been applied to studies on the enzyme inhibition [32][33][34]50] and metal binding to proteins [8,[58][59][60][78][79]. These two proposed data analysis for isothermal titration calorimetry is expected useful for a set of binding sites, especially for enzyme inhibition study and measurement of inhibition constant.…”

Section: Ligand Binding In One Binding Site (1:1 Stochiometry)mentioning

confidence: 99%

“…If α is defined as the fraction of free binding sites on the biomacromolecule, M 0 is the total biomacromolecule concentration and L 0 is the total ligand concentration, then the free concentrations of monovalent molecule, [M], and ligand, [L], as well as the concentration of bound ligand, [ML], can be deduced as follow [56,58]:…”

Section: Ligand Binding In a Set Of Binding Sitesmentioning

confidence: 99%

A review on the ligand binding studies by isothermal titration calorimetry

Saboury

2006

JICS

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Thermodynamics of biomacromolecule ligand interaction is very important to understand the structure function relationship in proteins. One of the most powerful techniques useful to obtain additional information about the structure of proteins in biophysical chemistry field is isothermal titration calorimetry (ITC). An ITC experiment is a titration of a biomacromolecule solution by a solution containing a reactant (ligand) at constant temperature to obtain the exchanged heat of the reaction. The total concentration of ligand is the independent variable under experimental control. There are many reports on data analysis for ITC to find the number of binding sites (g), the equilibrium constant (K), the Gibbs free energy of binding process (ΔG), the enthalpy of binding (ΔH) and the entropy of binding (ΔS). Moreover, ITC gives information about the type of reaction, electrostatic and hydrophobic interactions, including determination of cooperativity characterization in binding process by calculating the Hill coefficient (n). A double reciprocal plot and a graphical fitting method are two simple methods used in the enzyme inhibition and metal binding to a protein. Determination of a binding isotherm needs more ITC experiments and more complex data analysis. Protein denaturation by ligand includes two processes of binding and denaturation so that ITC data analysis are more complex. However, the enthalpy of denaturation process obtained by ITC help to understand the fine structure of a protein.

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“…ITC gives invaluable information about biomacromolecule-ligand interactions. During the last two years we attempted to study the metal ion binding chemistry of different proteins [4][5][6][7][8][9][10][11][12]. They will change the conformational stability and form aggregates.…”

Section: Introductionmentioning

confidence: 99%

A New Approach for Titration Calorimetric Data Analysis on the Binding of Magnesium Ion with Myelin Basic Protein

2008

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The interaction of the myelin basic protein (MBP) from the bovine central nervous system with divalent magnesium ion was studied by isothermal titration calorimetry at 27°C in aqueous solution. A simple rapid method for determination of the dissociation binding constants for Mg 2+ -MBP interaction was introduced using the isothermal titration calometric data. The binding isotherm for Mg 2+ -MBP interaction is easily obtained by carrying out a titration calorimetric experiment using only one set of concentrations of MBP. There are two identical independent intrinsic association constants equal to 0.021 μmol·L −1 in the first-and second-binding sites, respectively.

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A thermodynamic study on the interaction between magnesium ion and human growth hormone

Cited by 39 publications

References 23 publications

Therapeutic potential of harmaline, a novel alkaloid, against cervical cancer cells in vitro: Apoptotic induction and DNA interaction study

Therapeutic potential of harmaline, a novel alkaloid, against cervical cancer cells in vitro: Apoptotic induction and DNA interaction study

A review on the ligand binding studies by isothermal titration calorimetry

A New Approach for Titration Calorimetric Data Analysis on the Binding of Magnesium Ion with Myelin Basic Protein

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