A therapeutic probe for detecting and inhibiting ONOO⁻ in senescent cells

Abstract

A new therapeutic probe for detecting and inhibiting ONOO− in senescent cells.

A ratiometric fluorescent probe revealing the abnormality of acetylated tau by visualizing polarity in Alzheimer's disease

“…[10][11][12][13][14][15][16][17] Particularly, ratiometric fluorescent probes can provide more accurate pathological information compared with turn-on/off fluorescent probes because it is not disturbed by probe concentration, photobleaching, autofluorescence and nonspecific background. [18][19][20] However, previously reported ratiometric or turn-on/off probes applied to AD were mainly for Ab plaques, 17,21,22 reactive oxygen species (ROS), [23][24][25][26] reactive sulfur species (RSS), 27,28 reactive nitrogen species (RNS) [29][30][31] Reactive carbonyl species (RCS), 32,33 metal ions [34][35][36] and adenosine triphosphate (ATP), 37 while few fluorescent probes were developed for polarity in AD-microenvironment. [38][39][40] Therefore, there is an urgent need to develop a ratiometric probe for monitoring polarity changes during AD.…”

Aptamer Conjugate-Based Ratiometric Fluorescent Probe for Precise Imaging of Therapy-Induced Cancer Senescence

“…Indeed, various activity-based imaging senoprobes have been developed and demonstrated great opportunities for sensing senescent cancer cells in a noninvasive, longitudinal manner. , They can be roughly divided into two types. The first, also the most widely investigated, type of senoprobes is designed in response to a single senescence-associated marker, such as enzymes, ions, and reactive species. − Nevertheless, such single-marker detection design typically suffers from low specificity because currently available markers are senescence-related but not specified. To address this issue, engineering dual-marker activated senoprobes has been emerging as an alternative strategy, in which imaging signals can be switched on only after sequential activation by two senescence-related markers, thereby offering enhanced imaging selectivity compared to the former strategy. − Intriguingly, we have recently found that such dual-marker recognition strategy could distinguish senescence-associated expression of markers from endogenous expression .…”