A theoretical study on the binding of O2, NO and CO to heme proteins

Blomberg, L. Mattias; Blomberg, Margareta R. A.; Siegbahn, Per E. M.

doi:10.1016/j.jinorgbio.2005.02.014

Cited by 98 publications

(143 citation statements)

References 49 publications

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“…Furthermore, RuCO, but not its respective compound RuCl 3 , possessed the ability to inhibit MMP-9 enzyme activity in a condition with or without cells. Previous studies indicated that CO reserved the ability to interact with several proteins including NADPH oxidase, iNOS, and cytochrome c reductase (40,44). This suggests that (a) metal component (Ru) is not involved in RuCO inhibition of tumor invasion and MMP-9 activation induced by TPA, (b) CO participates in HO-1 inhibition of TPA-induced tumor invasion of breast carcinoma cells via suppressing ERK/c-Jun/AP-1 activation, and (c) CO interaction with the MMP-9 protein may contribute to its inhibition on MMP-9 enzyme activity in vitro.…”

Section: Discussionmentioning

confidence: 99%

Heme oxygenase-1 inhibits breast cancer invasion via suppressing the expression of matrix metalloproteinase-9

Lin¹,

Shen

Hou

et al. 2008

Molecular Cancer Therapeutics

112

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In the present study, we investigated the antitumor effects of the invasiveness and migration of heme oxygenase 1 (HO-1) in human breast carcinoma cells. 12-O-tetradecanoylphorbol-13-acetate (TPA) -induced matrix metalloproteinase-9 (MMP-9) enzyme activity and gene expression at both protein and mRNA levels were examined in human breast carcinoma cells (MCF-7 and MDA-MB-231), and the addition of the MMP-9 inhibitor, SB3CT, significantly suppressed TPA-induced invasion and migration according to the in vitro Transwell assay. Elevation of HO-1 gene expression by ferric protoporphyrin IX inhibited TPA-induced invasion of MCF-7 cells, which was blocked by adding the heme oxygenase inhibitor, tin protoporphyrin IX, or transfection of cells with HO-1 short hairpin RNA. MCF-7 cells overexpressing HO-1 (MCF-7/HO-1) were established in the present study, and TPA-induced MMP-9 gene expression, tumor invasion, and colony formation were significantly reduced in MCF-7/HO-1 cells, compared with those in Neo-transfected cells. Activation of protein kinase CA/extracellular signalregulated kinases/AP-1 with stimulation of reactive oxygen species production was involved in TPA-induced invasion of MCF-7 cells, which was attenuated by HO-1 protein induced by ferric protoporphyrin IX or transfection of HO-1 expression vectors. Additionally, the addition of carbon monoxide, but not ferric ions, biliverdin, or bilirubin, inhibited TPA-induced invasion through suppressing MMP-9, extracellular signal-regulated kinases, and AP-1 activation stimulated by TPA. The beneficial role of HO-1 in blocking tumor invasion was first identified in this study.

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Section: Discussionmentioning

confidence: 99%

Heme oxygenase-1 inhibits breast cancer invasion via suppressing the expression of matrix metalloproteinase-9

Lin¹,

Shen

Hou

et al. 2008

Molecular Cancer Therapeutics

112

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“…[40][41][42] The fact that CO outcompetes the binding of O 2 allows our sensor to operate under ambient conditions.…”

Section: Author Manuscript Author Manuscriptmentioning

confidence: 99%

Bio‐Inspired Carbon Monoxide Sensors with Voltage‐Activated Sensitivity

Savagatrup

Schroeder

et al. 2017

Angewandte Chemie

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Correspondence to: Timothy M. Swager. + These authors contributed equally to this work.Supporting information and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.org/ 10.1002/anie.201707491. Conflict of interestThe authors declare the following competing financial interest(s): A patent has been filed on this technology. HHS Public Access Author Manuscript Author ManuscriptAuthor Manuscript Author ManuscriptCarbon monoxide (CO) outcompetes oxygen when binding to the iron center of hemeproteins, leading to a reduction in blood oxygen level and acute poisoning. Harvesting the strong specific interaction between CO and the iron porphyrin provides a highly selective and customizable sensor. We report the development of chemiresistive sensors with voltage-activated sensitivity for the detection of CO comprising iron porphyrin and functionalized single-walled carbon nanotubes (F-SWCNTs). Modulation of the gate voltage offers a predicted extra dimension for sensing. Specifically, the sensors show significant increase in sensitivity toward CO when negative gate voltage is applied. The dosimetric sensors are selective to ppm levels of CO and functional in air. UV/Vis spectroscopy, differential pulse voltammetry, and density functional theory reveal that the in situ reduction of Fe III to Fe II enhances the interaction between the F-SWCNTs and CO. Our results illustrate a new mode of sensors wherein redox active recognition units are voltageactivated to give enhanced and highly specific responses. CommunicationsRevving up for sensing: A platform for voltage-activated, chemiresistive gas detection has been delveloped based on covalently functionalized single-walled carbon nanotubes decorated with voltage-responsive chemical selectors. The sensor is highly selective towards CO in the presence of oxygen, nitrogen, and carbon dioxide and is robust to humidity and remains operational in air. KeywordsCarbon monoxide; carbon nanotubes; iron porphyrin; sensors; voltage-activated Carbon monoxide (CO) is responsible for more than half of all fatal poisoning worldwide. [1] Exposure to the colorless, tasteless, and odorless gas is difficult to discern as the initial symptoms of poisoning (headache, dizziness, and confusion) are nonspecific. In the United States, the Occupational Safety and Health Administration (OSHA) has designated permissible exposure limits of 50 ppm over eight hours and 200 ppm over five minutes. [2] The affinity of iron porphyrin towards CO is well-documented for the enzymes cytochrome P450, [3][4][5] hemoglobin, [6] and myoglobin. [7] This high affinity for CO over O 2 of hemoglobin and myoglobin is the underlying mechanism of carbon monoxide poisoning in mammals. [8,9] Although detectors for CO are available, there remains a need for massively [16,17] Theoretical and experimental reports have suggested that CO does not engage in charge transfer with pristine SWCNTs, [18][19][20] indicating the a chemical reactive interface is necessary. Conductivity...

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“…Of a 5 mM solution of freshly dissolved SNP, 50 lL was added to 50 lL of serially diluted solutions of apigenin, diosmetin and silymarin. Oxyhemoglobin reacts with NO under physiological conditions and was used as a positive control [23]. After incubation of the NO generating system with the potential scavengers for 1 h at 508C, Griess reagent was added and the concentration of nitrite was determined.…”

Section: Direct No-scavenging Effects Of Apigenin Diosmetin and Silymentioning

confidence: 99%

Plant‐derived polyphenols attenuate lipopolysaccharide‐induced nitric oxide and tumour necrosis factor production in murine microglia and macrophages

Shanmugam

Holmquist

Steele

et al. 2008

Molecular Nutrition Food Res

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Lipopolysaccharides released during bacterial infections induce the expression of pro-inflammatory cytokines and lead to complications such as neuronal damage in the CNS and septic shock in the periphery. While the initial infection is treated by antibiotics, anti-inflammatory agents would be advantageous add-on medications. In order to identify such compounds, we have compared 29 commercially available polyphenol-containing plant extracts and pure compounds for their ability to prevent LPS-induced up-regulation of NO production. Among the botanical extracts, bearberry and grape seed were the most active preparations, exhibiting IC(50) values of around 20 mug/mL. Among the pure compounds, IC(50) values for apigenin, diosmetin and silybin were 15, 19 and 12 muM, in N-11 murine microglia, and 7, 16 and 25 muM, in RAW 264.7 murine macrophages, respectively. In addition, these flavonoids were also able to down-regulate LPS-induced tumour necrosis factor production. Structure-activity relationships of the flavonoids demonstrated three distinct principles: (i) flavonoid-aglycons are more potent than the corresponding glycosides, (ii) flavonoids with a 4'-OH substitution in the B-ring are more potent than those with a 3'-OH-4'-methoxy substitution, (iii) flavonoids of the flavone type (with a C2=C3 double bond) are more potent than those of the flavanone type (with a at C2-C3 single bond).

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A theoretical study on the binding of O2, NO and CO to heme proteins

Cited by 98 publications

References 49 publications

Heme oxygenase-1 inhibits breast cancer invasion via suppressing the expression of matrix metalloproteinase-9

Heme oxygenase-1 inhibits breast cancer invasion via suppressing the expression of matrix metalloproteinase-9

Bio‐Inspired Carbon Monoxide Sensors with Voltage‐Activated Sensitivity

Plant‐derived polyphenols attenuate lipopolysaccharide‐induced nitric oxide and tumour necrosis factor production in murine microglia and macrophages

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