A Theoretical Model of pH-Based Potentiometric Biosensor Based on Immobilized Enzyme Membrane

Abstract

A theoretical model for the non steady-state response of a pH-based potentiometric biosensor immobilizing organophosphorus hydrolase (OPH) is discussed. The model is based on a system of five coupled nonlinear reaction-diffusion equations under non steady-state conditions for enzyme reactions occurring in potentiometric biosensor that describes the concentration of substrate and hydrolysis products within the membrane. New approximate analytical expressions for the concentration of the substrate (organophosphorus pesticides (OPs)) and products are derived for all values of Thiele modulus and buffer concentration using new approach of homotopy perturbation method. The analytical results are also compared with numerical ones and a good agreement is obtained. The obtained results are valid for the whole solution domain.

Investigations of Printed Flexible pH Sensing Materials Based on Graphene Platelets and Submicron RuO2Powders

“…Measuring the proton activity (pH) of environment is one of the basic chemical measurements required in various fields of human interest, like biotechnology [20], medicine [21], environmental science [22], water-support system monitoring [23], food safety [24], and many others. Apart from direct pH measurement, sensors detecting proton activity can be employed as a basis for modification, for example, with enzymes [25], nucleic acids [26], or microorganisms [27], yielding biosensors for even more applications. Given this need for sensing of pH and pH-affecting markers or reactions, improvement and development of appropriate production technologies is a relevant task for research.…”