A Thalamic Reticular Circuit for Head Direction Cell Tuning and Spatial Navigation

Vantomme, Gil; Rovó, Zita; Cardis, Romain; Béard, Elidie; Katsioudi, Georgia; Guadagno, Angelo; Perrenoud, Virginie; Fernandez, Laura M. J.; Lüthi, Anita

doi:10.1016/j.celrep.2020.107747

Cited by 31 publications

(40 citation statements)

References 85 publications

(133 reference statements)

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“…For optogenetic experiments, DBH-Cre animals were implanted with custom-made optic fibers 59 . A multimode fiber (225 μm outer diameter, Thorlabs, BFL37-2000/FT200EMT) was inserted and glued (heat-curable epoxy, Precision Fiber Products, ET-353ND-16OZ) to a multimode ceramic zirconia ferrule (Precision Fiber Products, MM-FER2007C-2300).…”

Section: Methodsmentioning

confidence: 99%

“…Thalamic brain slice recordings were performed as previously described in detail 25, 59 . Briefly, 3 – 6 weeks after viral injection, DBH-Cre mice aged 8 – 16 weeks were subjected to isoflurane anesthesia, after which they were decapitated, brains extracted and quickly immersed in ice-cold oxygenated sucrose solution (which contained in mM): NaCl 66, KCl 2.5, NaH 2 PO 4 1.25, NaHCO 3 26, D-saccharose 105, D-glucose 27, L(+)-ascorbic acid 1.7, CaCl 2 0.5 and MgCl 2 7), using a sliding vibratome (Histocom).…”

Section: Methodsmentioning

confidence: 99%

“…Slices were placed in the recording chamber of an upright microscope (Olympus BX50WI) and continuously superfused with oxygenated ACSF containing in mM: NaCl 131, KCl 2.5, NaH 2 PO 4 1.25, NaHCO 3 26, D-glucose 20, L(+)-ascorbic acid 1.7, CaCl 2 2 and MgCl 2 1.2. Cells were visualized with differential interference contrast optics and 10X and 40X immersion objectives, and their location within the thalamic ventroposterial medial nucleus or within the somatosensory reticular thalamus could be verified based on previous studies in the lab 25, 59 . Infrared images were acquired with an iXon Camera X2481 (Andor).…”

Section: Methodsmentioning

confidence: 99%

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Noradrenergic circuit control of non-REM sleep substates

Osorio-Forero

Cardis

Vantomme

et al. 2021

Preprint

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The continuity of non-rapid-eye-movement sleep (NREMS) is essential for its functions. However, many mammalian species, including humans, show NREMS fragility to maintain environmental vigilance. The neural substrates balancing NREMS continuity and fragility substates are unexplored. We show that the locus coeruleus (LC) is necessary and sufficient to generate infraslow (~50 s) continuity-fragility fluctuations in mouse NREMS. Through machine-learning-guided closed-loop optogenetic LC interrogation, we suppressed, locked, or entrained continuity-fragility fluctuations, as evident by LC-mediated regulation of sleep spindle clustering and heart rate variability. Noradrenergic modulation of thalamic but not cortical circuits was required for infraslow sleep spindle clustering and involved rapid noradrenaline increases that activated both α1- and β-adrenergic receptors to cause slowly decaying membrane depolarizations. The LC thus coordinates brain and bodily states during NREMS to engender continuity-fragility, accentuating its role in the physiology of sleep-related sensory uncoupling and as target in sleep disorders showing abnormal cortical and/or autonomic arousability.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Noradrenergic circuit control of non-REM sleep substates

Osorio-Forero

Cardis

Vantomme

et al. 2021

Preprint

Self Cite

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“…Other RSC HD neurons exhibit tuning anchored to local visual landmarks (Jacob et al, 2017). The conditions sufficient to produce this form of directional tuning are still being explored (Zhang and Jeffery, 2019) but the existence of locally anchored HD tuning could prove important for anchoring the broader HD network to the constellation of distal cues that define space within the external world (Bicanski and Burgess, 2016;Clark et al, 2010;Vantomme et al, 2020). HD cells in either PPC or RSC comprise roughly 10% of the population, although there is variability across studies.…”

Section: Coding Of Directionmentioning

confidence: 99%

Neurophysiological coding of space and time in the hippocampus, entorhinal cortex, and retrosplenial cortex

Alexander

Robinson

Dannenberg

et al. 2020

Brain and Neuroscience Advances

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Neurophysiological recordings in behaving rodents demonstrate neuronal response properties that may code space and time for episodic memory and goal-directed behaviour. Here, we review recordings from hippocampus, entorhinal cortex, and retrosplenial cortex to address the problem of how neurons encode multiple overlapping spatiotemporal trajectories and disambiguate these for accurate memory-guided behaviour. The solution could involve neurons in the entorhinal cortex and hippocampus that show mixed selectivity, coding both time and location. Some grid cells and place cells that code space also respond selectively as time cells, allowing differentiation of time intervals when a rat runs in the same location during a delay period. Cells in these regions also develop new representations that differentially code the context of prior or future behaviour allowing disambiguation of overlapping trajectories. Spiking activity is also modulated by running speed and head direction, supporting the coding of episodic memory not as a series of snapshots but as a trajectory that can also be distinguished on the basis of speed and direction. Recent data also address the mechanisms by which sensory input could distinguish different spatial locations. Changes in firing rate reflect running speed on long but not short time intervals, and few cells code movement direction, arguing against path integration for coding location. Instead, new evidence for neural coding of environmental boundaries in egocentric coordinates fits with a modelling framework in which egocentric coding of barriers combined with head direction generates distinct allocentric coding of location. The egocentric input can be used both for coding the location of spatiotemporal trajectories and for retrieving specific viewpoints of the environment. Overall, these different patterns of neural activity can be used for encoding and disambiguation of prior episodic spatiotemporal trajectories or for planning of future goal-directed spatiotemporal trajectories.

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“…that the RSC connects hippocampal formation and thalamus reciprocally, regulates the place navigation [15][16][17] and responses to nociceptive stimulations at physiological conditions [18] .…”

Section: Introductionmentioning

confidence: 99%

Single-cell RNA sequencing uncovers the excitatory/inhibitory synaptic unbalance in the retrosplenial cortex after peripheral nerve injury

Wang¹,

Chen

Lian³

et al. 2021

Preprint

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Nerve injury in the somatosensory pathway may induce maladaptive changes in the transcriptional or protein levels that contribute to the development and maintenance of neuropathic pain. The retrosplenial cortex (RSC) processes nociceptive information, and it presents structural and molecular changes after nerve injury, in contrast, the detailed transcriptional changes in the RSC were unknown. Here we first confirm the involvements of RSC in the regulation of pain sensation and observe that the same peripheral stimulation activated more splenial neurons after nerve injury; decreasing the activities of CamkIIa+ splenial cells relieves peripheral pain hypersensitivity. Using a single-cell RNA-sequencing (scRNA-seq) approach, we identify the cell-type-specific gene expressional changes after nerve injury. By analyzing the expression of ligand-gated ion channels, we observe the decreased Gabar1a but increased Gria1in the CamkIIa+ neurons; consistently, we confirm the unbalanced excitatory/inhibitory synaptic transmissions by using the electrophysiological recording approach. Our data, therefore, provide information of the cell type-dependent transcriptomic changes in the RSC after nerve injury, which would help to understand the mechanisms of mediating neuropathic pain.

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A Thalamic Reticular Circuit for Head Direction Cell Tuning and Spatial Navigation

Cited by 31 publications

References 85 publications

Noradrenergic circuit control of non-REM sleep substates

Noradrenergic circuit control of non-REM sleep substates

Neurophysiological coding of space and time in the hippocampus, entorhinal cortex, and retrosplenial cortex

Single-cell RNA sequencing uncovers the excitatory/inhibitory synaptic unbalance in the retrosplenial cortex after peripheral nerve injury

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