A tetrazolium-based colorimetric MTT assay to quantitate human monocyte mediated cytotoxicity against leukemic cells from cell lines and patients with acute myeloid leukemia

Loosdrecht, Arjan A. van de; Beelen, Robert H.J.; Ossenkoppele, GJ; Broekhoven, Marjolein G.; Langenhuijsen, M. M. A. C.

doi:10.1016/0022-1759(94)90034-5

Cited by 599 publications

(331 citation statements)

References 30 publications

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“…Taxol shows the efficacy of fungal taxol against human breast cancer cell (T47D) viability via apoptosis. The decrease in viability after taxol inclusion was assessed by MTT where it is reduced to purple formazan in the mitochondria of living cells can be measured using spectrophotometer at a wavelength between 500 and 600 nm (Van de Loosdrecht et al, 1994) Our challenge here was to study the anticancer effect of fungal taxol on human breast cancer cell line in-vitro and investigate the mechanism accordingly. As such, our data demonstrate the fungal taxol is efficacious against human breast cancer cell line with IC 50 values in the of 2.5 to 10 mM, decreased systematically with increased concentration as well as time dependent of fungal taxol compared with controls for the same exposure time which was similar to our report (Yeung et al, 1999;Kumaran et al, 2009;Pandi et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Fungal Taxol Extracted from Cladosporium oxysporum Induces Apoptosis in T47D Human Breast Cancer Cell Line

Raj

Shanmugam²,

Manikanadan³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Purpose: The present study concerns molecular mechanisms involved in induction of apoptosis by a fungal taxol extracted from the fungus Cladosporium oxysporum in T47D human breast cancer cells. Materials and Methods: Apoptosis-induced by the fungal taxol was assessed by MTT assay, nuclear staining, DNA fragmentation, flow cytometry and pro-as well as anti-apoptotic protein expression by Western blotting. Results: Our results showed inhibition of T47D cell proliferation with an IC 50 value of 2.5 μM/ml after 24 h incubation. It was suggested that the extract may exert its anti-proliferative effect on human breast cancer cell line by suppressing growth, arresting through the cell cycle, increase in DNA fragmentation as well as down-regulation of the expression of NF-ĸB, Bcl-2 and Bcl-XL and up-regulation of pro-apoptotic proteins like Bax, cyt-C and caspase-3. Conclusions: We propose that the fungal taxol contributes to growth inhibition in the human breast cancer cell through apoptosis induction via a mitochondrial mediated pathway, with possible potential as an anticancer therapeutic agent.

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Section: Discussionmentioning

confidence: 99%

“…Cytotoxicity effect was determined by MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay (Van de Loosdrecht et al, 1994;Mosmann et al, 1983). Briefly, cells were seeded into 96 well plates approximately 2×10 4 cells per well.…”

Section: Cytotoxity Evaluation By Mtt Assaymentioning

confidence: 99%

Fungal Taxol Extracted from Cladosporium oxysporum Induces Apoptosis in T47D Human Breast Cancer Cell Line

Raj

Shanmugam²,

Manikanadan³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…Cell proliferation was analyzed using the MTT assay [19] . HUVSMCs were seeded on 96-well plates at a density of 0.6-1.0×10 4 cells per well in DMEM supplemented with 10% FBS.…”

Section: Cell Proliferation Assaymentioning

confidence: 99%

Neferine inhibits angiotensin II-stimulated proliferation in vascular smooth muscle cells through heme oxygenase-1

Tong

Zhang

et al. 2010

Acta Pharmacol Sin

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Aim:To explore the effect of neferine on angiotensin II (Ang II)-induced vascular smooth muscle cell (VSMC) proliferation. Methods: Human umbilical vein smooth muscle cells (HUVSMCs) were used. Cell proliferation was determined by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry analysis. Heme oxygenase (HO)-1 protein expression was tested by Western blot analysis. Extracellular signal-regulated protein kinase 1/2 (ERK1/2) activation was determined by using immunoblotting. Results: Pre-incubation of HUVSMCs with neferine (0.1, 0.5, 1.0, and 5.0 µmol/L) significantly inhibited Ang II-induced cell proliferation in a concentration-dependent manner and neferine 5.0 µmol/L increased HO-1 expression by 259% compared with control. The antiproliferative effect of neferine was significantly attenuated by coapplication of zinc protoporphyrin IX (ZnPP IX, an HO-1 inhibitor) with neferine. Ang II-enhanced ERK1/2 phosphorylation was markedly reversed by neferine. By inhibiting HO-1 activity with ZnPP IX, the inhibitive effect of neferine on ERK1/2 phosphorylation was significantly attenuated. Cobalt-protoporphyrin (CoPP), an HO-1 inducer, significantly decreased Ang II-induced ERK1/2 phosphorylation and inhibited Ang II-induced cell proliferation. The ERK1/2 pathway inhibitor PD98059 significantly blocked Ang II-enhanced ERK1/2 phosphorylation and inhibited cell proliferation. Conclusion: These findings suggest that neferine can inhibit Ang II-induced HUVSMC proliferation by upregulating HO-1, leading to the at least partial downregulation of ERK1/2 phosphorylation.

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“…The compounds 2a-g were evaluated for their in vitro antiproliferative activity against normal human lung fibroblast MRC-5 and two human cancer cells lines: AGS gastric adenocarcinoma and HL-60 promyelocytic leukemia cells, in 72 h drugs exposure assays. The antiproliferative activity of the new compounds was measured using conventional microculture tetrazolium reduction assays [21][22][23]. The antiproliferative activity of the new quinones are expressed in terms of IC 50 (μM) and collected in Table 1.…”

Section: Scheme 1 Synthesis Of 7-anilino-1-aryl-6-bromoisoquinolineqmentioning

confidence: 99%

Antiproliferative Activity of New 6-Bromine Derivatives of 7-Anilino-1-Arylisoquinolinequinones

Ibacache

Valderrama

Arancibia

et al. 2016

J. Chil. Chem. Soc.

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A variety of 6-bromine-containing 7-anilino-1-arylisoquinolinequinones 2a-g were synthesized to evaluate their half-wave potentials and in vitro antiproliferative activity on gastric and leukemia cancer cell lines. The new compounds displayed significant IC50 values in the range: 1.31 to 11.04 µM. The structure activity relationship analysis of the new series suggest that the antiproliferative activity is dependent, in part, on the push-pull electronic effects of the nitrogen and bromine substituents inserted into the redox fragment of the 1-arylisoquinolinequinone scaffold. Linear regression analysis provided satisfactory relationships between the log IC50 and ClogP values for the AGS gastric cancer cell line.

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A tetrazolium-based colorimetric MTT assay to quantitate human monocyte mediated cytotoxicity against leukemic cells from cell lines and patients with acute myeloid leukemia

Cited by 599 publications

References 30 publications

Fungal Taxol Extracted from Cladosporium oxysporum Induces Apoptosis in T47D Human Breast Cancer Cell Line

Fungal Taxol Extracted from Cladosporium oxysporum Induces Apoptosis in T47D Human Breast Cancer Cell Line

Neferine inhibits angiotensin II-stimulated proliferation in vascular smooth muscle cells through heme oxygenase-1

Antiproliferative Activity of New 6-Bromine Derivatives of 7-Anilino-1-Arylisoquinolinequinones

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