A Tetraphenylethylene-Based Aggregation-Induced Emission Probe for Fluorescence Turn-on Detection of Lipopolysaccharide in Injectable Water with Sensitivity Down to Picomolar

Abstract

A tetraphenylethylene-based aggregation-induced emission molecule (BT-5) bearing two positively charged imidazolium pendants and an alkyl chain (C10H20) was designed as a fluorescence turn-on probe for detecting lipopolysaccharide (LPS) in injectable water sample. Upon addition of LPS, the BT-5 aqueous solution showed a concentration-dependent increase in fluorescence spectra. Moreover, BT-5 displayed a high selectivity to LPS in the presence of polysaccharide, surfactants, cations, anions, and several amino acids that may exist in injectable water samples with a low limit of detection of 120 pM, which is much lower than that of previous reports. The sensing procedure was observed by the naked eye under the 365 nm UV light irradiation. The outstanding sensing performance of BT-5 was demonstrated to originate from the combination of electrostatic and hydrophobic interactions between BT-5 and LPS.

Development of Electrospun Nanofibrous-Walled Tubes for Potential Production of Photoluminescent Endoscopes

“…17,18 Fluorescence is the capability of a material to emit light upon irradiation with an external ultraviolet light, which then returns immediately back to its origin status upon removing this light source. 19 Persistent photoluminescence can be recognized as a light emission process that has the ability to continue from a small number of seconds (phosphorescence) to a number of minutes/hours (long-persistent luminescence) upon removing the light source. 20 signs, luminous indicators, and smart clothing.…”

A Flexible Chemosensor Based on Colorimetric and Fluorescent Dual Modes for Rapid and Sensitive Detection of Hypochlorite Anion

“…A commercially available material TPE4A , namely 4′,4‴,4′′′′′,4′′′′′′′-(ethene-1,1,2,2-tetrayl)-tetrakis(([1,1′-biphenyl]-4-amine)) [ 30 , 31 ], has been purchased where the tetraphenylethylene (TPE) group shows an aggregation-induced emission (AIE) character [ 32 , 33 ]. The chemical structure of TPE4A has double checked by NMR spectra in Figure S1 .…”

Ultramicro and ultrasensitive detection of lipopolysaccharides based on triple-signal amplification via ultrafast ATRP and an ultramicroelectrode

“…9 These data clearly demonstrate that highly sensitive testing for trace LPS is a vital part of ensuring the safety of medical devices, injectable water, and related drugs due to its high toxicity to the human body. 8,10,11…”

“…9 These data clearly demonstrate that highly sensitive testing for trace LPS is a vital part of ensuring the safety of medical devices, injectable water, and related drugs due to its high toxicity to the human body. 8,10,11 The limulus amoebocyte lysate (LAL)-based assay technique is now the gold standard for LPS detection, with a sensitivity that can be as low as 0.001 EU mL −1 (∼0.1 pg mL −1 ). [12][13][14] But LAL is relatively expensive, time-consuming, and sensitive to environmental variations.…”