A TetR Family Transcriptional Factor Directly Regulates the Expression of a 3-Methyladenine DNA Glycosylase and Physically Interacts with the Enzyme to Stimulate Its Base Excision Activity in Mycobacterium bovis BCG

Liu, Lei; He, Zheng‐Guo

doi:10.1074/jbc.m113.528919

Cited by 9 publications

(4 citation statements)

References 38 publications

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“…The bioinformatics prediction together with the transcriptome analysis suggests that this protein has a regulatory role. Several regulators involved in iron sensing and control were previously described, including Fur proteins (Garcin et al ., ; Fillat, ; Krynická et al ., ), the TetR family regulator PfsR (Liu et al, ) and the non‐coding RNA IsaR1 (Georg et al ., ). However, the pattern of regulated genes affected by Slr1658 differed substantially from the previously identified iron‐stress response in Synechocystis 6803 (Hernández‐Prieto et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Resequencing of a mutant bearing an iron starvation recovery phenotype defines Slr1658 as a new player in the regulatory network of a model cyanobacterium

et al. 2017

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Photosynthetic microorganisms encounter an erratic nutrient environment characterized by periods of iron limitation and sufficiency. Surviving in such an environment requires mechanisms for handling these transitions. Our study identified a regulatory system involved in the process of recovery from iron limitation in cyanobacteria. We set out to study the role of bacterioferritin co-migratory proteins during transitions in iron bioavailability in the cyanobacterium Synechocystis sp. PCC 6803 using knockout strains coupled with physiological and biochemical measurements. One of the mutants displayed slow recovery from iron limitation. However, we discovered that the cause of the phenotype was not the intended knockout but rather the serendipitous selection of a mutation in an unrelated locus, slr1658. Bioinformatics analysis suggested similarities to two-component systems and a possible regulatory role. Transcriptomic analysis of the recovery from iron limitation showed that the slr1658 mutation had an extensive effect on the expression of genes encoding regulatory proteins, proteins involved in the remodeling and degradation of the photosynthetic apparatus and proteins modulating electron transport. Most significantly, expression of the cyanobacterial homologue of the cyclic electron transport protein PGR5 was upregulated 1000-fold in slr1658 disruption mutants. pgr5 transcripts in the Δslr1658 mutant retained these high levels under a range of stress and recovery conditions. The results suggest that slr1658 is part of a regulatory operon that, among other aspects, affects the regulation of alternative electron flow. Disruption of its function has deleterious results under oxidative stress promoting conditions.

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Section: Discussionmentioning

confidence: 99%

Resequencing of a mutant bearing an iron starvation recovery phenotype defines Slr1658 as a new player in the regulatory network of a model cyanobacterium

et al. 2017

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“…The range of TFTR controlled functions include: carbon metabolism, nitrogen metabolism, co-factor metabolism, cell to cell signalling and cell division [ 1 ]. TFTRs that do not conform to the paradigm and act as activators [ 4 – 6 ], serve as global regulators [ 7 ], interact with peptide ligands [ 8 ] and even regulate enzyme activity post-translationally [ 9 ] are being described. These observations clearly suggest that there is still much to be learned about this ubiquitous family.…”

Section: Introductionmentioning

confidence: 99%

Global analyses of TetR family transcriptional regulators in mycobacteria indicates conservation across species and diversity in regulated functions

et al. 2015

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BackgroundMycobacteria inhabit diverse niches and display high metabolic versatility. They can colonise both humans and animals and are also able to survive in the environment. In order to succeed, response to environmental cues via transcriptional regulation is required. In this study we focused on the TetR family of transcriptional regulators (TFTRs) in mycobacteria.ResultsWe used InterPro to classify the entire complement of transcriptional regulators in 10 mycobacterial species and these analyses showed that TFTRs are the most abundant family of regulators in all species. We identified those TFTRs that are conserved across all species analysed and those that are unique to the pathogens included in the analysis. We examined genomic contexts of 663 of the conserved TFTRs and observed that the majority of TFTRs are separated by 200 bp or less from divergently oriented genes. Analyses of divergent genes indicated that the TFTRs control diverse biochemical functions not limited to efflux pumps. TFTRs typically bind to palindromic motifs and we identified 11 highly significant novel motifs in the upstream regions of divergently oriented TFTRs. The C-terminal ligand binding domain from the TFTR complement in M. tuberculosis showed great diversity in amino acid sequence but with an overall architecture common to other TFTRs.ConclusionThis study suggests that mycobacteria depend on TFTRs for the transcriptional control of a number of metabolic functions yet the physiological role of the majority of these regulators remain unknown.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1696-9) contains supplementary material, which is available to authorized users.

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“…Until now, no phenotype, disease, or trait is known to be associated directly with LOC_Os06g44050 (the candidate gene of Sdt97 ), and no phenotype, disease, or trait is associated with these gene variants in plants ( www.gramene.org ). Methyladenine glycosylase has been reported only in Mycobacterium bovis BCG ( Liu et al 2014 ). Therefore, further research into Sdt97 will open new fields of research into rice semidwarfism, with emphasis on the regulation of Sdt97 expression at the transcriptional level, and the relationship between point mutations in the 5′-UTR and Sdt97 promoter, the relationship between methyladenine glycosylase and the semidwarf phenotype of the mutant, and the physiological and biochemical pathways of Sdt97 at the molecular level.…”

Section: Discussionmentioning

confidence: 99%

Sdt97: A Point Mutation in the 5′ Untranslated Region Confers Semidwarfism in Rice

Tong¹,

Han²,

Han³

et al. 2016

G3 Genes|Genomes|Genetics

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Semidwarfism is an important agronomic trait in rice breeding programs. The semidwarf mutant gene Sdt97 was previously described. However, the molecular mechanism underlying the mutant is yet to be elucidated. In this study, we identified the mutant gene by a map-based cloning method. Using a residual heterozygous line (RHL) population, Sdt97 was mapped to the long arm of chromosome 6 in the interval of nearly 60 kb between STS marker N6 and SNP marker N16 within the PAC clone P0453H04. Sequencing of the candidate genes in the target region revealed that a base transversion from G to C occurred in the 5′ untranslated region of Sdt97. qRT-PCR results confirmed that the transversion induced an obvious change in the expression pattern of Sdt97 at different growth and developmental stages. Plants transgenic for Sdt97 resulted in the restoration of semidwarfism of the mutant phenotype, or displayed a greater dwarf phenotype than the mutant. Our results indicate that a point mutation in the 5′ untranslated region of Sdt97 confers semidwarfism in rice. Functional analysis of Sdt97 will open a new field of study for rice semidwarfism, and also expand our knowledge of the molecular mechanism of semidwarfism in rice.

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A TetR Family Transcriptional Factor Directly Regulates the Expression of a 3-Methyladenine DNA Glycosylase and Physically Interacts with the Enzyme to Stimulate Its Base Excision Activity in Mycobacterium bovis BCG

Cited by 9 publications

References 38 publications

Resequencing of a mutant bearing an iron starvation recovery phenotype defines Slr1658 as a new player in the regulatory network of a model cyanobacterium

Resequencing of a mutant bearing an iron starvation recovery phenotype defines Slr1658 as a new player in the regulatory network of a model cyanobacterium

Global analyses of TetR family transcriptional regulators in mycobacteria indicates conservation across species and diversity in regulated functions

Sdt97: A Point Mutation in the 5′ Untranslated Region Confers Semidwarfism in Rice

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