A TetR-family protein activates transcription from a new promoter motif associated with essential genes for autotrophic growth in acetogens

Abstract

Acetogens can fix carbon (CO or CO2) into acetyl-CoA via the Wood-Ljungdahl pathway (WLP) that also makes them attractive cell factories for the production of fuels and chemicals from waste feedstocks. Although most biochemical details of the WLP are well understood and systems-level characterisation of acetogen metabolism has recently improved, key transcriptional features such as promoter motifs and transcriptional regulators are still unknown in acetogens. Here, we use differential RNA-sequencing to identify a previously undescribed promoter motif associated with essential genes for autotrophic growth of the model-acetogen Clostridium autoethanogenum. RNA polymerase was shown to bind to the new promoter motif using a DNA-binding protein assay and proteomics enabled the discovery of four candidates to potentially function directly in control of transcription of the WLP and other key genes of C1 fixation metabolism. Next, in vivo experiments showed that a TetR-family transcriptional regulator (CAETHG_0459) and the housekeeping sigma factor (σA) activate expression of a reporter protein (GFP) in-frame with the new promoter motif from a fusion vector in E. coli. Lastly, a protein-protein interaction assay with the RNA polymerase (RNAP) shows that CAETHG_0459 directly binds to the RNAP. Together, the data presented here advance the fundamental understanding of transcriptional regulation of C1 fixation in acetogens and provide a strategy for improving the performance of gas-fermenting bacteria by genetic engineering.

Characterisation of the butyrate production pathway in probiotic MIYAIRI588 by a combined whole genome-proteome approach

“…One recent study investigating the transcriptome of Clostridioides difficile strain 630 identified the RBS to be aGGAGg for the majority of genes for which transcripts could be detected (64) suggesting high conservation of this sequence per se in this species. A similar finding was made for Clostridium autoethanogenum (65). Prior to the development of omic tools, studies focusing on individual genes have also reported AGGAGG as the likely RBS for other species of Clostridium including the type E toxin gene of C. botulinum (66), colA encoding collagenase of C. perfringens (67), celA gene encoding endoglucanase A of C. themocellum (68) to name but a few.…”