A tethered bilayer lipid membrane that mimics microbial membranes

Andersson, Jakob; Fuller, Melanie A.; Wood, Kathleen; Holt, Stephen A.; Köper, Ingo

doi:10.1039/c8cp01346b

Cited by 40 publications

(45 citation statements)

References 44 publications

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“…The frequency range for our EIS measurements precluded the influence of cytoplasm properties, since it has been reported that the cytoplasm conductivity becomes prevalent at frequencies approaching 10 MHz (10). The difference in capacitance that we measured could indicate, for example, differences in the composition of the phospholipids or the composition of membrane proteins that could have altered the dielectric properties of the cell membrane (11)(12)(13). The measurement of capacitance is sensitive to changes in the dielectric properties, and hence our measurements of capacitance may provide a measurement of the difference in composition of the membranes of cancerous cells compared to normal cells.…”

Section: Discussionmentioning

confidence: 91%

Improved micro-impedance spectroscopy to determine cell barrier properties

Sohag

Nicoud

Amine

et al. 2020

The EuroBiotech Journal

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The goal of this study was to determine whether the Tethapod system, which was designed to determine the impedance properties of lipid bilayers, could be used for cell culture in order to utilise micro-impedance spectroscopy to examine further biological applications. To that purpose we have used normal epithelial cells from kidney (RPTEC) and a kidney cancer cell model (786-O). We demonstrate that the Tethapod system is compatible with the culture of 10,000 cells seeded to grow on a small area gold measurement electrode for several days without affecting the cell viability. Furthermore, the range of frequencies for EIS measurements were tuned to examine easily the characteristics of the cell monolayer. We demonstrate significant differences in the paracellular resistance pathway between normal and cancer kidney epithelial cells. Thus, we conclude that this device has advantages for the study of cultured cells that include (i) the configuration of measurement and reference electrodes across a microfluidic channel, and (ii) the small surface area of 6 parallel measurement electrodes (2.1 mm2) integrated in a microfluidic system. These characteristics might improve micro-impedance spectroscopy measurement techniques to provide a simple tool for further studies in the field of the patho-physiology of biological barriers.

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Section: Discussionmentioning

confidence: 91%

Improved micro-impedance spectroscopy to determine cell barrier properties

Sohag

Nicoud

Amine

et al. 2020

The EuroBiotech Journal

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“…This work was the first synthetic strategy to obtain compositionally asymmetric multilamellar structures. Köper and co‐workers reported the fabrication of a tethered bilayer lipid membrane which can mimics the biomembranes . Through their efforts, different membranes properties, such as highly stable and highly resistive bilayers, can be obtained by varying lipid composition and tethering density.…”

Section: Synthesizing Artificial Cells By Microfluidicsmentioning

confidence: 99%

Microfluidics for Biosynthesizing: from Droplets and Vesicles to Artificial Cells

Xie

Xiong

et al. 2019

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112

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Fabrication of artificial biomimetic materials has attracted abundant attention. As one of the subcategories of biomimetic materials, artificial cells are highly significant for multiple disciplines and their synthesis has been intensively pursued. In order to manufacture robust “alive” artificial cells with high throughput, easy operation, and precise control, flexible microfluidic techniques are widely utilized. Herein, recent advances in microfluidic‐based methods for the synthesis of droplets, vesicles, and artificial cells are summarized. First, the advances of droplet fabrication and manipulation on the T‐junction, flow‐focusing, and coflowing microfluidic devices are discussed. Then, the formation of unicompartmental and multicompartmental vesicles based on microfluidics are summarized. Furthermore, the engineering of droplet‐based and vesicle‐based artificial cells by microfluidics is also reviewed. Moreover, the artificial cells applied for imitating cell behavior and acting as bioreactors for synthetic biology are highlighted. Finally, the current challenges and future trends in microfluidic‐based artificial cells are discussed. This review should be helpful for researchers in the fields of microfluidics, biomaterial fabrication, and synthetic biology.

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“…Neutron scattering showed that using either the self-diluting lipids DPhySDL or DPhyHDL or inner leaflets diluted with mercaptoethanol increases the sub-membrane hydration from 5% to around 25%. Using partially deuterated phospholipids it was demonstrated that when the proximal leaflet is diluted, phospholipids also incorporate into the inner leaflet of the membrane (Andersson et al, 2018).…”

Section: Sparsely Tethered Membranesmentioning

confidence: 99%

“…When a metallic supporting surface is used, surface plasmon resonance (SPR) and surface-plasmon enhanced fluorescence spectroscopy (SPFS) can also be used to study membrane-related processes within 150-200 nm of the substrate surface (Wiltschi et al, 2006). This makes SPFS and SPR highly suitable for the study of tethered and supported lipid bilayer membranes which typically have a thickness of around 8-20 nm Junghans and Koper, 2010;Andersson et al, 2018). When using gold as a substrate, an excitation wavelength of 520 nm or above must be used for SPFS, which has to be considered when selecting fluorophores for the experiment.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, tethered membranes can serve as stable and highly flexible platforms for biosensing applications (Cornell et al, 1997;Jackman et al, 2012). Model membranes have also been developed that mimic the outer membrane of gram-negative bacteria, which can cause some of the most dangerous multi-drug resistant infections (Clifton et al, 2015;Andersson et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Tethered Membrane Architectures—Design and Applications

2018

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Membrane proteins perform a large number of essential biological tasks, but the understanding of these proteins has progressed much more slowly than that of globular proteins. The study of membrane proteins is hindered by the inherent complexity of the cellular membrane. Membrane proteins cannot be studied outside their native environment because their natural structure and function is compromised when the protein does not reside in the cell membrane. Model membranes have been developed to provide a controlled, membrane-like environment in which these proteins can be studied in their native form and function without interference from other membrane components. Traditionally used model membranes such as bimolecular or black lipid membranes and floating lipid membranes suffer from several disadvantages including complex assembly protocols and limited stability. Furthermore, these membranes can only be studied with a narrow range of methodologies, severely restricting their use. To increase membrane stability, simplify the assembly process and increase the number of analytical tools that can be used to study the membranes, several strategies of covalently tethering the bilayer to its solid support have been developed. This review provides an overview of the methods used to assemble various membrane architectures, the properties of the resulting membranes and the tools used to study them.

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A tethered bilayer lipid membrane that mimics microbial membranes

Cited by 40 publications

References 44 publications

Improved micro-impedance spectroscopy to determine cell barrier properties

Improved micro-impedance spectroscopy to determine cell barrier properties

Microfluidics for Biosynthesizing: from Droplets and Vesicles to Artificial Cells

Tethered Membrane Architectures—Design and Applications

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