A test strip platform based on a whole-cell microbial biosensor for simultaneous on-site detection of total inorganic mercury pollutants in cosmetics without the need for predigestion

Guo, Mingzhang; Wang, Jili; Du, Ruoxi; Liu, Yanger; Chi, Jiani; He, Xiaoyun; Huang, Kunlun; Luo, Yunbo; Xu, Wentao

doi:10.1016/j.bios.2019.111899

Cited by 55 publications

(22 citation statements)

References 28 publications

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“…Metal binding domains derived from MerR-like regulators were demonstrated to retain target metal ion binding capacities, and have been efficiently displayed on the microbial cell surface for heavy metal remediation [ 11 , 16 , 36 , 41 , 42 ]. Bioadsorption and biosensing of toxic mercury were previously achieved using surface display of mercury binding proteins [ 11 , 12 , 41 ] and whole-cell biosensing techniques [ 7 , 9 ], respectively. Integration of mercury bioadsorption and biosensing in a single engineered bacterial cell was realized using an artificial double-promoter regulated mer operon in the study.…”

Section: Resultsmentioning

confidence: 99%

“…The GFP fluorescence emission showed a linear increase from 100 to 1700 nM Hg(II) in an E. coli biosensor [8]. A linear positive correlation was observed between 50 nM to 10 μM Hg(II) in an engineered E. coli with constitutively expressed MerR as a sensor protein and inducible mCherry as the reporter [9]. However, no whole-cell biosensors have been developed to be used in the upper ranges of Hg(II) concentrations where linearity was demonstrated currently.…”

Section: Mercury Sensitivity Detection With Single-and Double-fluorescent Signal Biosensorsmentioning

confidence: 99%

“…Several whole-cell biosensors to detect bioavailable Hg(II) were successfully developed using the Hg(II) response elements originating from the natural mer operon. These single-signal output biosensor constructs responded to bioavailable Hg(II) by producing light, β-galactosidase, fluorescent protein, or pigment [7][8][9][10]. Due to its high affinity and selectivity toward Hg(II), MerR has been genetically engineered onto the surface of bacteria to develop microbial biosorbents specific for Hg(II) removal [11,12].…”

Section: Introductionmentioning

confidence: 99%

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Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury

Zhang

Guo

et al. 2021

PLoS ONE

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Mercury exists naturally and mainly as a man-made pollutant in the environment, where it exerts adverse effects on local ecosystems and living organisms. It is important to develop an appropriate synthetic biological device that recognizes, detects and removes the bioavailable fraction of environmental mercury. Both single-signal and double-signal output mercury biosensors were assembled using a natural mer operon as a template. Selectivity and sensitivity of whole-cell biosensors based on artificial mer operons were determined. Three whole-cell biosensors were highly stable at very high concentrations of mercuric chloride, and could detect bioavailable Hg(II) in the concentration range of 6.25–200 μM HgCl2. A novel Hg(II) bioadsorption coupled with biosensing artificial mer operon was assembled. This would allow Hg(II)-induced Hg(II) binding protein cell surface display and green fluorescence emission to be achieved simultaneously while retaining the linear relationship between fluorescent signal and Hg(II) exposure concentration. The present study provides an innovative way to simultaneously detect, quantify, and remove bioavailable heavy metal ions using an artificially reconstructed heavy metal resistance operon.

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Section: Resultsmentioning

confidence: 99%

Section: Mercury Sensitivity Detection With Single-and Double-fluorescent Signal Biosensorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury

Zhang

Guo

et al. 2021

PLoS ONE

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“…Fluorescence intensity showed an excellent linear relationship in the range of 50 nM to 10 μM mercury concentration (Figure 9). 139 In combination with the inhibition of the hydrogen peroxide catalysis reaction, the amperometric technique was proposed for the indirect detection of divalent mercury ions. In this process, a glassy carbon electrode was applied as the biosensor's functional substrate, the surface of which was modified by the catalase using cross-linking with glutaraldehyde and bovine serum albumin.…”

Section: Mercurymentioning

confidence: 99%

Recent Advances in Nanotechnology-Based Biosensors Development for Detection of Arsenic, Lead, Mercury, and Cadmium

Maghsoudi¹,

Hassani²,

Mirnia³

et al. 2021

IJN

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Heavy metals cause considerable environmental pollution due to their extent and non-degradability in the environment. Analysis and trace levels of arsenic, lead, mercury, and cadmium as the most toxic heavy metals show that they can cause various hazards in humans’ health. To achieve rapid, high-sensitivity methods for analyzing ultra-trace amounts of heavy metals in different environmental and biological samples, novel biosensors have been designed with the participation of strategies applied in nanotechnology. This review attempted to investigate the novel, sensitive, efficient, cost-benefit, point of care, and user-friendly biosensors designed to detect these heavy metals based on functional mechanisms. The study’s search strategies included examining the primary databases from 2015 onwards and various keywords focusing on heavy metal biosensors’ performance and toxicity mechanisms. The use of aptamers and whole cells as two important bio-functional nanomaterials is remarkable in heavy metal diagnostic biosensors’ bioreceptor design. The application of hybridized nanomaterials containing a specific physicochemical function in the presence of a suitable transducer can improve the sensing performance to achieve an integrated detection system. Our study showed that in addition to both labeled and label-free detection strategies, a wide range of nanoparticles and nanocomposites were used to modify the biosensor surface platform in the detection of heavy metals. The detection limit and linear dynamic range as an essential characteristic of superior biosensors for the primary toxic metals are studied. Furthermore, the perspectives and challenges facing the design of heavy metal biosensors are outlined. The development of novel biosensors and the application of nanotechnology, especially in real samples, face challenges such as the capability to simultaneously detect multiple heavy metals, the interference process in complex matrices, the efficiency and stability of nanomaterials implemented in various laboratory conditions.

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“…A glucose electrochemical biosensor using wax-defined paper with diluted carbon ink was presented in [21]. A biosensor for the detection of mercury pollution in cosmetics, based on visual effects on a disk-shaped piece of filter paper, was developed in [22]. A paper-based device created by graphite pencil leads and modified with nafion was proposed in [23] and applied for the detection of pyocyanin, a biomarker for Pseudomonas aeruginosa.…”

Section: Introductionmentioning

confidence: 99%

A Functionalized Paper Strip‐Based Platform for Rapid Detection of Anticancer Drug Concentrations

et al. 2021

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This article presents a compact, low-cost, robust, and flexible test platform for determining the concentration of drugs in fluids. This device is based on a PVC foil card containing copper conductive foils in which disposable paper test strips are inserted. The paper test strips are overlaid with deposition of multiwalled carbon nanotubes (MWCNTs) acting as a sensing layer. When a paper test strip is inserted into the test card, the conductive path between two copper lines is established. A drop of test fluid on the sensing MWCNT layer changes the conductivity in a concentration-dependent manner, enabling calculation of the drug concentration after measurement of the electrical resistance at the copper terminals. An equivalent electrical circuit was also proposed to model the response of the fabricated sensor. It was shown that model parameters are dependent on the concentration of the cytostatic drug methotrexate. Additionally, the fabricated sensor demonstrated the ability to differentiate the same concentration of methotrexate and cyclophosphamide. The complete readout electronics and polynomial transfer function for calculating drug concentrations were also developed and are presented.

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A test strip platform based on a whole-cell microbial biosensor for simultaneous on-site detection of total inorganic mercury pollutants in cosmetics without the need for predigestion

Cited by 55 publications

References 28 publications

Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury

Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury

Recent Advances in Nanotechnology-Based Biosensors Development for Detection of Arsenic, Lead, Mercury, and Cadmium

A Functionalized Paper Strip‐Based Platform for Rapid Detection of Anticancer Drug Concentrations

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