A tentative mechanism for the anaerobic transport of glucose, fructose and mannose in yeast

Scharff, Thomas G.; Kremer, Eugene H.

doi:10.1016/0003-9861(62)90064-4

Cited by 20 publications

(5 citation statements)

References 17 publications

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“…The Km of intact yeast cells, i.e., of nonpoisoned cells, for glucose, fructose, and galactose determined by numerous investigations with different methods (6,19,20,(22)(23)(24) are of the same magnitude as ours (5 and this paper), with the exception of the results of Scharff and Kremer (19), who reported a five times higher value for the Km of yeast for glucose and fructose. From our results and those of Sols (20) and Blackley and Boyer (6), it seems that yeasts have a higher affinity for glucose than for fructose and galactose.…”

Section: Discussionsupporting

confidence: 67%

“…Although our results concerning the Michaelis constants are in agreement with most of the literature data, the same is not true for the Vmax values. Our Vmax values are, in general, between 10 and 50 times higher than other published values (19,22,23). We think that the low Vmax values obtained by numerous investigators are due to various treatments of the cells before kinetic determinations.…”

Section: Discussioncontrasting

confidence: 58%

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Microcalorimetric Determination of the Affinity of Saccharomyces cerevisiae for Some Carbohydrate Growth Substrates

Murgier

Bélaı̈ch

1971

J Bacteriol

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Section: Discussionsupporting

confidence: 67%

Section: Discussioncontrasting

confidence: 58%

Microcalorimetric Determination of the Affinity of Saccharomyces cerevisiae for Some Carbohydrate Growth Substrates

Murgier

Bélaı̈ch

1971

J Bacteriol

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“…Neither the glucose nor the galactose system will accept sorbose, and the glucose system will not accept galactose. The glucose system is also used by fructose and mannose which are glucose competitors (2).…”

Section: Discussionmentioning

confidence: 99%

Sugar Transport and Metal Binding in Yeast

Steveninck

Rothstein

1965

The Journal of General Physiology

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The uptake of sugars by yeast can be separated into two classes. The first involves the uptake of sorbose or galactose by starved cells, and the uptake of glucose by iodoacetate-poisoned cells. These uptakes do not involve any changes in Ni + + -or Co++-binding by the cell surface, are not inhibited by Ni++, are inhibited by UO ++ in relatively high concentrations, are characterized by high Michaelis constants and low maximal rates and by a final equilibrium distribution of the sugars. The second involves the uptake of glucose in unpoisoned cells and galactose in induced cells. These uptakes are characterized by a reduction of Ni + + -and Co++-binding, by a partial inhibition by Ni++, by an inhibition with UO2 ++ in relatively low concentrations, and by a low Km and a high 'm. In the case of galactose in induced cells, previous studies demonstrate that the sugar is accumulated against a concentration gradient. It is suggested that the first class of uptakes involves a "facilitated diffusion" via a relatively non-specific carrier system, but the second represents an "uphill" transport involving the highly specific carriers, and phosphoryl groups (cation-binding sites) of the outer surface of the cell membrane.From studies of kinetic properties and specificity patterns, it has been concluded that sugar uptake in yeast as in other cells, involves a relatively specific combination of the sugar with a limited number of membrane sites or "carriers" (1, 2). The carrier pattern, however, can apply to two kinds of sugar transport. In one, the driving force for sugar movement is the concentration gradient of sugar across the membrane in which the net movement of sugar ceases when equilibrium distribution is attained. A prototype system is the equilibration of sugars across the red cell membrane, often called "facilitated diffusion" (3). In the other, the metabolism of the cell provides the energy to drive the sugar in the "uphill" direction, resulting in non-equilibrium distributions and large accumulation ratios. The term "active transport" is usually applied in this case. A prototype is the "permease" system for galactosides in E. coli (4).In yeast exposed to fermentable sugars such as glucose and fructose, despite high rates of uptake, little or no sugar can normally be detected within the 235

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“…The stoichiometric constants of yeast growth may be evaluated from yield figures, combined with suitable mass balances: yield on glucose = 0.15 g cells/g glucose = l/al (fermentation only) a1 = 6.67 yield on ethanol = 0.45 g cells/g ethanol = l/aa (respiration only) a3 = 2 22. …”

mentioning

confidence: 99%

A mechanistic model of the aerobic growth of Saccharomyces cerevisiae

Bijkerk

Hall

1977

Biotech & Bioengineering

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A two-stage deterministic model of the growth of Saccharomyces cerevisiae is presented. The cell cycle of this organism was used to suggest the basic model structure. The model represents the preparatory processes of substrate uptake and conversion separately from replication and division. The regulation of the fraction of the culture devoted to each of these broad areas of metabolism, and the overall growth rate, is related to the nature and availability of the energy substrate. The simulation of respiration and glycolysis is achieved by including two alternative energy producing pathways. The regulation of these pathways is described in terms of the postulated primary regulation of the proportion of the culture required for substrate uptake and conversion, and the overall kinetic constants for each pathway. This regulation is dictated primarily by the growth rate rather than the nature or concentration of the energy substrate. The model successfully describes both batch and continuous growth of S. cerevisiae under conditons of glucose limitation and oxygen excess. A preliminary assessment indicates that adjustment of the relevant parameters will allow the model to describe the growth of S. cerevisiae on other sugars and under oxygen limitation. Similarly the model could be expected to describe the growth characteristics of other yeast species.

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A tentative mechanism for the anaerobic transport of glucose, fructose and mannose in yeast

Cited by 20 publications

References 17 publications

Microcalorimetric Determination of the Affinity of Saccharomyces cerevisiae for Some Carbohydrate Growth Substrates

Microcalorimetric Determination of the Affinity of Saccharomyces cerevisiae for Some Carbohydrate Growth Substrates

Sugar Transport and Metal Binding in Yeast

A mechanistic model of the aerobic growth of Saccharomyces cerevisiae

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