A ten-year experience with bacterial aortitis

Öz, Mehmet C.; Brener, Bruce J.; Buda, J; Todd, George J.; Brenner, Richard W.; Goldenkranz, Robert J.; McNicholas, Kathleen W.; Lemole, Gerald M.; Lozner, Jerome S.

doi:10.1016/0741-5214(89)90419-9

Cited by 125 publications

(76 citation statements)

References 20 publications

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“…aureus infects elastin-rich tissues such as lung and heart valves, causing pneumonia and native-valve endocarditis, respectively (15,16). The membrane-associated elastin-binding protein (EbpS) was shown to promote binding of soluble elastin peptides and tropoelastin to S. aureus cells (17,18).…”

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confidence: 99%

The N-terminal A Domain of Fibronectin-binding Proteins A and B Promotes Adhesion of Staphylococcus aureus to Elastin

Roche

Downer

Keane

et al. 2004

Journal of Biological Chemistry

124

119

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The ability of Staphylococcus aureus to adhere to components of the extracellular matrix is an important mechanism for colonization of host tissues during infection. We have previously shown that S. aureus binds elastin, a major component of the extracellular matrix. The integral membrane protein, elastin-binding protein (EbpS), binds soluble elastin peptides and tropoelastin via its surface-exposed N-terminal domain. In this study, we demonstrate that some strains of S. aureus adhere strongly to immobilized human elastin and that this interaction is independent of EbpS but instead is mediated by the fibronectin-binding proteins, FnBPA and FnBPB. Our results show that EbpS mutant cells adhere to elastin-coated plates, whereas the cells negative for FnBPA and FnBPB do not adhere to the plates. Furthermore, only wild-type cells from the exponential phase of growth adhered when FnBPs were expressed maximally. We show that adherence to elastin promoted by FnBPA was not affected by soluble fibronectin, suggesting that the elastin binding domain is distinct from the fibronectin binding regions. Recombinant FnBPA 37-544 (rFnBPA 37-544 ) protein corresponding to the A region of FnBPA and anti-FnBPA 37-544 antibodies inhibited FnBPA-mediated bacterial adherence to immobilized elastin. Finally, recombinant A domain proteins, rFnBPA 37-544 and rFnBPB 37-540 , bound immobilized elastin dose-dependently and saturably. This interaction was inhibited by soluble elastin peptides, suggesting a specific receptor-ligand interaction.

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mentioning

confidence: 99%

The N-terminal A Domain of Fibronectin-binding Proteins A and B Promotes Adhesion of Staphylococcus aureus to Elastin

Roche

Downer

Keane

et al. 2004

Journal of Biological Chemistry

124

119

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“…7,9,10,16,17 Life-long suppressive antibiotics have also been recommended. 18,19 Two traditional methods of revascularization have been described in the literature. These are in situ grafting and extra-anatomical bypass grafting using synthetic materials.…”

Section: Discussionmentioning

confidence: 99%

Salmonella Aortitis: A Case Report

Al‐Tawfiq

Khougeer

2002

Ann Saudi Med

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Endothelial infection is a recognized complication of Salmonella bacteremia, especially in elderly patients with diabetes mellitus. Mycotic aneurysms usually involve the abdominal aorta and may involve the iliac arteries.1,2 The clinical presentation of mycotic aneurysm secondary to Salmonella is classic (fever, back pain, and/or abdominal pain) but nonspecific. Such aneurysms are associated with high morbidity and mortality. We present a case of aortic mycotic aneurysm due to Salmonella and outline the therapeutic options. Case ReportA 71-year-old man known to have coronary artery disease, diabetes mellitus and hypertension, was admitted for management of Salmonella bacteremia. A week prior to admission, the patient had developed fever, chills and rigors, and had been treated symptomatically. Blood cultures that were taken grew Salmonella non-typhi group D four days later, and oral azithromycin was prescribed. The patient continued to have fever and chills, and was hence admitted for further evaluation.On examination, his temperature was 38°C, and there was no palpable lymphadenopathy. Chest and heart examination was unremarkable and no murmur was detected. The abdomen was soft and lax with no palpable masses. Laboratory investigations revealed a WBC of 15x10 9 /L with 60% polymorphonuclear cells, hemoglobin of 7.44 mmol/L (12 g/dL), and platelets of 656x10 9 /L. ESR was 110 and C-reactive-protein (CRP) was 22.6. Initial blood cultures one week prior to admission and those on admission were positive for S. nontyphi group D. Ceftriaxone was started intravenously. The patient complained of back pain and continued to have a low-grade fever. MRI of the spine revealed spondylolithiasis at L5-S1 and a hemangioma at LI with no herniation of the disc. A bone scan was negative, however, gallium-67 scan revealed a focal area of accumulation of the radio-tracer at the region of L4-5 area. CT scan of the abdomen revealed an aneurysmal dilatation of the abdominal aorta, extending from below the origin of the renal arteries down to the iliac bifurcation. The aneurysm demonstrated an extraluminal leakage beyond the calcified wall (Figure 1). The patient underwent successful resection and grafting of the abdominal aortic aneurysm using a straight 14 mm Gore-Tex graft. Intraoperatively, the small bowel was adherent to the site of the aortic aneurysm and was technically difficult to separate from the aneurysm, hence the native aortic tissue was left in place. Intraoperative Gram's stain of the aneurysmal wall showed many WBCs but no organisms, and the cultures subsequently were negative. A repeat CT scan one week postoperatively showed no leak from the aorta. The CRP decreased to 12.6 and ESR to 64. The patient received IV ceftriaxone for a total of 38 days, and a two-month course of oral Ciprofloxacin. On follow-up, the CRP was <0.3 and ESR was 9. At one-year follow-up, the patient was asymptomatic with negative blood cultures and good anatomic results on CT scan. DiscussionIn 1885, Osier described mycotic aneurysm in associati...

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“…S. aureus is one of the causative agents of diseases such as infective endocarditis, osteomyelitis, aortitis, pneumonia, and scalded skin syndrome (13)(14)(15). Furthermore, several strains of S. aureus tend to extravasate into the circulation to cause bacteremia and subsequent formation of metastatic abscesses.…”

Section: The Extracellular Matrix (Ecm)mentioning

confidence: 99%

Molecular Cloning and Expression of the Gene for Elastin-binding Protein (ebpS) in Staphylococcus aureus

Park

Rosenbloom

Abrams

et al. 1996

Journal of Biological Chemistry

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Interactions between staphylococci and components of the extracellular matrix mediate attachment of the bacteria to host tissues and organs and define an important mechanism leading to colonization, invasion, and formation of metastatic abscesses. We have previously demonstrated a specific binding interaction between Staphylococcus aureus and elastin, one of the major protein components of the extracellular matrix. Available evidence suggests that this association is mediated by a 25-kDa elastin-binding protein on the surface of S. aureus (EbpS). To study the molecular structure and function of EbpS, the gene encoding EbpS was cloned, sequenced, and expressed in Escherichia coli.

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A ten-year experience with bacterial aortitis

Cited by 125 publications

References 20 publications

The N-terminal A Domain of Fibronectin-binding Proteins A and B Promotes Adhesion of Staphylococcus aureus to Elastin

The N-terminal A Domain of Fibronectin-binding Proteins A and B Promotes Adhesion of Staphylococcus aureus to Elastin

Salmonella Aortitis: A Case Report

Molecular Cloning and Expression of the Gene for Elastin-binding Protein (ebpS) in Staphylococcus aureus

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