A Temporary Immersion System (Tis) Bioreactor Used for the in Vitro Propagation of Prunus and Pyrus Rootstocks

Abstract

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GreenTray®, a TIS Bioreactor for Plant Micropropagation and Abiotic or Biotic Stress Bioassays

“…The extraction of shoots with forceps from in vitro culture containers, as well as from TIS bioreactors, followed by arranging them on sterile surfaces in order to divide them with the help of surgical scalpels and their subsequent introduction into new culture containers, a process known as subculturing in the conventional in vitro culture process, is time-consuming and represents a significant portion the hand labor involved in most in vitro plant culture processes. The components of the actual GreenTray ® TIS bioreactor described herein, which is a further evolution of the ones presented in the past [31,32], make it significantly improved and versatile compared to other commercial TIS bioreactors, such as RITA ® , Matis ® , Plantform TM , SETIS TM [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17], or the TIS developed by several in vitro plant culture laboratories oriented to solve particular propagation objectives for diverse plant species [18][19][20][21][22][23][24][25][26][27][28].…”

“…The patented GreenTray ® [29,30] design is characterized by the ability to access the interior of the plant cultivation container. Its design derives from a previously handmade assemblage [31], and another adaptation of a plastic container [32]. When opening the cultivation container of the actual GreenTray ® , the lid and the cultivation tray are always joined, and therefore, the sprouts or plants in cultivation are oriented with the movement of the lid, with just one hand.…”

GreenTray^®, a TIS Bioreactor for Plant Micropropagation and Abiotic or Biotic Stress Bioassays

“…Extraction of shoots with forceps form the in vitro culture containers, as well as from TIS bioreactors, arranging them on sterile surfaces in order to divide them with the help of surgical scalpels, followed by their introduction into new culture containers, process known as performing a subculture in the conventional in vitro culture process, is time consuming and represents great part of the hand labor expense dedicated to the perform most of the plant in vitro culture processes. The components of the actual GreenTray ® TIS bioreactor described herein, which is a further evolution of the ones presented in the past [32,33], make it significantly improved and versatile compared to other commercial TIS bioreactors, such as RITA ® , Matis ® , Plantform TM , SETIS TM [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20], or the TIS developed by several plant invitro culture laboratories oriented to solve particular propagation objectives for diverse plant species [21][22][23][24][25][26][27][28][29][30][31].…”

“…The patented GreenTray ® [2,3] design is characterized in the way of accessing the interior of the plant cultivation container. Its design derives from a previously handmade assemblage [32], and another adaptation to a plastic container [33]. When opening the cultivation container of the actual GreenTray ® , the lid and the cultivation tray are always joined, and therefore the sprouts or plants in cultivation are oriented with the movement of the lid, with just one hand.…”