A temperature-shift strategy for efficient arachidonic acid fermentation by Mortierella alpina in batch culture

Peng, Chao; Huang, He; Ji, Xiao‐Jun; Liu, Xin; You, Jiangying; Lu, Jinmiao; Cong, Leilei; Xu, Xiaokang; Ouyang, Pingkai

doi:10.1016/j.bej.2010.09.014

Cited by 37 publications

(24 citation statements)

References 20 publications

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“…This was consistent with our early study with this strain using N-acetyl-glucosamine as the substrate [29]. However, opposite trends were also found in the case of polyunsaturated fatty acids production by the filamentous fungus Mortierella alpine [30]. …”

Section: Resultssupporting

confidence: 92%

Efficient conversion of biomass into lipids by using the simultaneous saccharification and enhanced lipid production process

Gong

Shen

Wang

et al. 2013

Biotechnol Biofuels

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BackgroundMicrobial lipid production by using lignocellulosic biomass as the feedstock holds a great promise for biodiesel production and biorefinery. This usually involves hydrolysis of biomass into sugar-rich hydrolysates, which are then used by oleaginous microorganisms as the carbon and energy sources to produce lipids. However, the costs of microbial lipids remain prohibitively high for commercialization. More efficient and integrated processes are pivotal for better techno-economics of microbial lipid technology.ResultsHere we describe the simultaneous saccharification and enhanced lipid production (SSELP) process that is highly advantageous in terms of converting cellulosic materials into lipids, as it integrates cellulose biomass hydrolysis and lipid biosynthesis. Specifically, Cryptococcus curvatus cells prepared in a nutrient-rich medium were inoculated at high dosage for lipid production in biomass suspension in the presence of hydrolytic enzymes without auxiliary nutrients. When cellulose was loaded at 32.3 g/L, cellulose conversion, cell mass, lipid content and lipid coefficient reached 98.5%, 12.4 g/L, 59.9% and 204 mg/g, respectively. Lipid yields of the SSELP process were higher than those obtained by using the conventional process where cellulose was hydrolyzed separately. When ionic liquid pretreated corn stover was used, both cellulose and hemicellulose were consumed simultaneously. No xylose was accumulated over time, indicating that glucose effect was circumvented. The lipid yield reached 112 mg/g regenerated corn stover. This process could be performed without sterilization because of the absence of auxiliary nutrients for bacterial contamination.ConclusionsThe SSELP process facilitates direct conversion of both cellulose and hemicellulose of lignocellulosic materials into microbial lipids. It greatly reduces time and capital costs while improves lipid coefficient. Optimization of the SSELP process at different levels should further improve the efficiency of microbial lipid technology, which in turn, promote the biotechnological production of fatty acid-derived products from lignocellulosic biomass.

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Section: Resultssupporting

confidence: 92%

Efficient conversion of biomass into lipids by using the simultaneous saccharification and enhanced lipid production process

Gong

Shen

Wang

et al. 2013

Biotechnol Biofuels

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“…In all variants, the mass yield of mycelium from the glycerol consumed (Y X/S ) was in a range of 49.8-54.7%. Lipid accumulation was more sensitive to temperature than mycelium growth; it reached the maximum (22.2% of biomass) at 28 • C and gradually decreased to 11.5-12.2% as the temperature lowered to 20-22 • C. Our results obtained for the glycerol-grown continuous culture of M. alpina NRRL-A-10995 are in agreement with the data reported for the glucose-grown batch culture of M. alpina; the optimum temperature values for fungal growth and lipid synthesis were different and were 25 and 20 • C, respectively [40].…”

Section: Parameterssupporting

confidence: 90%

“…The cultivation of the Mortierella fungi at low temperatures was widely applied for the optimization of AA production [6,45]. A temperature-shift strategy was developed to increase the AA production by glucose-grown M. alpina; a temperature of 25 • C was maintained for the first 108 h of cultivation and then it was switched to 20 • C. As a result, the lipid and AA production increased by 20% and 26%, respectively [40].…”

Section: Parametersmentioning

confidence: 99%

The Effect of pH and Temperature on Arachidonic Acid Production by Glycerol-Grown Mortierella alpina NRRL-A-10995

et al. 2018

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Arachidonic acid (AA) has a wide range of applications in medicine, pharmacology, diet, infant nutrition, and agriculture, due to its unique biological properties. The microbiological processes involved in AA production usually require carbohydrate substrates. In this paper, we propose a method for AA production from glycerol, an inexpensive and renewable carbon substrate that is produced by the fungal strain, Mortierella alpina NRRL-A-10995. Our experimental results showed that the optimum pH values required for fungal growth and the production of lipids and AA were different and depended on the growth phase of the fungus. The AA production was shown to be extremely sensitive to acidic pH values and was completely inhibited at a pH of 3.0. The optimum temperature for AA production was 20-22 • C. Continuous cultivation of M. alpina occurred in a glycerol-containing medium, and growth limitations were implemented through the addition of nitrogen and the selection of optimal conditions (pH 6.0, 20 • C). This ensured that active AA production occurred (25.2% of lipids and 3.1% of biomass), with the product yield from the consumed glycerol being 1.6% by mass and 3.4% by energy.

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“…There has recently been an increased interest in using microorganisms to produce ARA (Ratledge 2004;Eroshin et al 2000). As an alternative source containing a high intracellular content of ARA, the filamentous fungus, Mortierella alpina, has been extensively studied for ARA production at various fermentation scales (Peng et al 2010;Vali et al 2003). The previous studies reported that cell growth, total fatty acid and ARA yields were influenced by many factors, such as cell morphology, carbon and nitrogen sources, mineral supplementation, and the dissolved oxygen concentration (Higashiyama et al 2002;Jang et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Enhanced arachidonic acid production using a bioreactor culture of Mortierella alpina with a combined organic nitrogen source

Ling

Zeng

Chen

et al. 2016

Bioresour. Bioprocess.

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Background:The organic nitrogen source is one of the key factors affecting Mortierella alpina cell growth as well as arachidonic acid (ARA) production. The aim of the present work is to achieve an optimized recipe of organic nitrogen source for ARA production by M. alpina by testing four organic nitrogen sources. Results:In the flasks, the results showed yeast extract or corn steep liquor were the most suitable sole nitrogen sources for biomass and ARA yield. In the bioreactor, a biomass of 17.5 g L −1 and an ARA yield of 2.7 g L −1 were achieved when using sole yeast extract, while cell autolysis was induced when using sole corn steep liquor; When using the combined nitrogen source with corn steep liquor and yeast extract at a 3:7 weight ratio, the biomass and ARA yield were significantly improved to 37 and 7.8 g L −1, respectively. Conclusions:This work evaluated whether using a mix of organic nitrogen sources could improve ARA production when scaling up from a flask to a bioreactor culture. The 3:7 ratio of corn steep liquor to yeast extract was quite favourable for large-scale ARA production, and as a result, this combination has great potential for improving fungal cultures.

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A temperature-shift strategy for efficient arachidonic acid fermentation by Mortierella alpina in batch culture

Cited by 37 publications

References 20 publications

Efficient conversion of biomass into lipids by using the simultaneous saccharification and enhanced lipid production process

Efficient conversion of biomass into lipids by using the simultaneous saccharification and enhanced lipid production process

The Effect of pH and Temperature on Arachidonic Acid Production by Glycerol-Grown Mortierella alpina NRRL-A-10995

Enhanced arachidonic acid production using a bioreactor culture of Mortierella alpina with a combined organic nitrogen source

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