A temperate river estuary is a sink for methanotrophs adapted to extremes of <scp>pH</scp>, temperature and salinity

Sherry, Angela; Osborne, Kate A.; Sidgwick, Frances R.; Gray, Neil; Talbot, Helen M.

doi:10.1111/1758-2229.12359

Cited by 30 publications

(55 citation statements)

References 55 publications

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“…678 8a,d), suggesting the microbiological community was similar during both periods of 679 deposition or during later colonisation. If the former, this is unexpected given the wetter 680 conditions and presumably greater methane cycling (as inferred from hopane  13 C values) in 681 the upper section, although Sherry et al (2016) showed that there was no significant 682 changes in the overall methanotrophic community distribution in sediment slurry incubations 683 under a range of methane concentrations. Nonetheless, the blocky lignite is associated with 684 generally higher proportions of aminopentol (Fig.…”

Section: Variability In Methanotrophic Sources 638mentioning

confidence: 98%

Polyfunctionalised bio- and geohopanoids in the Eocene Cobham Lignite

Talbot

Bischoff

Inglis

et al. 2016

Organic Geochemistry

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25We investigated the bacteriohopanepolyol (BHP) distribution in the Cobham Lignite 26 sequence (SE England) deposited across the Palaeocene-Eocene boundary including part 27 of the Palaeocene-Eocene Thermal Maximum (PETM) as shown previously by a negative 28 carbon isotope excursion (CIE). A variety of BHPs were identified, including the commonly 29 occurring and non-source specific biohopanoid bacteriohopanetetrol (BHT) and 32, anhydroBHT which was the most abundant polyfunctionalised geohopanoid in the majority of 31 samples. BHPs with a terminal amine functionality, diagnostic biomarkers for methanotrophic 32 bacteria were identified throughout the sequence, with similar distributions in both the lower 33 laminated and upper blocky lignite except that 35-aminobacteriohopanepentol (aminopentol) 34 indicative of Type I methanotrophs (gammaproteobacteria) was generally more abundant in 35 the upper section within the CIE. 36 37The diagenetic fate of these compounds is currently poorly constrained, however, we also 38 identified the recently reported N-containing transformation product anhydroaminotriol and 39 several tentatively assigned novel N-containing structures potentially containing ketone 40 functionalities. Although present throughout the section, there is a sharp peak in the 41 occurrence of these novel compounds correlated with the onset of the CIE and highly 42 isotopically depleted hopanes in the upper part of the laminated lignite, both also correlate 43 well with peak abundance of aminopentol. The significant abundance of these compounds 44 suggests that 35-amino BHPs have their own specific diagenetic pathway, potentially 45 providing an alternative method allowing methanotroph activity to be traced in older samples 46 even if the original biohopanoid markers are no longer present. 47At this time we cannot preclude the possibility that some or all of these BHPs have been 48produced by more recent subsurface activity, post deposition of the lignite to date; however, 49 that would not be expected to generate the observed stratigraphic variability and we suggest 50 that unprecedented observations of a range of highly functionalised biohopanoids in 51 samples of this age could significantly extend the window of their known occurrence. 52

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Section: Variability In Methanotrophic Sources 638mentioning

confidence: 98%

Polyfunctionalised bio- and geohopanoids in the Eocene Cobham Lignite

Talbot

Bischoff

Inglis

et al. 2016

Organic Geochemistry

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“…Temporal profiles of methane removal in the early phase of incubations were consistent with the experiments described by Sherry et al . () with rapid removal of all added methane suggesting comparable methanotroph growth. After methane removal, headspace gas compositions diverged markedly with 50°C incubations showing rapid and extensive re‐emergence of methane (Fig.…”

Section: Resultsmentioning

confidence: 97%

“…At 40°C, aminotriol and aminotetrol were not enriched (Fig. A and B) suggesting that the different Methylobacter species selected for (Sherry et al ., ) produced a low abundance of this compound. Likewise, the thermophilic genus Methylocaldum at 50°C (Sherry et al ., and Supporting Information Table S2) did not produce aminotriol and aminotetrol suggesting their production is restricted to lower temperatures.…”

Section: Resultsmentioning

confidence: 98%

“…identified by Sherry et al . (). Little is known about BHPs in Crenothrix , however, a metagenome (NCBI accession number PRJNA336651) for Crenothrix polyspora described by Stoecker et al .…”

Section: Resultsmentioning

confidence: 99%

“…A). The dominant methanotrophs previously identified at these temperatures (Sherry et al ., ) were from the genus Methylobacter suggesting that such species produce significant quantities of aminotriol ( III ) not just in pure cultures (as also observed for the closely related Methylobacter strain BB5.1, Supporting Information Fig. S2c) but within a more physically, chemically and biologically complex sediment setting.…”

Section: Resultsmentioning

confidence: 99%

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Methanotroph‐derived bacteriohopanepolyol signatures as a function of temperature related growth, survival, cell death and preservation in the geological record

Osborne

Gray

Sherry

et al. 2017

Environ Microbiol Rep

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Interpretation of bacteriohopanepolyol (BHP) biomarkers tracing microbiological processes in modern and ancient sediments relies on understanding environmental controls of production and preservation. BHPs from methanotrophs (35-aminoBHPs) were studied in methane-amended aerobic river-sediment incubations at different temperatures. It was found that: (i) With increasing temperature (4°C-40°C) a 10-fold increase in aminopentol (associated with Crenothrix and Methylobacter spp. growth) occurred with only marginal increases in aminotriol and aminotetrol; (ii) A further increase in temperature (50°C) saw selection for the thermophile Methylocaldum and mixtures of aminopentol and C-3 methylated aminopentol, again, with no increase in aminotriol and aminotetrol. (iii) At 30°C, more aminopentol and an aminopentol isomer and unsaturated aminopentol were produced after methanotroph growth and the onset of substrate starvation/oxygen depletion. (iv) At 50°C, aminopentol and C-3 methylated aminopentol, only accumulated during growth but were clearly resistant to remineralization despite cell death. These results have profound implications for the interpretation of aminoBHP distributions and abundances in modern and past environments. For instance, a temperature regulation of aminopentol production but not aminotetrol or aminotriol is consistent with and, corroborative of, observed aminopentol sensitivity to climate warming recorded in a stratigraphic sequence deposited during the Paleocene-Eocene thermal maximum (PETM).

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Elevated and atmospheric‐level methane consumption by soil methanotrophs of three grasslands in China

et al. 2023

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Background Methane (CH4) oxidation driven by soil aerobic methanotrophs demonstrates the capacity of grassland as a CH4 sink. Methods In this study, we compared the oxidation characteristics of atmospheric‐level and elevated concentration (10%) CH4 in a typical grassland (steppe) on the Loess Plateau, an alpine meadow (meadow) on the Qinghai‐Tibet Plateau, and an inland arid‐area artificial grassland (pasture) in northwest China and investigated the communities of active methanotrophs and their contribution to CH4 oxidation using DNA‐based stable‐isotope probing and Illumina Miseq sequencing. Results The results showed that the oxidation of atmospheric CH4 only occurred in steppe and meadow soils where the USCγ group of methanotrophs was numerically dominant in the methanotroph community. Pasture soils, with their very low relative abundance of USCγ, did not show atmospheric CH4 oxidation. However, a DNA‐stable isotope probing experiment with 10% CH4 indicated that conventional CH4 oxidizers (Methylocaldum and Methylocystis) rather than USCγ communities assimilated significant amounts of 13CH4 for growth. Conclusions The CH4 oxidation mechanisms in the three experimental grassland soils varied significantly. The USCγ group may be obligate oligotrophic microorganisms or their growth requires specific unknown conditions.

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A temperate river estuary is a sink for methanotrophs adapted to extremes of pH, temperature and salinity

Cited by 30 publications

References 55 publications

Polyfunctionalised bio- and geohopanoids in the Eocene Cobham Lignite

Polyfunctionalised bio- and geohopanoids in the Eocene Cobham Lignite

Methanotroph‐derived bacteriohopanepolyol signatures as a function of temperature related growth, survival, cell death and preservation in the geological record

Elevated and atmospheric‐level methane consumption by soil methanotrophs of three grasslands in China

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