A technology for pilot production of bacterial cellulose from oat hulls

Скиба, Е. А.; Будаева, В. В.; Овчинникова, Е. В.; Гладышева, Е. К.; Kashcheyeva, Ekaterina I.; Pavlov, Igor N.; Сакович, Г. В.

doi:10.1016/j.cej.2019.123128

Cited by 62 publications

(23 citation statements)

References 48 publications

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“…Many agricultural and industrial wastes have been already investigated for the BNC production, including cashew tree residues 16 , dry olive mill residues 17 , konjac powder 18 , rice bark 19 , waste beer yeast 20 , oat hulls 21 , and coffee cherry husk 22 . However, there is still substantial interest to find out and utilize economical substrates that could promote the yield of BNC.…”

Section: Resultsmentioning

confidence: 99%

Bacterial nanocellulose from agro-industrial wastes: low-cost and enhanced production by Komagataeibacter saccharivorans MD1

Abol-Fotouh

Hassan

Shokry

et al. 2020

Sci Rep

164

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Bacterial nanocellulose (Bnc) has been drawing enormous attention because of its versatile properties. Herein, we shed light on the BNC production by a novel bacterial isolate (MD1) utilizing various agroindustrial wastes. Using 16S rRNA nucleotide sequences, the isolate was identified as Komagataeibacter saccharivorans MD1. For the first time, BNC synthesis by K. saccharivorans MD1 was investigated utilizing wastes of palm date, fig, and sugarcane molasses along with glucose on the Hestrin-Schramm (HS) medium as a control. After incubation for 168 h, the highest BNC yield was perceived on the molasses medium recording 3.9 g/L with an initial concentration of (v/v) 10%. The physicochemical characteristics of the BNC sheets were inspected adopting field-emission scanning electron microscope (FESEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) analysis. The FESEM characterization revealed no impact of the wastes on either fiber diameter or the branching scheme, whereas the AFM depicted a BNC film with minimal roughness was generated using date wastes. Furthermore, a high crystallinity index was estimated by XRD up to 94% for the date wastes-derived BNC, while the FTIR analyses exhibited very similar profiles for all BNC films. Additionally, mechanical characteristics and water holding capacity of the produced Bncs were studied. Our findings substantiated that expensive substrates could be exchanged by agro-industrial wastes for Bnc production conserving its remarkable physical and microstructural properties. In the last decades, bacterial nanocellulose (BNC) has earned increasing global interest because of its remarkable physical and chemical properties, including green processing, low production costs, elevated mechanical properties, hydrophilicity, excellent biocompatibility, and biodegradability 1,2. Certain gram-negative non-pathogenic bacterial genera like Rhizobium, Xanthococcus, Pseudomonas, Azotobacter, Aerobacter, and Alcaligenes were reported to produce nanocellulose extracellularly, but the most common BNC-producing strains belong to the genus Komagataeibacter (formerly Acetobacter or commonly acetic acid bacteria) 1. Bacteria produce the BNC through a process of dual coupled steps: polymerization and crystallization. In the bacterial cytoplasm, glucose residues polymerize to β-1,4 glucan linear chains where they are extracellularly secreted. The developed chains are crystallized to microfibrils, then certain numbers of microfibrils consolidate to materialize highly pure 3D porous network of entangled nanoribbons of 20-60 nm in width 3 .

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Section: Resultsmentioning

confidence: 99%

Bacterial nanocellulose from agro-industrial wastes: low-cost and enhanced production by Komagataeibacter saccharivorans MD1

Abol-Fotouh

Hassan

Shokry

et al. 2020

Sci Rep

164

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“…The obtained results from our studies on the properties of CN derived from microbial cellulose suggest that new CNs can potentially be used in conventional and high-tech economic sectors. But these efforts can be justified only by scaling up the biosynthesis of BNC with stable characteristics of BNC from worthless non-food plant biomass using a cellulose-producing strain with a high-level adaptation and a high tolerance to contamination [48], which is being planned to be done in the future.…”

Section: Analysis Results For Bnc and Cn Samplesmentioning

confidence: 99%

Bacterial Nanocellulose Nitrates

et al. 2019

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Bacterial nanocellulose (BNC) whose biosynthesis fully conforms to green chemistry principles arouses much interest of specialists in technical chemistry and materials science because of its specific properties, such as nanostructure, purity, thermal stability, reactivity, high crystallinity, etc. The functionalization of the BNC surface remains a priority research area of polymers. The present study was aimed at scaled production of an enlarged BNC sample and at synthesizing cellulose nitrate (CN) therefrom. Cyclic biosynthesis of BNC was run in a semisynthetic glucose medium of 10−72 L in volume by using the Medusomyces gisevii Sa-12 symbiont. The most representative BNC sample weighing 6800 g and having an α-cellulose content of 99% and a polymerization degree of 4000 was nitrated. The nitration of freeze-dried BNC was performed with sulfuric-nitric mixed acid. BNC was examined by scanning electron microscopy (SEM) and infrared spectroscopy (IR), and CN was explored to a fuller extent by SEM, IR, thermogravimetric analysis/differential scanning analysis (TGA/DTA) and 13C nuclear magnetic resonance (NMR) spectroscopy. The three-cycle biosynthesis of BNC with an increasing volume of the nutrient medium from 10 to 72 L was successfully scaled up in nonsterile conditions to afford 9432 g of BNC gel-films. CNs with a nitrogen content of 10.96% and a viscosity of 916 cP were synthesized. It was found by the SEM technique that the CN preserved the 3D reticulate structure of initial BNC fibers a marginal thickening of the nanofibers themselves. Different analytical techniques reliably proved the resultant nitration product to be CN. When dissolved in acetone, the CN was found to form a clear high-viscosity organogel whose further studies will broaden application fields of the modified BNC.

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“…Bacterial cellulose is a natural polymer produced by a class of acetic acid bacteria ( Khan et al, 2020 ; Skiba et al, 2020 ; Ul-Islam et al, 2020b ) and cell-free systems ( Ullah et al, 2015 ). Both the microbial and the cell-free systems utilize different sugar sources and produce β-1,4-glucan chains, which are protruded extracellularly through the pores (i.e., terminal complexes) present in the cellular membrane, and form high ordered structures ( Kim et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Ex situ Synthesis and Characterization of High Strength Multipurpose Bacterial Cellulose-Aloe vera Hydrogels

Ul‐Islam

Ahmad

Fatima

et al. 2021

Front. Bioeng. Biotechnol.

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The innate structural and functional properties of bacterial cellulose (BC) have been greatly improved by developing its composites with other materials for its applications in different fields. In the present study, BC-Aloe vera (BCA) gel composite with high tensile strength was ex situ developed and characterized for its potential applications in environmental and medical fields. FE-SEM micrographs showed the impregnation of Aloe vera gel into the fibril network of BC. The dry weight analysis showed the addition of 40 wt.% Aloe vera contents into the BC matrix. The addition of Aloe vera resulted in a 3-fold increase in the mechanical strength of BCA composite. The critical strain or stress concentration points were accurately identified in the composite using a three-dimensional digital image correlation (3D-DIC) system. The BCA composite retained water for an extended period of up to 70 h. The BCA composite effectively adsorbed Cu, Co, Fe, and Zn metals. Moreover, the BCA composite supported the adhesion and proliferation of MC3T3-E1 cells. The findings of this study suggest that the developed BCA composite could find multipurpose applications in different fields.

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A technology for pilot production of bacterial cellulose from oat hulls

Cited by 62 publications

References 48 publications

Bacterial nanocellulose from agro-industrial wastes: low-cost and enhanced production by Komagataeibacter saccharivorans MD1

Bacterial nanocellulose from agro-industrial wastes: low-cost and enhanced production by Komagataeibacter saccharivorans MD1

Bacterial Nanocellulose Nitrates

Ex situ Synthesis and Characterization of High Strength Multipurpose Bacterial Cellulose-Aloe vera Hydrogels

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