A technique to obviate the risk of inadvertent infection of cell cultures with bovine viral diarrhoea virus

Hassan, Ayesha; Scott, G. R.

doi:10.1016/0021-9975(86)90044-7

Cited by 4 publications

(2 citation statements)

References 6 publications

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“…It may also contribute to the severity of other infectious diseases or disease complexes by having an immunosuppressive effect [14]. Contamination of cell culture and foetal bovine serum (FBS) with BVDV is also a wide-spread problem which has serious implications in the laboratory for the isolation and diagnosis of important veterinary viruses from clinical specimens [7]. It can also be found as a contaminating virus in viral vaccines used in cattle [13,14].…”

Section: Introductionmentioning

confidence: 99%

Specific sequence amplification of bovine viral diarrhoea virus nucleic acid

Schroeder

Balassu-Chan

1990

Archives of Virology

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Bovine viral diarrhoea virus (BVDV) causes infection of cattle worldwide and is a common contaminant of cell cultures in the laboratory. Methods of diagnosis for BVDV are time-consuming and inconsistent. We describe the development of an in vitro test based on enzymatic DNA amplification with Thermus aquaticus DNA polymerase of sequences of BVDV cDNA reverse transcribed from viral RNA. Specific sequences were amplified from infected cell cultures and clinical material using laboratory and field strains of BVDV including both cytopathic and non-cytopathic isolates. Both plus and minus strands of viral RNA can act as suitable templates for cDNA synthesis prior to sequence amplification. Internal restriction digest of amplified sequences and the co-amplification of multiple sequences increased the specificity of the reaction. The significance of the technique in relation to the diagnosis and understanding of strain differences is also discussed.

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Section: Introductionmentioning

confidence: 99%

Specific sequence amplification of bovine viral diarrhoea virus nucleic acid

Schroeder

Balassu-Chan

1990

Archives of Virology

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“…9,18 The developing bovine fetus is readily infected by BVDV, which replicates in several fetal cell types. 10,11,25,27 Cell cultures established from infected fetuses are often infected with BVDV when initiated and remain so indefinitely. Because noncytopathic BVDV does not induce a cytopathic effect, infection of cell cultures may not be detected unless the cultures are tested by immunocytochemical procedures.…”

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confidence: 99%

Methods for Detection and Frequency of Contamination of Fetal Calf Serum with Bovine Viral Diarrhea Virus and Antibodies against Bovine Viral Diarrhea Virus

1991

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Abstract. Methods used by the National Animal Disease Center to test fetal calf serum for contamination with bovine viral diarrhea virus (BVDV) and antibodies against BVDV are described. Using those methods, virus was isolated from 332 of 1,608 (20.6%) lots of raw fetal calf serum obtained specifically for the Center and 93 of 190 (49%) lots of commercially available fetal calf serum. Virus neutralization and immunoperoxidase staining tests were used to detect antibodies against BVDV in 224 of the 1,608 (13.9%) lots of raw fetal calf serum. Both BVDV and antibodies against BVDV were detected in 50 lots of raw serum. The molecular specificity of antibodies against BVDV was determined by radioimmunoprecipitation. Lots of fetal calf serum that contained BVDV-specific antibodies that did not neutralize virus were identified.

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A Method to Detect Bovine Viral Diarrhea Virus Contamination in Cell Cultures using Immunoperoxidase Staining

Castro

Stoffregen²,

Brigman³

et al. 1997

J VET Diagn Invest

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Certain tests in veterinary diagnostic laboratories are dependent on the use of cell cultures free of contamination with noncytopathic (nc) bovine viral diarrhea virus (BVDV). Such tests include virus and chlamydia isolations, serum neutralization assays, and the microplate virus isolation technique for detection of BVDV in persistently infected animals. 1,3 Cells infected with ncBVDV appear morphologically

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A technique to obviate the risk of inadvertent infection of cell cultures with bovine viral diarrhoea virus

Cited by 4 publications

References 6 publications

Specific sequence amplification of bovine viral diarrhoea virus nucleic acid

Specific sequence amplification of bovine viral diarrhoea virus nucleic acid

Methods for Detection and Frequency of Contamination of Fetal Calf Serum with Bovine Viral Diarrhea Virus and Antibodies against Bovine Viral Diarrhea Virus

A Method to Detect Bovine Viral Diarrhea Virus Contamination in Cell Cultures using Immunoperoxidase Staining

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