A technique for transcervical intrauterine insemination of ewes

Halbert, Gavin; Dobson, Howard; Walton, J.S.; Buckrell, B.C.

doi:10.1016/0093-691x(90)90061-w

Cited by 56 publications

(40 citation statements)

References 7 publications

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“…The inner lumen adopts a tortuous quality due to the presence of characteristic funnel-shaped, caudally facing rings (Fig. 2) that project into the lumen along the length of the cervix [18,19]. This causes a drastic narrowing of the lumen, which is the most constricted at the first two caudal rings.…”

Section: Anatomical Structure Of the Ovine Cervix As A Major Factor Lmentioning

confidence: 99%

“…As a result, advancing a pipette through the cervix is problematic due to the difficulty in locating the opening of each successive, eccentrically positioned ring, in addition to a very small luminal diameter [18]. There is a great deal of variability in cervical morphological characteristics between breeds and individual ewes, including the length (5-10 cm), number of rings (3-7), ring ostium diameter (1-3 mm), and the distance between rings (3-5 cm) [18][19][20]. It has also been suggested that the number of folds present increases with age and parity [18].…”

Section: Anatomical Structure Of the Ovine Cervix As A Major Factor Lmentioning

confidence: 99%

“…It is a very long and narrow fibrous organ ( Fig. 1), comprised of mainly connective tissue with an outer serosal layer and inner luminal epithelium [18,19]. The inner lumen adopts a tortuous quality due to the presence of characteristic funnel-shaped, caudally facing rings (Fig.…”

Section: Anatomical Structure Of the Ovine Cervix As A Major Factor Lmentioning

confidence: 99%

“…There is a great deal of variability in cervical morphological characteristics between breeds and individual ewes, including the length (5-10 cm), number of rings (3-7), ring ostium diameter (1-3 mm), and the distance between rings (3-5 cm) [18][19][20]. It has also been suggested that the number of folds present increases with age and parity [18]. Therefore, designing a customized AI catheter for sheep has been particularly challenging [21].…”

Section: Anatomical Structure Of the Ovine Cervix As A Major Factor Lmentioning

confidence: 99%

See 3 more Smart Citations

A Review of Advances in Artificial Insemination (AI) and Embryo Transfer (ET) in Sheep, with the Special Reference to Hormonal Induction of Cervical Dilation and its Implications for Controlled Animal Reproduction and Surgical Techniques

Candappa¹,

Bartlewski²

2011

TORSJ

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Artificial insemination (AI) and embryo transfer (ET) play an important role in the genetic improvement of sheep herds. This review primarily describes the methods that have recently been researched and/or adapted to circumvent the problematic anatomy of the ovine cervix, which prevents the widespread application of transcervical AI and ET in this species. A mechanism of cervical ripening at parturition and the endocrine influences on cervical tone are described as a backdrop to understanding the action of various pharmacological agents used to induce cervical dilation. Although several catheters have been designed to penetrate the ovine cervix, and a specific protocol has been developed for TCAI in sheep (Guelph Method), penetration rates and resulting pregnancy/lambing rates are still inconsistent and unpredictable. Hormonal dilation of the cervix may significantly improve the ease with which the ovine cervix can be traversed. Treatment with Cervidil ® , a dinoprostone-containing vaginal insert with a slow release mechanism, currently used to induce labor in women, could significantly improve cervical penetration in ewes without adverse effects on uterine contractility. This mode of drug delivery holds promise to result in the development of a safe method to induce cervical dilation for a broad range of transcervical reproductive manipulations in mammalian species including, but not limited to, AI, ET, embryo and oocyte recovery, treatment of ringwomb and transcervical surgical procedures. However, more studies are needed to evaluate the effects of hormonal methods on cervical morphology/pathomorphology, sperm/embryo transport and viability, and fertility in both cyclic and seasonally anovular ewes.

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Section: Anatomical Structure Of the Ovine Cervix As A Major Factor Lmentioning

confidence: 99%

Section: Anatomical Structure Of the Ovine Cervix As A Major Factor Lmentioning

confidence: 99%

Section: Anatomical Structure Of the Ovine Cervix As A Major Factor Lmentioning

confidence: 99%

Section: Anatomical Structure Of the Ovine Cervix As A Major Factor Lmentioning

confidence: 99%

See 2 more Smart Citations

A Review of Advances in Artificial Insemination (AI) and Embryo Transfer (ET) in Sheep, with the Special Reference to Hormonal Induction of Cervical Dilation and its Implications for Controlled Animal Reproduction and Surgical Techniques

Candappa¹,

Bartlewski²

2011

TORSJ

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“…Laparoscopic artificial insemination technique (LAI) was developed by Killen and Caffery (1982) and Halbert et. al.…”

Section: Introductionmentioning

confidence: 99%

Result of Advanced Techniques for Artificial Insemination in Ewes With Frozen-Thawed Semen

Myagmarsuren

Myagmardulam

Haliunaa

2015

Mong. J. Agric. Sci.

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Fertility and Its Relationship to Motility Characteristics of Spermatozoa in Ewes After Cervical, Transcervical, and Intrauterine Insemination With Frozen‐Thawed Ram Semen

SÁNCHEZ‐PARTIDA,

WINDSOR,

EPPLESTON

et al. 1999

Journal of Andrology

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The fertility of ewes after artificial insemination and the relationship between fertility and motility characteristics assessed by a computerized motility analysis system were examined with ram semen frozen in diluents reported to improve postthaw motility. The percentages of motile and progressive spermatozoa were better when frozen in proline—or glycine betaine‐containing or HEPES‐based, rather than Trisbased, diluents (P < 0.01). The fertility of spermatozoa frozen in diluents containing proline or glycine betaine was slightly reduced, whereas when both compatible solutes were present, the reduction was more pronounced, in comparison with semen frozen in Tris‐ or HEPES‐based diluents (9.5 versus 71.1 and 66.6%; P < 0.01). Fertility of frozen—thawed spermatozoa was higher after laparoscopic insemination than after cervical or transcervical insemination (P < 0.01). Similarly, higher fertility was obtained after cervical insemination with fresh than with frozen‐thawed semen (32.4 versus 11.3%; P < 0.01). Furthermore, loss of embryos was lower after laparoscopic insemination of ewes with semen frozen in a Tris diluent than with semen frozen in proline diluents, in glycine betaine diluents, or in proline‐plus‐glycine betaine diluents (0.0 versus 26.0, 38.5, and 60.0%; P < 0.001). A wide variation in the postthaw percentage of motile (31.6–59.7%) and progressive (22.6–43.1%) spermatozoa and in the fertility of spermatozoa from individual rams was also observed after laparoscopic (29.2–59.7%) or cervical insemination (8.7–30.5%). Postthaw motility results from immediately after thawing and fertility results from experiments where intrauterine insemination was performed with semen frozen in proline‐ or glycine betaine—containing or HEPES‐ or Tris‐based diluents were pooled and subjected to a pairwise correlation procedure. The correlation analysis showed relationships between some of the motility characteristics (P < 0.01), but there were no relationships between the motility characteristics and fertility.

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A technique for transcervical intrauterine insemination of ewes

Cited by 56 publications

References 7 publications

A Review of Advances in Artificial Insemination (AI) and Embryo Transfer (ET) in Sheep, with the Special Reference to Hormonal Induction of Cervical Dilation and its Implications for Controlled Animal Reproduction and Surgical Techniques

A Review of Advances in Artificial Insemination (AI) and Embryo Transfer (ET) in Sheep, with the Special Reference to Hormonal Induction of Cervical Dilation and its Implications for Controlled Animal Reproduction and Surgical Techniques

Result of Advanced Techniques for Artificial Insemination in Ewes With Frozen-Thawed Semen

Fertility and Its Relationship to Motility Characteristics of Spermatozoa in Ewes After Cervical, Transcervical, and Intrauterine Insemination With Frozen‐Thawed Ram Semen

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