A Technique for the Perfusion of Laboratory Animals for the Recovery of Schistosomes

Yolles, Tamarath K.; Moore, Donald V.; DeGiusti, Dominic L.; Meleney, Henry E.

doi:10.2307/3273678

Cited by 119 publications

(48 citation statements)

References 2 publications

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“…All adult schistosomes were collected from the hepatic portal system and mesenteric veins and removed from the liver by perfusion with ice-cold PBS supplemented with heparin (Yolles et al 1947 ;Smithers and Terry, 1965). Schistosomes were incubated in 24-well plates (Costar), placing 2 male and 2 female worms in each well.…”

Section: Drugsmentioning

confidence: 99%

Morphological effects and tegumental alterations induced by mefloquine on schistosomula and adult flukes ofSchistosoma mansoni

2009

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There is a pressing need to develop novel anti-schistosomal drugs, as current treatment relies largely on praziquantel (PZQ). To further strengthen current evidence of the anti-schistosomal properties of mefloquine (MQ), we studied the temporal effect of this compound in vitro and in vivo, and examined alterations on the tegumental surface of schistosomula and adults of S. mansoni by means of scanning electron microscopy (SEM). Schistosomula and adults were each incubated in vitro using MQ over a wide concentration range (1-100 mg/ml). In addition, mice infected with adult S. mansoni were treated with a single oral dose of 400 mg/kg MQ, and worms were recovered 24, 48, 72, 96 and 120 h following treatment. MQ showed a rapid onset of action on schistosomula in vitro ; 100 and 75 mg/ml of MQ killed schistosomula immediately ; the minimal lethal and effective concentrations of MQ on schistosomula after 1 h were 25 and 5 mg/ml, respectively. Adult worms incubated with 100 and 10 mg/ml of MQ were dead after 1 h and 24 h of incubation, respectively. A hepatic shift of adult schistosomes was observed in mice already 24 h after treatment, and 120 h following treatment >98 % of all worms had translocated to the liver. SEM observations revealed extensive tegumental destruction, including blebbing, shrinking and sloughing, particularly following in vitro incubation and on the tegument of female worms.

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Section: Drugsmentioning

confidence: 99%

Morphological effects and tegumental alterations induced by mefloquine on schistosomula and adult flukes ofSchistosoma mansoni

2009

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“…The schistosomes were collected by perfusion (YOLLES et al, 1947) with ice-cold Hanks's balanced salt solution (HBSS) from mesenteric veins and livers, rinsed 3 times with HBSS containing 300 iu/mL penicillin sodium and 300 iu/mL streptomycin and kept in an ice bath. Hamsters infected with S. laasmatobium were also killed by bleeding, 77 d after infection.…”

Section: Infection and Collection Ofschistosomesmentioning

confidence: 99%

Artemether administered together with haemin damages schistosomes in vitro

Shuhua

Chollet

Utzinger

et al. 2001

Transactions of the Royal Society of Tropical Medicine and Hygi

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We conducted experiments in vitro to assess the effect of artemether in combination with haemin on adult Schisrosoma japonicum, S. mansoni and S. haematobium. When schistosomes were maintained in a medium containing artemether at concentrations of 20 p,g/mL or less for 72 h, no apparent effect on the schistosomes was seen, When the medium contained 50 or 100 pg/mL haemin as well as artemether, the schistosomes showed decreased motor activity 2-24 h after exposure, which was followed by the staining of the whole worm body a reddish-yellow colour, dilatation of the intestine, and extensive vesiculation of the tegument. Some of the schistosomes died 24 h after exposure? and almost all died within 48-72 h. When scbistosomes were exposed to the same concentrations of haemm alone, they were stained a light yelfow colout but there was no apparent effect on their survival. Our findings suggest that artemether interacts with haemin to exert a toxic effect on the worms, which might be of importance in the further elucidation of the mechanism of action of aaemether on schistosomes.

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“…Ao fim de 2 horas de exposição, foi determinado o número de cercárias penetrantes, segundo técnica de Magalhães 11 (1969). Na oitava semana de infecção, os animais sobreviventes foram sacrificados por deslocamento cervical e os vermes coletados através da perfusão do sistema porta-hepático, segundo técnica de Yolles e col. 28 (1947).…”

Section: Infecção Dos Camundongosunclassified

Relação entre a patogenicidade de Schistosoma mansoni em camundongos e a susceptibilidade do molusco vetor: I. Infecciosidade das cercárias e carga de vermes

1991

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A Technique for the Perfusion of Laboratory Animals for the Recovery of Schistosomes

Cited by 119 publications

References 2 publications

Morphological effects and tegumental alterations induced by mefloquine on schistosomula and adult flukes ofSchistosoma mansoni

Morphological effects and tegumental alterations induced by mefloquine on schistosomula and adult flukes ofSchistosoma mansoni

Artemether administered together with haemin damages schistosomes in vitro

Relação entre a patogenicidade de Schistosoma mansoni em camundongos e a susceptibilidade do molusco vetor: I. Infecciosidade das cercárias e carga de vermes

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