A Technique for the Investigation of Genetic Variation in Filamentous Gametophytes of Trichomanes (Hymenophyllaceae)

Ji, Junxia; Russell, Stephen J.; Gibby, Mary; Vogel, Johannes; Sheffield, E.; Peng, HuaiZheng

doi:10.2307/1547709

Cited by 3 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared with a previously published technique on DNA amplification from filmy fern gametophytes (Ji et al. 1994), we not only skipped the crude DNA extraction step, but also demonstrated that various treatment designs can improve the amplification success rate.…”

Section: Results and Disscussionmentioning

confidence: 97%

“…Compared with a previously published technique on DNA amplification from filmy fern gametophytes (Ji et al 1994), we not only skipped the crude DNA extraction step, but also demonstrated that various treatment designs can improve the amplification success rate. Moreover, in this study, Tissue-direct PCR worked successfully across diverse fern species and tissues, including gametophytes, young sporophytes, mature sporophytes (Table 1) and even spores (data not shown).…”

Section: Tissue-direct Pcr Combomentioning

confidence: 97%

See 1 more Smart Citation

Tissue‐direct PCR, a rapid and extraction‐free method for barcoding of ferns

et al. 2009

Molecular Ecology Resources

View full text Add to dashboard Cite

Fern gametophytes and young sporophytes often provide too little material for DNA extraction and are particularly difficult to identify to genus. Here we developed an efficient procedure called 'Tissue-direct PCR', in which a slice of fern tissue is mixed with PCR reagents and primers, allowing certain genomic regions to be amplified directly in the thermal cycler. For these diminutive and featureless stages of ferns, Tissue-direct PCR combined with amplifying plant barcodes promises to make the identification of immature ferns easy and rapid. Tissue-direct PCR would also be very helpful for large-scale ecological studies surveying distribution and population structure.

show abstract

Section: Results and Disscussionmentioning

confidence: 97%

Section: Tissue-direct Pcr Combomentioning

confidence: 97%

Tissue‐direct PCR, a rapid and extraction‐free method for barcoding of ferns

et al. 2009

Molecular Ecology Resources

View full text Add to dashboard Cite

show abstract

“…Korpelainen 1996; Schneller et al . 1998) and restriction fragment length polymorphisms (RFLPs) of amplified fragments of the chloroplast trn L UAA intron (Ji et al . 1994; Rumsey et al .…”

Section: Introductionmentioning

confidence: 99%

Diversity, genetic structure and evidence of outcrossing in British populations of the rock fern Adiantum capillus‐veneris using microsatellites

et al. 2001

View full text Add to dashboard Cite

Microsatellites were isolated and a marker system was developed in the fern Adiantum capillus-veneris. Polymorphic markers were then used to study the genetic diversity and structure of populations within the UK and Ireland where this species grows at the northern edge of its range, requiring a specific rock habitat and limited to a few scattered populations. Three dinucleotide loci detected a high level of diversity (23 alleles and 28 multilocus genotypes) across the UK and Ireland, with nearly all variation partitioned among rather than within populations. Of 17 populations represented by multiple samples, all except four were monomorphic. Heterozygosity was detected in three populations, all within Glamorgan, Wales (UK), showing evidence of outcrossing. We make inferences on the factors determining the observed levels and patterns of genetic variation and the possible evolutionary history of the populations.

show abstract

Systematics of the Pteridophytes

Schneckenburger

1999

Progress in Botany

View full text Add to dashboard Cite

A Technique for the Investigation of Genetic Variation in Filamentous Gametophytes of Trichomanes (Hymenophyllaceae)

Cited by 3 publications

References 7 publications

Tissue‐direct PCR, a rapid and extraction‐free method for barcoding of ferns

Tissue‐direct PCR, a rapid and extraction‐free method for barcoding of ferns

Diversity, genetic structure and evidence of outcrossing in British populations of the rock fern Adiantum capillus‐veneris using microsatellites

Systematics of the Pteridophytes

Contact Info

Product

Resources

About