A technique for the flow cytometric analysis of lymphocytes bearing histamine receptors

Osband, ME; Cohen, EB; McCaffrey, RP; Shapiro, H M

doi:10.1182/blood.v56.5.923.bloodjournal565923

Cited by 4 publications

(4 citation statements)

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“…Melmon et al [36] reported that suppressor T cells expressed H 2 -receptor sites on their surface and further work showed that histamine played a pivotal role as a suppressor of cellular immune function [35,37]. Cimetidine has both an immune-modulating and immune-enhancing effect, by its ability to block the H 2 -receptor of the suppressor T cell [38,39]. Cimetidine may have an immune-modulating effect on the bladder mucosa of patients with PBD, suppressing the suppressor T cells.…”

Section: Discussionmentioning

confidence: 99%

Oral cimetidine gives effective symptom relief in painful bladder disease: a prospective, randomized, double‐blind placebo‐controlled trial

2001

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Objective To evaluate the efficacy of oral cimetidine as a treatment for painful bladder disease (PBD, variously described as a ‘symptom complex’ of suprapubic pain, frequency, dysuria and nocturia in the absence of overt urine infection) by assessing symptom relief and histological changes in the bladder wall tissue components, compared with placebo. Patients and methods The study comprised 36 patients with PBD enrolled into a double‐blind clinical study with two treatment arms, i.e. oral cimetidine or placebo, for a 3‐month trial. Patients were asked to complete a symptom questionnaire (maximum score 35), and underwent cystoscopy and bladder biopsy before treatment allocation. On completing treatment the patients were re‐evaluated by the questionnaire and biopsy. The symptom scores and bladder mucosal histology were compared before and after treatment, and the results analysed statistically to assess the efficacy of cimetidine. Results Of the 36 patients recruited, 34 (94%) completed the study. Those receiving cimetidine had a significant improvement in symptoms, with median symptom scores decreasing from 19 to 11 (P < 0.001). Suprapubic pain and nocturia decreased markedly (P = 0.009 and 0.006, respectively). However, histologically the bladder mucosa showed no qualitative change in the glycosaminoglycan layer or basement membrane, or in muscle collagen deposition, in either group. The T cell infiltrate was marginally decreased in the cimetidine group (median 203 before and 193 after) and increased in the placebo group (median 243 and 250, P > 0.3 and > 0.2, respectively). Angiogenesis remained relatively unchanged. The incidence of mast cells and B cells was sporadic in both groups. Conclusions Oral cimetidine is very effective in relieving symptoms in patients with PBD but there is no apparent histological change in the bladder mucosa after treatment; the mechanism of symptom relief remains to be elucidated.

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Section: Discussionmentioning

confidence: 99%

Oral cimetidine gives effective symptom relief in painful bladder disease: a prospective, randomized, double‐blind placebo‐controlled trial

2001

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“…Binding of FHA-HIS was inhibited in the presence of free histamine; but only at 400-100 mmol/l. Equally high concentrations of histamine were used by Osband et a1 (5) and by ourselves (unpublished) for inhibition of the binding of FHA-HIS to lymphocytes. The low efficiency of molecular histamine to compete for the membrane receptors could probably be explained by the advantage of the multiple ligand-receptor binding of histamine-conjugated albumin.…”

Section: Discussionmentioning

confidence: 99%

“…Histamine (Histamine dihydrochloride, Sigma) was conjugated to fluoresceinated human albumin, FHA (Cappel Laboratories) by ECDI (1-ethyl-3-carbodiimide hydrochloride, Sigma), as described by Osband et al (5).…”

Section: Histamine Conjugation To Albuminmentioning

confidence: 99%

Demonstration of Histamine Receptors on Human Platelets by Flow Cytometry

Hallberg

Dohlsten

Baldetorp

1984

Scandinavian Journal of Haematology

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Fluoresceinated human albumin conjugated with histamine (FHA‐HIS) has been used for the demonstration of histamine receptors on human platelets. Such receptors were demonstrated on 40–63% of peripheral blood platelets in 4 healthy donors. The binding of FHA‐HIS was inhibited on 35–79% of the platelets by the histamine H1 receptor antagonists diphenhydramine and clemastine. The histamine H2 receptor antagonist cimetidine blocked the FHA‐HIS binding on 14–37% of the platelets. It is concluded that histamine H1 as well as H2 receptors occur on human platelets but the receptors are not equally distributed in the platelet population.

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“…Fluorescently tagged hormones and other ligands have been used for the study of cell surface receptors for growth factors (146), chemotactic peptides ( l l n ) , insulin (108,146), low-density lipoprotein (57), and histamine (116), among others. In general, attention has been paid to demonstrating the presence of, and quantitating the number of, such receptors.…”

Section: Fluorescent Probes For Measurement Of Extrinsic Parametersmentioning

confidence: 99%

Multistation multiparameter flow cytometry: A critical review and rationale

Shapiro

1983

Cytometry

133

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The capacity for fluorescence excitation by beams of different wavelengths at separate points along the sample stream, and the capacity for computer analysis of multiparameter data thus obtained, are now available in flow cytometer/sorter systems from commercial producers. It is now readily apparent to most experienced users of flow cytometers that such multiparameter analysis offers the most convenient solution to the problem of characterizing subpopulations of cells within a mixed population. The use of multiple beams facilitates resolution of fluorescence signals from several probes within or upon a single cell and widens the range of analytical alternatives available to experimenters. This critical review discusses the history of the instrumentation, the parameters now measurable and the probes used for their measurement, and the methods for data analysis. Required sensitivity and precision are discussed, leading to the conclusion that many of the advantages of multistation, multiparameter flow cytometry can be made available in less complex and less costly instruments using less powerful sources and less elaborate computer hardware than are presently incorporated in commercial apparatus.Key Terms: Flow cytometry, fluorescent dyes, multiparameter analysis, spectrophotome t r y The development of flow cytometric apparatus, and of related methodology for cell preparation and data analysis, has largely occurred in response to the specific needs of researchers in a relatively small number of academic and industrial laboratories. As a result, much of the design of the first generation of instruments was dictated by the particular tasks to which the apparatus was to be applied. T h e Cytofluorograf series of instruments now made by Ortho (Ortho Diagnostics Systems, a division of Johnson & Johnson, Westwood, MA) descends from the rapid cell spectrophotometer built by Kamentsky and his colleagues a t IBM's Watson Research Laboratory and used for studies on the automation of cancer cytology (75,76,84

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A technique for the flow cytometric analysis of lymphocytes bearing histamine receptors

Cited by 4 publications

References 0 publications

Oral cimetidine gives effective symptom relief in painful bladder disease: a prospective, randomized, double‐blind placebo‐controlled trial

Oral cimetidine gives effective symptom relief in painful bladder disease: a prospective, randomized, double‐blind placebo‐controlled trial

Demonstration of Histamine Receptors on Human Platelets by Flow Cytometry

Multistation multiparameter flow cytometry: A critical review and rationale

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