A technique for the determination of the available oxygen in living carp (Cyprinus carpio) muscle

Lucchiari, Pedro Hélio; Feofiloff, E.F; Boscardim, Ana Teresa; Bacila, Metry

doi:10.1016/0300-9629(84)90615-7

Cited by 12 publications

(1 citation statement)

References 23 publications

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“…[13][14][15][16] It has also been used in biological systems in vivo as well as in the determination of metabolic activity of several species of microorganisms. [17,18] …”

Section: Introductionmentioning

confidence: 98%

Toxicity assessment of 2,4-D and MCPA herbicides in primary culture of fish hepatic cells

Salvo

Malucelli

Silva

et al. 2015

Journal of Environmental Science and Health, Part B

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In this study, we used primary cultures of fish hepatic cells as a tool for evaluating the effects of environmental contamination. Primary hepatic cell cultures derived from the subtropical fish Metynnis roosevelti were exposed to different concentrations (0.275, 2.75 and 27.5 μg L(-1)) of the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-chloro-2-methylphenoxyacetic acid (MCPA). Cellular respiratory activity was evaluated by polarography using three substrates: 0.5 M glucose, 0.5 M succinate and 0.5 M α-ketoglutarate. Significant changes were observed in cellular oxygen consumption with 0.5 M α-ketoglutarate. Even at low concentrations, 2,4-D and MCPA were potent uncouplers of oxidative phosphorylation. Primary cultures of M. roosevelti liver cells may provide a useful tool for the evaluation of environmental contaminant effects. A review of regulations regarding permitted concentrations of these herbicides is needed.

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“…[13][14][15][16] It has also been used in biological systems in vivo as well as in the determination of metabolic activity of several species of microorganisms. [17,18] …”

Section: Introductionmentioning

confidence: 98%