A technique for the assessment of the cytotoxic capacity of CD8+ lymphocytes based on phase images

Vyshenskaya, T. V.; Bolotova, A. A.; Василенко, И. А.; Zverzhhovsky, V. D.; Boldyrev, D. V.; Kretushev, Alexander V.; Evdokimov, Alexey A.

doi:10.1134/s0006350916030222

Cited by 2 publications

(1 citation statement)

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“…Цель «диалога» -выявление и максимальная объективизация бесконечно малых изменений морфофункционального состояния живых клеток и тканей при исследовании пространственно-временной динамики субклеточных структур путем регистрации в реальном времени их отклика на изменение внешних условий. В результате применения инновационного подхода на междисциплинарной платформе взаимодействия физиков, математиков, биологов и медиков получены новые фундаментальные данные по установлению причинно-следственных связей между внутриклеточными процессами в детерминированных клеточных структурах с учетом тончайших изменений морфологической, метаболической и функциональной компонент их функционирования в условиях адаптационной нормы, дизадаптации и патологии [13][14][15][16]. Эти разработки убедительно продемонстрировали, что интерференционная микроскопия представляет собой перспективный метод клеточной диагностики на уровне наноразмерных внутриклеточных структур с сохранением их нативных свойств, который может быть востребован при решении широкого круга биомедицинских проблем, связанных с оптимизацией диагностики и лечения.…”

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A dialogue with the cell: diagnostic real-time technology based on laser interferometry

Василенко

Метелин

Ignat’ev³

et al. 2018

Alʹm. klin. med.

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The paper presents a review of current techniques of the interference microscopy of living functioning cells that allow for assessment of the real-time changes of subcellular microstructures with 2D and 3D reconstructions of the images and multifactorial data analysis. It has been shown that new diagnostic information can be obtained within the research methodology of the so-called interactive dialogue with the cell to identify changes in the morphofunctional state of living cells through registration of their realtime response to environmental fluctuations. We describe physical basis of laser interferometry, problems and specifics of interpretation of cell phase pictures. As an illustration to the practical use of the technique in the clinic we present the results of studies on morphofunctional state of peripheral blood platelets in patients with oral malignancies (OM) and various stages of the tumor. Analysis of circulating platelets with a realtime technique of laser interferometry allowed for identification of compensated activation of the platelet compartment of hemostasis in these patients, as well as an association between the severity of cancer and changes of dimensional cell parameters (an increase of the mean diameter and area of circulating platelets by 23.4, 26.8 and 30.3%, and by 30.2, 32.5 and 38.1% in the patients with OM grades II, III and IV, respectively (p < 0,05 for all comparisons)). The necessary condition for a wider implementation of new diagnostic technologies is a critical evaluation of the efficacy of their use in routine practice, identification of their advantages, disadvantages and differences between current and proposed diagnostic platforms.

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A dialogue with the cell: diagnostic real-time technology based on laser interferometry

Василенко

Метелин

Ignat’ev³

et al. 2018

Alʹm. klin. med.

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The potential and prospects of the quantitative phase imaging (QPI) diagnostic technology in obstetrics and gynecology

Лифенко¹,

Popova²,

Топузов³

et al. 2018

Alʹm. klin. med.

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Rationale:An important role of immunological factors in the endometriosis pathophysiology allows for their consideration as diagnostic and prognostic disease markers. A promising approach to the diagnosis of immunological abnormalities is based on the non-invasive cell diagnostic technology of quantitative phase imaging (QPI), with underlying principles of laser interferometry and holography.Aim:To assess the QPI potential to identify diagnostic criteria for cell immunity abnormalities in female patients with external endometriosis.Materials and methods:We performed a quantitative assessment of the cytotoxic potential of peripheral blood lymphocytes from 22 patients with endometrioid ovarian cysts (mean age, 27.1 ± 3.4 years), 20 patients with uterine leiomyoma (mean age, 29.3 ± 4.1 years) and 20 health non-pregnant women of child-bearing age (mean age, 28.7 ± 3.6 years). We used the non-invasive QPI technique with the module for phase interference microscopy of the Russian hardand software complex Bioni (Westtrade Ltd., Russia).Results:We studied phase interference pictures of native lymphocytes to identify morphological and densitometry characteristics for CD4+ и CD8+ cells. Two groups of cells were identified in the cytotoxic lymphocyte population, which differed in their values of the phase thickness (Hcyt) and the area of cytoplasm adjacent to the membrane and containing the perforin granules (Scyt). It was found, that in the healthy controls the proportion of perforin-positive cytotoxic CD8+ lymphocytes was 37.1 ± 5.15%, in the patients with endometriosis 29.8 ± 6.34%, and in those with uterine leiomyoma 42.6 ± 5.89%.Discussion:Assessment of the lymphocyte cytotoxic potential by quantitative determination of cells containing perforin granules may be an important diagnostic and prognostic criterion of the disease course.Conclusion:Studies of the intracellular structures of lymphocytes by non-invasive and non-reagent technology QPI allows not only for a detailed evaluation of pathophysiological mechanisms of immune competent cell transformation in the course of a pathological process, but also gives future directions for development of new approaches to functional assessment of the immune system based on the results obtained.

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A technique for the assessment of the cytotoxic capacity of CD8+ lymphocytes based on phase images

Cited by 2 publications

References 13 publications

A dialogue with the cell: diagnostic real-time technology based on laser interferometry

A dialogue with the cell: diagnostic real-time technology based on laser interferometry

The potential and prospects of the quantitative phase imaging (QPI) diagnostic technology in obstetrics and gynecology

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