A technique for oral exfoliative cytology

Background:Squamous cell carcinoma (SCC) is the third most common cause of oral morbidity in India despite the numerous advances made in the treatment protocol.Aim:To compare the cytomorphometric changes of oral mucosal cells in normal subjects (Group I) with that of tobacco users without any lesion (Group II), tobacco users with oral leukoplakia (Group III), and tobacco users with oral SCC (Group IV) through a semi-automated image analysis system.Materials and Methods:Oral mucosal cells collected from study subjects (n = 100) stained using rapid Papanicolaou stain. Photomicrograph of 50 nonoverlapping cells captured at 50× magnification with a digital image capture system. Cytomorphometric analysis of cells in the captured images was performed with Image-Pro image analysis software. Image analysis was performed to obtain cell diameter (CD), cytoplasmic area (CyA), nuclear diameter (ND), nuclear area (NA), and nuclear-to-cytoplasmic ratio. These values were statistically compared among the groups using one-way analysis of variance (ANOVA) and Mann-Whitney U test.Results:The ND, NA, and nuclear-to-cytoplasmic ratio values were found to be increased in the samples collected from leukoplakia and oral SCC. The CD and CyA decreased compared to the normal mucosa in oral SCC samples.Conclusion:The cytomorphometric changes observed in samples from oral SCC and oral leukoplakia were consistent with the current diagnostic features. Hence, the semi-automated cytomorphometric analysis of oral mucosal cells can be used as an objective adjunct diagnostic tool in the diagnosis of these lesions.

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“…[ 4 5 ] Among oral cancers, squamous cell carcinoma (SCC) comprises 90% of all oral malignancies. [ 6 7 ]…”

Section: Introductionmentioning

confidence: 99%

Comparative cytomorphometric analysis of oral mucosal cells in normal, tobacco users, oral leukoplakia and oral squamous cell carcinoma

Sunil

Ravindran

Anilkumar

2015

J Cytol

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“…Since then, exfoliative cytology has gained a widespread use, not only confined to gynecologic endocrinology but also to other various departments including that of oral cancer detection and in early diagnosis of Acquired Immunodeficiency Syndrome (AIDS). [5][6][7][8] Many techniques have been employed for collecting the exfoliated cells of the oral mucosa like oral rinse technique, scraping the oral mucosa by means of a steel spatula, wooden tongue depressor, and oral CDX brushes 9 . In the present study, wooden tongue depressor was used as it is easy to use, disposable, leads to good amount of exfoliated sample collection and even distribution of obtained sample onto a glass slide.…”

Section: Discussionmentioning

confidence: 99%

“…[2][3][4] These changes can be observed microscopically by performing a simple procedure known as exfoliative cytology, where the irradiated and exfoliated oral mucosal cells are procured, stained and viewed under microscope to study the cellular and nuclear alterations within the cell. 5,6 Hence, the present study was done to evaluate the cytomorphometric changes in the oral (buccal mucosa) mucosal cells following exposure to three dimensional projections like computed tomography.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Cytomorphometric Changes Observed in the Oral Mucosal Cells Exposed to Computed Tomography

Srii

2018

J Coll Med Sci-Nepal

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Background:Radiography forms an important as well as an integral part of diagnosis which is used in various fields of medical and dental services to give an appropriate diagnosis. But, diagnostic radiations also induce some amount of damage to the cell at cytogenetic levels, depending on the dosage of the radiation administered. Objective:The objective of the present study was to observe the cytomorphometric changes in the buccal mucosa of patients who were exposed to computed tomography.Materials and methods:Study sample consisted of 15 patients who were exposed to Computed Tomography prior to treatment planning for placement of dental implants. Buccal mucosal smears were obtained pre and 10 days post radiation exposure by using a wooden tongue depressor and the contents were smeared on to clean glass slides and fixed using Biofix spray. Post fixation, these smears were stained using rapid PAP stain and viewed under compound microscope. Cytomorphometric analysis was performed using image ‘J’ software for 50 cells. Statistical analysis was done using SPSS software.Results: Cytomorphometric analysis of the buccal mucosal cells, did show variations in the nuclear and cellular parameters, but statistical significant results were obtained in case of nuclear diameter and nuclear area only suggesting genotoxic alterations following exposure to computed tomography.Conclusion: Oral buccal mucosal cells showed significant cytomorphometric changes in the nuclear parameters after exposure to X-rays during Computed Tomography. A longitudinal study consisting of a bigger sample size is required to evaluate the long-term effects of radiation on these buccal mucosa cells.Keywords: buccal mucosa; computed tomography; cytomorphometry; radiation.

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“…In Asia, biting tobacco causes a high frequency of oral malignancies and in the US there have been reports of oral snuff as hazard in oral cancer 6 . Squamous cell carcinoma (SCC) comprises 90% of all oral malignancies,among oral cancers [7][8] . Numerous oral SCC arises from premalignant states of the oral cavity [9][10] .…”

Section: Introductionmentioning

confidence: 99%

Assessment of Nuclear Changes between Hans and Arecanut Chewing Patient in a Subset of Population

2020

IJPHRD

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Aim:To study nuclear changes occurring in patients with Arecanut and Hans chewing habits Background: Adverse oral habits lead to premalignant lesions. Tobacco chewing, smoking and alcohol intake have been attributed to as major risk factors.Different forms of tobacco usage are prevalent in India and many of them are specific to certain areas. Hans is mixture of tobacco products usually kept in between the cheek and gum or upper lip teeth and chewing. Arecanut has been recently linked to oral submucosal fibrosis.Exfoliative cytology, which is a quick and simple procedure, is an important alternative to biopsy in certain situations. In exfoliative cytology, cells shed from body surfaces, such as the inside of the mouth, are collected and examined. Materials and Method:Cytological smear was taken among 30 patients using exfoliative cytology method. Tobacco users were grouped into two groups Hans and arecanut chewing habits. Results:The result of the study shows increase in frequency of micronuclei among arecanut users when compared with Hans users and controls Conclusion:The study reveals early identification of premalignant lesion in patient using arecanut and Hans products when compared to normal individual.

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A technique for oral exfoliative cytology

Cited by 9 publications

References 14 publications

Comparative cytomorphometric analysis of oral mucosal cells in normal, tobacco users, oral leukoplakia and oral squamous cell carcinoma

Comparative cytomorphometric analysis of oral mucosal cells in normal, tobacco users, oral leukoplakia and oral squamous cell carcinoma

Evaluation of the Cytomorphometric Changes Observed in the Oral Mucosal Cells Exposed to Computed Tomography

Assessment of Nuclear Changes between Hans and Arecanut Chewing Patient in a Subset of Population

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