A technique for identifying polyploid sectors in grass inflorescences

Morgan, W. G.; Meredith, M. R.

doi:10.1007/bf00036872

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2004

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“…Easton (1973) used the modified leaf squash technique for chromosome counting. Morgan & Meredith (1983) developed a technique for identification of polyploid sectors in grass inflorescences. Microscopic chromosome counting is a very reliable method for ploidy determination but it is very time consuming.…”

Section: Introductionmentioning

confidence: 99%

Developing tetraploid perennial ryegrass(Lolium perenneL.) populations

Nair¹

2004

New Zealand Journal of Agricultural Research

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In a breeding programme aimed at developing tetraploids from Australian adapted germplasm, a root-tip squash technique was initially used for ploidy determination. The highest recovery of tetraploids (39.7%) was obtained by treatment of 1-week-old seedlings with 0.1% colchicine concentration. Significant cultivar × colchicine concentration interaction was observed in seedling survival, highlighting the difference in response of the cultivars to colchicine treatment. Subsequently, flow cytometric estimation of the nuclear DNA content in young leaf tissues was used to screen the C 1 and C 2 generations. The speed and the efficiency of this method make it possible to examine a large number of plants. In the C 1 population the percentage of tetraploids, diploids, and aneuploids was 25, 72.7, and 2.3% respectively, while in the C 2 generation the percentage of tetraploids, diploids, and aneuploids was 43, 2, and 55% respectively.

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Section: Introductionmentioning

confidence: 99%

Developing tetraploid perennial ryegrass(Lolium perenneL.) populations

Nair¹

2004

New Zealand Journal of Agricultural Research

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show abstract

A technique for identifying polyploid sectors in grass inflorescences

Cited by 1 publication

References 2 publications

Developing tetraploid perennial ryegrass(Lolium perenneL.) populations

Developing tetraploid perennial ryegrass(Lolium perenneL.) populations

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