A technique for continuous rearing of <i>Neodiprion sertifer</i> (Geoffroy) (Hym., Diprionidae) in the laboratory

Baldassari, N.; Martini, Antonio; Baronio, Piero

doi:10.1046/j.1439-0418.2003.00720.x

Cited by 4 publications

(5 citation statements)

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“…Female individuals chosen for the olfactometer test were 5-10 d-old virgin specimens. In this species, oviposition starts on average on the 5th day after cocoon emergence (Baldassari et al, 2003).…”

Section: Methodsmentioning

confidence: 99%

The Influence of Pine Volatile Compounds on the Olfactory Response by Neodiprion sertifer (Geoffroy) Females

et al. 2010

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Females of the pine sawfly Neodiprion sertifer (Hymenoptera Diprionidae) usually avoid Pinus pinea trees as host plants. In contrast, this sawfly species is highly attracted by P. sylvestris and P. nigra trees. Here, we investigated which pine volatiles might mediate this behavior by in situ sampling experiments and olfactometer laboratory tests. Volatiles emitted from P. pinea, P. sylvestris, and P. nigra foliage were sampled by solid-phase microextraction (SPME). Analysis of these volatiles by coupled gaschromatography/mass spectrometry revealed that the relative amounts of the compounds emitted by the three species were significantly different. A discriminant analysis showed that the amounts of limonene and myrcene significantly contributed to the species-specific volatile patterns. Pinus pinea emitted higher relative amounts of limonene than the other pine species. Pinus sylvestris emitted the highest relative amounts of myrcene. When testing the response of N. sertifer females to these pine terpenoids in an olfactometer bioassay, a low amount of limonene was attractive, while a repellent effect was evident when higher amounts were used. The sawfly females showed no significant olfactory response to myrcene. These data suggest that low relative amounts of limonene have a significant function in attracting N. sertifer females, while high amounts might contribute to avoidance of a tree.

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Section: Methodsmentioning

confidence: 99%

The Influence of Pine Volatile Compounds on the Olfactory Response by Neodiprion sertifer (Geoffroy) Females

et al. 2010

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“…Seasonal dynamics show that the starch concentration of mature foliage peaks at the beginning of the spring and then decreases to approximately 30% of the original level by the beginning of the summer (Ericsson, ). Thus, the early‐season abundance of foliar carbohydrates may assist young N. sertifer larvae to overcome plant defences and acquire fat reserves, which is especially important considering that N. sertifer adults do not feed (Kolomiets et al ., ) and the adult pre‐oviposition period can last up to 2 weeks (Baldassari et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Second‐generation neonates were generated in accordance with the manipulations described by Baldassari et al . (). Briefly, the development of parental eggs and larvae was accelerated first by hatching eggs 2 weeks earlier (27 March) and rearing the larvae at 21 °C and under short‐day photoperiod (8 h) to prevent summer diapause (Sullivan & Wallace, , ).…”

Section: Methodsmentioning

confidence: 97%

“…Pairs of adults that emerged during June 2006 were allowed to mate in confinement, and females were caged in planta with shoots of potted pine trees that also had been phenologically advanced in their development in a greenhouse for 3 months at 20–25 °C (February to June 2006) because females oviposit only in current‐year foliage. Consistent with previous studies (Baldassari et al ., ), egg hatch began 95 days after oviposition and feeding assays with these neonate larvae were conducted in September 2006.…”

Section: Methodsmentioning

confidence: 99%

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Tight insect–host phenological synchrony constrains the life‐history strategy of European pine sawfly

Chorbadjian

Phelan

Herms

2018

Agri and Forest Entomology

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1 The synchronization of insect and plant phenologies may impose ecological constraints on host specialization and the evolution of life histories. 2 European pine sawfly Neodiprion sertifer (Geoffroy) feeds on mature foliage of Scots pine Pinus sylvestris L. during a limited temporal period in early spring and is univoltine despite the continual presence of this resource throughout the growing season. Reciprocal manipulation of host and insect phenologies demonstrated that larval performance was highest in mid-April, with the window of host susceptibility closing rapidly thereafter. 3 As the host phenology advanced, the foliar concentration of nitrogen, amino acids and soluble carbohydrates decreased, whereas that of terpenoids increased. As the foliar quality declined, larvae ate more in an attempt to compensate, although they were less efficient at converting consumed foliage to body mass. 4 The phenological window for larval development began to reopen late in the growing season as the quality of mature foliage started to improve until it once again supported larval development during the next spring. 5 Neodiprion sertifer must synchronize its life cycle with an ephemeral phenological window of host suitability that closes too early and reopens too late to permit a second generation.

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“…The laboratory rearing of Neodiprion sertifer was performed on pine plants according to Baldassari et al (2003).…”

Section: Plant Materials and Insect Rearingmentioning

confidence: 99%

Neodiprion sertifer defoliation causes long-term systemic changes of oxidative enzyme activities in Scots pine needles

Andersone

Samsone

Ievinsh

2009

Arthropod-Plant Interactions

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The aim of the experiments reported here was to study possible long-term effects of Neodiprion sertifer Geoff. (Hymenoptera: Diprionidae) herbivory, or artificial defoliation, on oxidative enzyme activities in Scots pine (Pinus sylvestris L.) needles as a consequence of induced defense responses. During year 1 (the first season), defoliation by N. sertifer, which feeds on previous season's needles, did not result in statistically significant changes in polyphenol oxidase activity in the current year's needles. In contrast, defoliation did lead to increased peroxidase activity in those needles. In the second season (year 2) N. sertifer defoliation of pine seedlings, also defoliated in the previous season either by larvae or artificially, resulted in a decrease of peroxidase activity in the current year's needles. No significant differences between treatments carried out in year 1 were found in year 2 for peroxidase activity in the previous year's needles. However, defoliation in year 1 by N. sertifer resulted in decreased needle consumption, and higher mortality of larvae, in year two. These results indicate the existence of long-term changes in needle oxidative enzyme activities as a consequence of N. sertifer feeding.

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A technique for continuous rearing of Neodiprion sertifer (Geoffroy) (Hym., Diprionidae) in the laboratory

Cited by 4 publications

References 4 publications

The Influence of Pine Volatile Compounds on the Olfactory Response by Neodiprion sertifer (Geoffroy) Females

The Influence of Pine Volatile Compounds on the Olfactory Response by Neodiprion sertifer (Geoffroy) Females

Tight insect–host phenological synchrony constrains the life‐history strategy of European pine sawfly

Neodiprion sertifer defoliation causes long-term systemic changes of oxidative enzyme activities in Scots pine needles

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