A Taxonomic Study of Flavobacteria and Related Gram Negative Yellow Pigmented Rods

Hayes, P. R.

doi:10.1111/j.1365-2672.1977.tb00761.x

Cited by 47 publications

(28 citation statements)

References 32 publications

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“…Indole production was tested for with Kovacs reagent and with Ehrlich reagent, the latter by using method 3 of Cowan (5). Tributyrin hydrolysis was tested for on nutrient agar containing 1% (vol/vol) glycerol tributyrate, as described by Hayes (11). Phosphatase production was determined by method 1 of Cowan (5).…”

Section: Bacteriological Investigationsmentioning

confidence: 99%

Ochrobactrum anthropi gen. nov., sp. nov. from Human Clinical Specimens and Previously Known as Group Vd

Holmes

Popoff

Kiredjian

et al. 1988

International Journal of Systematic Bacteriology

251

147

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In this study we examined the taxonomic relationships of strains variously labeled Achromobacter species biotypes 1 and 2, Achromobacter group A, and Centers for Disease Control (CDC) groups Vd-1 and Vd-2. Previous studies of ribosomal ribonucleic acid cistron similarities placed these organisms on the Brucella ribosomal ribonucleic acid branch of ribosomal ribonucleic acid superfamily IV; their closest neighbors were Brucella, Phyllobacterium, and the Agrobacterium-Rhizobium complex. We performed a numerical taxonomic analysis of 284 phenotypic features (69 conventional tests, 147 API assimilation tests, 68 API ZYM tests) carried out on 95 strains. These organisms comprised 56 strains thought to correspond to CDC group Vd (including 3 strains originally labeled "Pseudomonas arsenoxydans") and 39 strains (included for reference purposes) representing the genera Achromobacter, Agrobacterium, Alcaligenes, Brucella, Mycoplana, Phyllobacterium, and Rhizobium. A phenotypic analysis showed that group Vd bacteria are most similar to Phyllobacterium. However, strains of Group Vd were shown to be distinct by deoxyribonucleic acid (DNA)-DNA hybridization and by several phenotypic tests from Phyllobacterium and other related taxa. The CDC group Vd strains formed essentially a single taxon in the numerical taxonomic analysis of phenotypic characters and as determined by DNA-DNA hybridization. This taxon could be subdivided into three biotypes (biotypes A, C, and D), but none of these corresponded to the two biotypes originally described among the group Vd strains. For CDC group Vd we propose a new genus and new species, Ochrobactrum anthropi; the type strain is strain CIP 82.115 (= CIP 14970 = NCTC 12168 = LMG 3331). 0. anthropi strains are rod shaped, aerobic, gram negative, nonpigmented, and motile by means of peritrichous flagella, produce acid from several carbohydrates, and reduce both nitrate and nitrite. The guanine-plus-cytosine contents of the DNAs of 29 strains ranged from 56 to 59 mol% . Almost all 56 group Vd strains were originally isolated from various human clinical specimens, commonly from blood cultures. Tatum et al. (25) stated that the genus Achromobacter should comprise gram-negative rod-shaped organisms that had peritrichous flagella, were strictly aerobic, showed oxidative metabolism of carbohydrates, produced oxidase, and failed to produce 3-ketolactose.

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Section: Bacteriological Investigationsmentioning

confidence: 99%

Ochrobactrum anthropi gen. nov., sp. nov. from Human Clinical Specimens and Previously Known as Group Vd

Holmes

Popoff

Kiredjian

et al. 1988

International Journal of Systematic Bacteriology

251

147

View full text Add to dashboard Cite

show abstract

“…Several taxonomic investigations described in the review by Shewan & McMeekin (1983) and by others (Callies & Mannheim, 1978 ;Christensen, 1977 ;Hayes, 1977 ; Oyaizu & Komagata, 198 1) revealed unreasonable overlapping of phenotypic and chemotaxonomic characters, such as G + C contents of DNA and cellular fatty acid composition, between the genera Flavobacterium and Cytophaga. Concerning the isoprenoid quinone characters, the species of both genera were characterized by possessing menaquinone 6 (MK-6) or menaquinone 7 (MK-7) as the major quinone (Oyaizu & Komagata, 1981), and the two genera could not be differentiated.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis

Nakagawa¹,

Yamasato²

1993

Journal of General Microbiology

146

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To clarify the intra-and intergeneric relationships of the genus Cytophaga, 16s rRNA sequences and respiratory isoprenoid quinones were determined for the type strains of the 21 validly published species and one isolate in the genus Cytophaga. The sequence analysis revealed extreme heterogeneity of this genus, which diverged into nine distinct lines of descent. Each lineage of Cytophaga was characterized by possessing either menaquinone-6 (MK-6) or MK-7. The MK-6-possessing species were located in the two lineages that were remote from MK-7 species. One of the MK-6 lineages was composed only of terrestrial species and the other only of marine species. Flavobacterium aquatile, the type species of the genus Flavobacterium, was located in the MK-6 terrestrial lineage. The terrestrial Cytophaga species with MK-6 should be transferred to the genus Flavobacterium. The marine facultative anaerobes with MK-7 were located in the bacteroides branch, and possessed signature sequences with features intermediate between the bacteroides and the flavobacteria subdivisions. Cytophaga hutchinsonii, the type species of the genus Cytophaga, had a close relationship only with Cytophaga auvantiaca. The genus Cytophaga should be restricted to these two cellulose-degrading species. The genus Cytophaga is so heterogeneous that it should be divided into several genera and higher taxa in accordance with the phylogenetic relationships.

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“…This description is based primarily on our results (40-42, 45, 61-63) for clinical isolates. However, to avoid a bias towards isolates of clinical origin, the results of Hayes (36,37) are also taken into account because several of Hayes' strains corresponded to species recognized in our laboratory and were isolated from a variety of nonclinical sources. Likewise, we have also included results from Callies and Mannheim (21) on the respiratory quinones.…”

Section: Proposed Rejection Of Flauobacterium Quatile (Frankland and mentioning

confidence: 99%

Proposal That Flavobacterium breve Be Substituted as the Type Species of the Genus in Place of Flavobacterium aquatile and Emended Description of the Genus Flavobacterium: Status of the Named Species of Flavobacterium * Request for an Opinion

Holmes¹,

Owen²

1979

International Journal of Systematic Bacteriology

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A Taxonomic Study of Flavobacteria and Related Gram Negative Yellow Pigmented Rods

Cited by 47 publications

References 32 publications

Ochrobactrum anthropi gen. nov., sp. nov. from Human Clinical Specimens and Previously Known as Group Vd

Ochrobactrum anthropi gen. nov., sp. nov. from Human Clinical Specimens and Previously Known as Group Vd

Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis

Proposal That Flavobacterium breve Be Substituted as the Type Species of the Genus in Place of Flavobacterium aquatile and Emended Description of the Genus Flavobacterium: Status of the Named Species of Flavobacterium * Request for an Opinion

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