A taxonomic revision of Metarhizium based on a phylogenetic analysis of rDNA sequence data

Driver, Felice; Milner, Richard; Trueman, John W. H.

doi:10.1017/s0953756299001756

Cited by 287 publications

(269 citation statements)

References 47 publications

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“…Eight Metarhizium isolates were isolated and initially identified by morphological and microscopic studies (Table 1 and Fig. 1-2) as per conidial shape and size (Driver et al, 2000;Bischoff et al, 2009). Further identification was confirmed through 5.8SrRNA ITS and RPB1 analysis.…”

Section: Resultsmentioning

confidence: 99%

Metarhizium majus and Metarhizium robertsii show enhanced activity against the coleopteran pests Holotricha serrata and Oryctes rhinoceros

Velavan

Sivakumar

Rangeshwaran

et al. 2018

Journal of Biological Control

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Metarhizium majus and Metarhizium robertsii show enhanced activity against the coleopteran pests Holotricha serrata and Oryctes rhinoceros (Article chronicle: Received: 24.08.2017; Revised: 28.09.2017; Accepted: 30.09.2017) ABSTRACT: Studies were conducted to systematically isolate Metarhizium isolates from the insect cadavers and soils of South India.to identify the isolates at species level. Eight Metarhizium -M. robertsii J.F. Bisch., Rehner & Humber sp. nov. (ArMz3R, ArMz3S and ArMz6W), one isolate of M. majus (J.R. Johnst.) J.F. Bisch., Rehner & Humber (VjMz1W) and four isolates of M. anisopliae (WnMz1S, NlMz2S, BgMz2S and DhMz4R). Topical conidial suspensions (TCS) and powder based formulations (PBF) of the eight indigenous isolates of Metarhizium spp. that were isolated from insect cadavers and soils of South India were tested against coleopteran pests Holotricha serrata L. and Oryctes rhinoceros L. that cause serious damage to sugarcane and palm trees respectively. Against H. serrata TCS of M. robertsii (ArMz6W) 50 of 6.893×10 5 cfu/ml and caused 100% mortality against the 3 rd instar larvae in 5 days; PBF elicited an LC 50 of 7.502×10 5 cfu/ml with 96% mortality in 10 days. Against O. rhinoceros TCS (LC 50 of 9.75×10 5 cfu/ml) of M. majus (VjMz1W) caused 90% mortality in 7 days and the PBF (LC 50 of 9.57×10 5 cfu/ml) caused 86% mortality in 14 days.M. robertsii H. serrata and against O. rhinoceros M. majus

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Section: Resultsmentioning

confidence: 99%

Metarhizium majus and Metarhizium robertsii show enhanced activity against the coleopteran pests Holotricha serrata and Oryctes rhinoceros

Velavan

Sivakumar

Rangeshwaran

et al. 2018

Journal of Biological Control

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“…Metarhizium brunneum, Metarhizium robertsii and Metarhizium anisopliae ) have a wide range of insect hosts, whereas others (e.g. Metarhizium acridum, Metarhizium album ) are more specific for certain insects such as orthopterans or hemipterans [4,5]. Recently, significant insights into the molecular mechanisms controlling host selectivity by EPF have been obtained and specificity-related genes have been characterized.…”

Section: Introductionmentioning

confidence: 99%

Highly specific host-pathogen interactions influence Metarhizium brunneum blastospore virulence against Culex quinquefasciatus larvae

et al. 2018

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Entomopathogenic fungi are potential biological control agents of mosquitoes. Our group observed that not all mosquitoes were equally susceptible to fungal infection and observed significant differences in virulence of different spore types. Conidiospores and blastospores were tested against Culex quinquefasciatus larvae. Blastospores are normally considered more virulent than conidia as they form germ tubes and penetrate the host integument more rapidly than conidia. However, when tested against Cx. quinquefasciatus, blastospores were less virulent than conidia. This host-fungus interaction was studied by optical, electron and atomic force microscopy (AFM). Furthermore, host immune responses and specific gene expression were investigated. Metarhizium brunneum (formerly M. anisopliae) ARSEF 4556 blastospores did not readily adhere to Culex larval integument and the main route of infection was through the gut. Adhesion forces between blastospores and Culex cuticle were significantly lower than for other insects. Larvae challenged with blastospores showed enhanced immune responses, with increased levels of phenoloxidase, glutathione-S-transferase, esterase, superoxide dismutase and lipid peroxidase activity. Interestingly, M. brunneum pathogenicity/stress-related genes were all down-regulated in blastospores exposed to Culex. Conversely, when conidia were exposed to Culex, the pathogenicity genes involved in adhesion or cuticle degradation were up-regulated. Delayed host mortality following blastospore infection of Culex was probably due to lower adhesion rates of blastospores to the cuticle and enhanced host immune responses deployed to counter infection. The results here show that subtle differences in host-pathogen interactions can be responsible for significant changes in virulence when comparing mosquito species, having important consequences for biological control strategies and the understanding of pathogenicity processes.

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“…In New Zealand, M. novazealandicum ( M. flavoviride var. novazealandicum ) was reported to be dominant in soil (Driver et al 2000). Although the habitat types of soil samplings should be considered when discussing the dominant species, because some groups of Metarhizium spp.…”

Section: Discussionmentioning

confidence: 99%

Species associations and distributions of soil entomopathogenic fungi Metarhizium spp. in Japan

et al. 2017

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Metarhizium Sorokīn (Hypocreales: Clavicipitaceae) is a genus of facultative parasites of insects found in soils from various environments and is used for pest management. Understanding the habitat selection of Metarhizium spp. is critical to improve the efficacy and persistence of these fungi as microbial insecticides. This study sought to determine the habitat preferences of Metarhizium spp. in Japan. We identified 302 isolates of Metarhizium spp. as eight species by a combination of PCR–RFLP and phylogenetic analysis of DNA sequences. M. pingshaense was the predominant species in Japan and was most frequently isolated from both forest and agricultural environments. On the other hand, M. brunneum and M. pemphigi were comparatively restricted to forest environments. A similar species association was detected in a small area that was intensively investigated, where 7 species including 14 genotypes were isolated from soil. The results of this study have revealed different habitat preferences among Metarhizium spp. in Japan.

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A taxonomic revision of Metarhizium based on a phylogenetic analysis of rDNA sequence data

Cited by 287 publications

References 47 publications

Metarhizium majus and Metarhizium robertsii show enhanced activity against the coleopteran pests Holotricha serrata and Oryctes rhinoceros

Metarhizium majus and Metarhizium robertsii show enhanced activity against the coleopteran pests Holotricha serrata and Oryctes rhinoceros

Highly specific host-pathogen interactions influence Metarhizium brunneum blastospore virulence against Culex quinquefasciatus larvae

Species associations and distributions of soil entomopathogenic fungi Metarhizium spp. in Japan

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