A Taste Circuit that Regulates Ingestion by Integrating Food and Hunger Signals

Yapici, Nilay; Cohn, Raphael; Schusterreiter, Christian; Ruta, Vanessa; Vosshall, Leslie B.

doi:10.1016/j.cell.2016.02.061

Cited by 142 publications

(168 citation statements)

References 86 publications

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“…We next turned our attention to the gustatory system, where relatively little is known about the postsynaptic partners of gustatory receptor neurons (GRNs) (Harris et al ., 2015; Kain and Dahanukar, 2015; Kim et al ., 2017; Miyazaki et al ., 2015; Yapici et al ., 2016). GRNs express gustatory receptors (GRs) that detect non-volatile chemicals and are dispersed in various parts of the body, including the proboscis (labellum and pharynx), tarsal segments of the legs, wing margins, abdomen, intestine and female genitalia (Clyne et al ., 2000; Dunipace et al ., 2001; Scott et al ., 2001; Vosshall and Stocker, 2007).…”

Section: Resultsmentioning

confidence: 99%

“…These neurons mainly inhabited the outer layer of the neighboring AMMC and sent diffuse processes that mostly arborized on the ipsilateral side (Figure 5C). Neurons with similar morphology have been described (Kain and Dahanukar, 2015; Miyazaki et al ., 2015; Yapici et al ., 2016). We also observed clusters of neurons that projected to the SMP, SIP and SLP.…”

Section: Resultsmentioning

confidence: 99%

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Transsynaptic Mapping of Second-Order Taste Neurons in Flies by trans-Tango

Talay

Richman

Snell

et al. 2017

Neuron

280

360

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SUMMARY Mapping neural circuits across defined synapses is essential for understanding brain function. Here we describe trans-Tango, a technique for anterograde transsynaptic circuit tracing and manipulation. At the core of trans-Tango is a synthetic signaling pathway that is introduced into all neurons in the animal. This pathway converts receptor activation at the cell-surface into reporter expression through site-specific proteolysis. Specific labeling is achieved by presenting a tethered ligand at the synapses of genetically defined neurons, thereby activating the pathway in their postsynaptic partners and providing genetic access to these neurons. We first validated trans-Tango in the Drosophila olfactory system and then implemented it in the gustatory system, where projections beyond the first-order receptor neurons are not fully characterized. We identified putative second-order neurons within the sweet circuit that include projection neurons targeting known neuromodulation centers in the brain. These experiments establish trans-Tango as a flexible platform for transsynaptic circuit analysis.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Transsynaptic Mapping of Second-Order Taste Neurons in Flies by trans-Tango

Talay

Richman

Snell

et al. 2017

Neuron

280

360

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show abstract

“…L7-6 neurons, for example, would be predicted to sense aversive compounds based on the presence of their termini at the midline. Analysis of pharyngeal GRN projections also suggests distinct connectivity to higher order neuronal circuits (Yapici et al, 2016). With the molecular tools described here, future investigations of pharyngeal GRNs and pharyngeal taste circuits will provide insight into how internal taste is integrated with external taste to control various aspects of feeding behavior.…”

Section: Discussionmentioning

confidence: 99%

Molecular and Cellular Organization of Taste Neurons in Adult Drosophila Pharynx

Chen

Dahanukar

2017

Cell Reports

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SUMMARY The Drosophila pharyngeal taste organs are poorly characterized despite their location at important sites for monitoring food quality. Functional analysis of pharyngeal neurons has been hindered by the paucity of molecular tools to manipulate them, as well as their relative inaccessibility for neurophysiological investigations. Here, we generate receptor-to-neuron maps of all three pharyngeal taste organs by performing a comprehensive chemoreceptor-GAL4/LexA expression analysis. The organization of pharyngeal neurons reveals similarities and distinctions in receptor repertoires and neuronal groupings compared to external taste neurons. We validate the mapping results by pinpointing a single pharyngeal neuron required for feeding avoidance of L-canavanine. Inducible activation of pharyngeal taste neurons reveals functional differences between external and internal taste neurons and functional subdivision within pharyngeal sweet neurons. Our results provide road maps of pharyngeal taste organs in an insect model system for probing the role of these understudied neurons in controlling feeding behaviors.

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“…The system therefore resolves discrete feeding events, facilitating the study of prandial behavior as well as the finer integration of feeding and spatially derived behaviors such as sleep 4 . In the described setup, the ARC makes measurements from 30 individuals per camera, yet data quality is comparable to that from another recently described capillary-based tool, the Expresso, which is also capable of nanoliter resolution 22 . Replication of an Expresso experiment shows slightly lower absolute feeding in the ARC, but with similar relative changes in consumption across several diets (Supplementary Fig.…”

Section: Introductionmentioning

confidence: 85%

“…n = 20 male flies. Expresso data were extracted from 22 . (b) Replication of Expresso experiment using the ARC.…”

Section: Supplementary Materialsmentioning

confidence: 99%

Simultaneous measurement of sleep and feeding in individual Drosophila

et al. 2017

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Drosophila is widely used for the dissection of genetic and neuronal mechanisms of behavior. Recently, flies have emerged as a model for investigating the regulation of feeding and sleep. Although typically studied in isolation, increasing evidence points to a fundamental connection between these behaviors. Thus, a system for measuring sleep and feeding simultaneously in a single integrated system is important for interpreting behavioral shifts of either state. Here, we describe the construction and use of the Activity Recording CAFE (ARC), a machine-vision based system for the integrated measurement of sleep and feeding in individual Drosophila. Flies feed on liquid food in a microcapillary and consumption is measured by automated tracking of the liquid meniscus over time. Sleep measurements are obtained from positional tracking of the animals and arousal threshold can be determined by vibrational stimulus response. Using this system, a single computer and experimenter can track diverse behaviors from up to 60 individual flies in a single integrated system. The ARC is efficiently assembled with minimal training and each experiment can be run for up to ~7 days, with a total setup and breakdown time of about 2 hours.

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A Taste Circuit that Regulates Ingestion by Integrating Food and Hunger Signals

Cited by 142 publications

References 86 publications

Transsynaptic Mapping of Second-Order Taste Neurons in Flies by trans-Tango

Transsynaptic Mapping of Second-Order Taste Neurons in Flies by trans-Tango

Molecular and Cellular Organization of Taste Neurons in Adult Drosophila Pharynx

Simultaneous measurement of sleep and feeding in individual Drosophila

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