A targeted GC-MS/MS approach for the determination of eight sterols in microgreen and mature plant material

Castellaneta, A.; Losito, Ilario; Leoni, Beniamino; Renna, Massimiliano; Mininni, Carlo; Santamaria, Pietro; Calvano, Cosima Damiana; Cataldi, Tommaso R. I.; Liebisch, Gerhard; Matysik, Silke

doi:10.1016/j.jsbmb.2023.106361

Cited by 2 publications

(14 citation statements)

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“…The growth as microgreens may represent an innovative and alternative use of the most common oleaginous crops, including soybean, sunflower, and rapeseed, along with chia and flax. Consistently with data reported in our previous works, , dealing with glycerophospholipids (GPLs) and sterols, respectively, these ready-to-eat small vegetables might also represent a good source of health-promoting lipids.…”

Section: Introductionsupporting

confidence: 90%

“…Notably, β-sitosterol, campesterol, and brassicasterol were recognized as the primary sterols in rapeseed seedlings. 16 On the other hand, OA and LA were the most abundant FA in this plant material. Based on these observations, SE 28:1/18:1, SE 28:2/18:1, and SE 28:2/18:2 can be reasonably attributed to campesteryl oleate, brassicasteryl oleate, and brassicasteryl linoleate, respectively, while SE 29:1/18:1 and SE 29:1/18:2 are likely corresponding to β-sitosteryl oleate and β-sitosteryl linoleate, respectively.…”

Section: Journal Of Agricultural and Food Chemistry Pubsacsorg/jafc A...mentioning

confidence: 81%

“…A list of the TG sum compositions (fatty acyl chain carbon atoms: fatty acyl chain DB) that were hypothesized on the basis of the results of the GC-MS characterization of FAs for all of the oleaginous microgreens under study is reported in Table . These data were merged with those obtained by GC-MS/MS on sterols in the same microgreen crops . The SEs sum compositions (overall carbon atoms: overall number of DB) accounting for all of the possible fatty acid/sterol combinations are summarized in Table .…”

Section: Resultsmentioning

confidence: 99%

“…The latter more likely corresponds to stigmasteryl linolenate than isofucosteryl linolenate, given the lower abundance of isofucosterol in the sterol profile of soybean. 16 Despite PA's prominence in the FA profile of soybean microgreens, it was not recognized as a major component of SE in this plant material. This observation suggests a prevailing esterification of PA in other lipids such as TGs or GPLs.…”

Section: Characterization Of Se In Oleaginous Microgreensmentioning

confidence: 90%

“…By comparing different solvents, such as methanol, 2-propanol, water, and methanol/chloroform 2:1 (v/v), the most useful homogenization medium was recognized. TGs and SEs were characterized in terms of fatty acyl chain composition using a semi-untargeted approach, in which species corresponding to all of the possible combinations of FAs identified by the preliminary GC-MS analysis and, for SEs, of sterols emerged from the GC-MS/MS characterization of the total (free and esterified) sterol content 16 were considered. Targeted MS/MS analyses were also performed to obtain confirmation.…”

Section: Introductionmentioning

confidence: 99%

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Exploration of the Lipid Profile of Edible Oleaginous Microgreens by Mass Spectrometry-Based Lipidomics

Castellaneta,

Höring,

Losito

et al. 2024

J. Agric. Food Chem.

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The spreading awareness of the health benefits associated with the consumption of plant-based foods is fueling the market of innovative vegetable products, including microgreens, recognized as a promising source of bioactive compounds. To evaluate the potential of oleaginous plant microgreens as a source of bioactive fatty acids, gas chromatography–mass spectrometry was exploited to characterize the total fatty acid content of five microgreens, namely, chia, flax, soy, sunflower, and rapeseed (canola). Chia and flax microgreens appeared as interesting sources of α-linolenic acid (ALA), with total concentrations of 2.6 and 2.9 g/100 g of dried weight (DW), respectively. Based on these amounts, approximately 15% of the ALA daily intake recommended by the European Food Safety Authority can be provided by 100 g of the corresponding fresh products. Flow injection analysis with high-resolution Fourier transform single and tandem mass spectrometry enabled a semi-quantitative profiling of triacylglycerols (TGs) and sterol esters (SEs) in the examined microgreen crops, confirming their role as additional sources of fatty acids like ALA and linoleic acid (LA), along with glycerophospholipids. The highest amounts of TGs and SEs were observed in rapeseed and sunflower microgreens (ca. 50 and 4–5 μmol/g of DW, respectively), followed by flax (ca. 20 and 3 μmol/g DW). TG 54:9, 54:8, and 54:7 prevailed in the case of flax and chia, whereas TG 54:3, 54:4, and 54:5 were the most abundant TGs in the case of rapeseed. β-Sitosteryl linoleate and linolenate were generally prevailing in the SE profiles, although campesteryl oleate, linoleate, and linolenate exhibited a comparable amount in the case of rapeseed microgreens.

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Section: Introductionsupporting

confidence: 90%

Section: Journal Of Agricultural and Food Chemistry Pubsacsorg/jafc A...mentioning

confidence: 81%

Section: Resultsmentioning

confidence: 99%

Section: Characterization Of Se In Oleaginous Microgreensmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

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