A TaqMan real-time PCR assay for Rhizoctonia cerealis and its use in wheat and soil

Woodhall, J. W.; Brown, Matthew; Perkins, K.; Somoza-Valdeolmillos, Eder; Boonham, Neil; Ray, Rumiana V.

doi:10.1007/s10658-016-1083-7

Cited by 10 publications

(3 citation statements)

References 26 publications

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“…solani AG 2‐1, AG 3 (PT), AG 4 (II, II), AG 5, AG 8, and AG 9 (Budge et al, 2009; Woodhall et al, 2013), and R . cerealis (Woodhall et al, 2017). qPCR assays also targeted O .…”

Section: Methodsmentioning

confidence: 99%

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Population dynamics of Rhizoctonia, Oculimacula, and Microdochium species in soil, roots, and stems of English wheat crops

et al. 2020

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This study aimed to elucidate the population dynamics of Rhizoctonia, Oculimacula, and Microdochium species, causing the stem base disease complex of sharp eyespot, eyespot, and brown foot rot in cereals. Pathogen DNA in soil, roots, and stem fractions, and disease expression were quantified in 102 English wheat fields in two seasons. Weather data for each site was collected to determine patterns that correlate with assessed diseases. Oculimacula spp. (66%) and R. solani AG 2-1 (63%) were most frequently detected in soil, followed by R. cerealis (54%) and Microdochium spp. (33%). Oculimacula spp. (89%) and R. cerealis (56%) predominated on roots and soil but were not associated with root rot symptoms, suggesting that these species used soil and roots for survival and as inoculum source. M. nivale was more frequently detected than M. majus on stems up to GS 21-30 and co-occurred on plant samples with O. acuformis. O. yallundae had higher DNA concentration than O. acuformis at the lower 5 cm basal region at GS 37-45. R. cerealis predominated in the upper 15 cm above the base beyond stem extension. Brown foot rot by Microdochium spp. was favoured by cool and wet autumns/winters and dominated in English wheat. Eyespot and sharp eyespot disease index by Oculimacula spp. and R. cerealis, respectively, correlated with wet/humid springs and summers. Results suggested that stem base pathogens generally coexisted; however, their abundance in time and space was influenced by favourable weather patterns and host development, with niche differentiation after stem extension.

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“…solani AG 2‐1, AG 3 (PT), AG 4 (II, II), AG 5, AG 8, and AG 9 (Budge et al, 2009; Woodhall et al, 2013), and R . cerealis (Woodhall et al, 2017). qPCR assays also targeted O .…”

Section: Methodsmentioning

confidence: 99%

“…Targeted Rhizoctonia spp. were R. solani AG 2-1, AG 3 (PT), AG 4 (II, II), AG 5, AG 8, and AG 9 (Budge et al, 2009;Woodhall et al, 2013), and R. cerealis (Woodhall et al, 2017). qPCR assays also targeted O. yallundae, O. acuformis (Walsh et al, 2005), M. nivale, and M. majus (Nielsen et al, 2013).…”

Section: Targeted Qpcr Assaysmentioning

confidence: 99%

Population dynamics of Rhizoctonia, Oculimacula, and Microdochium species in soil, roots, and stems of English wheat crops

et al. 2020

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“…A real-time PCR assay (TaqMan ® ) designed to the rDNA ITS region was used to determine the amount of H. fraxineus DNA(Ioos, Kowalski, Husson, & Holdenrieder, 2009). Quantitative PCR and analysis were carried out as described inWoodhall et al (2017) except Universal Master Mix (Thermo Fisher) was used instead ofEnvironmental Master Mix II. Real-time PCR (TaqMan ® ) was carried out in 96-well plates using the ABI Prism7900HT Sequence Detector System (Applied Biosystems).…”

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Control of Hymenoscyphus fraxineus, the causal agent of ash dieback, using composting

Noble¹,

Woodhall

Dobrovin‐Pennington³

et al. 2019

Forest Pathology

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Viability of Hymenoscyphus fraxineus inocula following temperature treatments for different exposure times was examined in vitro and in aerated flask‐ and large‐scale composting tests using green waste. After an exposure for up to 10 days at 20°C, 97.3% of H. fraxineus mycelium and pseudosclerotia plate cultures remained viable. No viability was detected following a 3‐day exposure to 40°C or a 1‐day exposure to 45°C although pseudosclerotia were more tolerant than mycelium to an exposure to 35°C. Primordial apothecia of H. fraxineus emerged from 62%–100% of infected ash rachises collected from two infected sites and stored at 4°C for 0–5 months; exposure to compost for up to 10 days at 20°C did not affect this emergence. No emergence of H. fraxineus apothecia was observed from ash rachises that were exposed to compost at 45°C for 1 day or at 35°C or 40°C for 3 days in flasks or at 40°C for 1 day or at 30°C for 5 days in a large‐scale composting system. Based on a fitted model, estimates of the survival of H. fraxineus inoculum in infected ash rachises exposed to compost at 50°C for 1 day were 0.081% of that in the untreated H. fraxineus ash rachis inoculum. Increasing loss in viability of H. fraxineus inoculum in infected ash rachises during longer and warmer exposures to compost at 35°C–45°C corresponded with a reduced concentration of pathogen DNA detected in the rachises using real‐time PCR. However, exposure of rachises to compost at >53°C resulted in a smaller reduction in pathogen DNA detected than exposure to compost at lower temperatures, possibly due to the inhibition of enzymatic degradation of DNA at elevated temperatures.

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Pathogenicity and Fungicide Sensitivity of Rhizoctonia solani and R. cerealis Isolates

Kucharska¹,

Katulski²,

Goriewa

et al. 2017

Gesunde Pflanzen

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A TaqMan real-time PCR assay for Rhizoctonia cerealis and its use in wheat and soil

Cited by 10 publications

References 26 publications

Population dynamics of Rhizoctonia, Oculimacula, and Microdochium species in soil, roots, and stems of English wheat crops

Population dynamics of Rhizoctonia, Oculimacula, and Microdochium species in soil, roots, and stems of English wheat crops

Control of Hymenoscyphus fraxineus, the causal agent of ash dieback, using composting

Pathogenicity and Fungicide Sensitivity of Rhizoctonia solani and R. cerealis Isolates

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