A TaqMan-based real-time polymerase chain reaction for the detection of porcine parvovirus

Chen, Hongying; Li, Xiaokang; Bao-an, Cui; Wei, Zhanyong; Li, Xinsheng; Wang, Yanbin; Zhao, Li; Wang, Zhenya

doi:10.1016/j.jviromet.2008.10.029

Cited by 34 publications

(23 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…while 7.5% of samples were co-infected with PCV2 and PPV, but our samples were collected from animals without or with nonspecifi c clinical symptoms. This is in correlation with previous reports of subclinical infection of swine caused by PCV2 [3] and PPV [12]. Our study, as well as fi ndings from previously mentioned studies, showed that PCR method can be used for fast, precise and accurate identifi cation of the aforementioned viruses without isolation in cell line.…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina

et al. 2016

View full text Add to dashboard Cite

The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from non-vaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts of Republic of Srpska, Bosnia and Herzegovina. Porcine circovirus 2 DNA was detected in four lymph node and two spleen samples (15%), while porcine parvovirus DNA was identifi ed in fi ve lymph node samples (12.5%). The presence of both viruses was detected in three lymph node samples (7.5%). Partial nucleotide sequence of ORF1 gene of 2 porcine circovirus 2 and VP2 gene of 2 porcine parvovirus isolates was determined. The nucleotide sequences of two PCV2 isolates from RS-BIH included in phylogenetic typing are similar and cluster together with the strain Mantova isolated from domestic pigs in Italy, strains DE006-14 and DE222-13 isolated from pigs in Germany as well as with the strain Jvnan isolated from pigs in China. Also, analyzed PCV2 isolates were partially similar to the strain NIV-C SRB isolated from pigs in Serbia. The nucleotide sequences of two PPV isolates that were included in phylogenetic typing showed a high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated from pigs in USA and strains 77 and LZ isolated from pigs in China.

show abstract

Section: Discussionsupporting

confidence: 92%

“…Virus isolation in cell cultures is laborious and time consuming, and it cannot be achieved for all PPV strains. Other diagnostic techniques, such as conventional PCR or real -time PCR are widely used [12]. Different studies showed that PCR is very sensitive method for PPV detection [13].…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Since the first occurrence of PPV infection in China was reported in 1982, several PPV strains have been isolated from many Chinese provinces (3, 4). To date, the complete genome sequences of several PPV strains from China are available in GenBank.…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of a Porcine Parvovirus Strain Isolated in Central China

Wang

Chen

et al. 2014

Genome Announc

Self Cite

View full text Add to dashboard Cite

show abstract

“…Yang and Guo have reported the detection of AHV-1 with FQ-PCR method [35,36]. FQ-PCR based on TaqMan™ technology provides certain advantages including high sensitivity, high specificity, and reproducibility, and has been widely used to quantify the copies of viral genomic after optimization [37-44]. In this study, the developed FQ-PCR method was extremely valuable for AHV-1 detection.…”

Section: Introductionmentioning

confidence: 99%

Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe

Zou

Sun

Cheng

et al. 2010

Virol J

View full text Add to dashboard Cite

BackgroundAnatid herpesvirus 1 (AHV-1) is known for the difficulty of monitoring and controlling, because it has a long period of asymptomatic carrier state in waterfowls. Furthermore, as a significant essential agent for viral attachment, release, stability and virulence, gC (UL44) gene and its protein product (glycoprotein C) may play a key role in the epidemiological screening. The objectives of this study were to rapidly, sensitively, quantitatively detect gC gene of AHV-1 and provide the underlying basis for further investigating pcDNA3.1-gC DNA vaccine in infected ducks by TaqMan™ fluorescent quantitative real-time PCR assay (FQ-PCR) with pcDNA3.1-gC plasmid.ResultsThe repeatable and reproducible quantitative assay was established by the standard curve with a wide dynamic range (eight logarithmic units of concentration) and very good correlation values (1.000). This protocol was able to detect as little as 1.0 × 101 DNA copies per reaction and it was highly specific to AHV-1. The TaqMan™ FQ-PCR assay successfully detected the gC gene in tissue samples from pcDNA3.1-gC and AHV-1 attenuated vaccine (AHV-1 Cha) strain inoculated ducks respectively.ConclusionsThe assay offers an attractive method for the detection of AHV-1, the investigation of distribution pattern of AHV-1 in vivo and molecular epidemiological screening. Meanwhile, this method could expedite related AHV-1 and gC DNA vaccine research.

show abstract

A TaqMan-based real-time polymerase chain reaction for the detection of porcine parvovirus

Cited by 34 publications

References 15 publications

Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina

Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina

Complete Genome Sequence of a Porcine Parvovirus Strain Isolated in Central China

Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe

Contact Info

Product

Resources

About