A tandem of ?-tubulin genes preferentially expressed in radicular tissues from Zea mays

Montoliu, Lluı́s; Rigau, Joan; Puigdomènech, Pere

doi:10.1007/bf00015650

Cited by 79 publications

(67 citation statements)

References 49 publications

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“…The predominant expression of TUA1 in roots and inflorescences may be due to high rates of transcription and/or stability of TUA1 mRNA in root tips and young inflorescences. These expression patterns agree with those previously described for ␣-tubulin genes in maize (Montoliu et al, 1989). Maize tubulin genes are primarily expressed in meristematic tissues, such as root tips and coleoptiles, suggesting that their expression is correlated with cell division.…”

Section: Expression Of Tua Cdnassupporting

confidence: 90%

α-Tubulin Missense Mutations Correlate with Antimicrotubule Drug Resistance in Eleusine indica

1998

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Dinitroaniline herbicides are antimicrotubule drugs that bind to tubulins and inhibit polymerization. As a result of repeated application of dinitroaniline herbicides, highly resistant and intermediately resistant biotypes of goosegrass (Eleusine indica) developed in previously wild-type populations. Three ␣ -tubulin cDNA classes (designated TUA1, TUA2, and TUA3) were isolated from each biotype. Nucleotide differences between the susceptible and the resistant (R) ␣ -tubulins were identified in TUA1 and TUA2. The most significant differences were missense mutations that occurred in TUA1 of the R and intermediately resistant (I) biotypes. Such mutations convert Thr-239 to Ile in the R biotype and Met-268 to Thr in the I biotype. These amino acid substitutions alter hydrophobicity; therefore, they may alter the dinitroaniline binding property of the protein. These mutations were correlated with the dinitroaniline response phenotypes (Drp). Plants homozygous for susceptibility possessed the wild-type TUA1 allele; plants homozygous for resistance possessed the mutant tua1 allele; and plants heterozygous for susceptibility possessed both wild-type and mutant alleles. Thus, we conclude that TUA1 is at the Drp locus. Using polymerase chain reaction primer-introduced restriction analysis, we demonstrated that goosegrass genomic DNA can be diagnosed for Drp alleles. Although not direct proof, these results suggest that a mutation in an ␣ -tubulin gene confers resistance to dinitroanilines in goosegrass. INTRODUCTIONGoosegrass ( Eleusine indica ) is considered one of the most troublesome weeds in the world and occurs extensively in croplands of cotton and soybean and in turf in the southeastern United States (Mudge et al., 1984;Vaughn et al., 1990). This weed has been effectively controlled by preemergence application of dinitroaniline herbicides. However, repeated application in monocrop production has "selected" for a highly resistant (R) biotype from previously susceptible (S) biotype populations (Mudge et al., 1984). Although not as common, an intermediately resistant (I) biotype also has been reported (Mudge et al., 1984;Vaughn et al., 1990).Antimitotic dinitroanilines interfere with microtubule assembly, forming a tubulin-dinitroaniline complex that disrupts polymerization and microtubule stability (Morejohn et al., 1987;Hugdahl and Morejohn, 1993). In the unicellular alga Chlamydomonas reinhardtii , dinitroanilines shorten flagella and inhibit flagellar regeneration (Quader and Filner, 1980). In the parasitic protozoa Leishmania mexicana and Trypanosoma brucei , dinitroaniline apparently binds to protozoan tubulins and inhibits proliferation of these parasites (Chan and Fong, 1990;Chan et al., 1993). In higher plants, dinitroanilines work by affecting root growth, especially the development of secondary roots in susceptible plants (Hacskayko and Amato, 1968). In the presence of the herbicide, mitosis in the goosegrass S biotype is arrested at prometaphase, cell plates are misshapen or absent, and cells enlarge isodi...

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Section: Expression Of Tua Cdnassupporting

confidence: 90%

α-Tubulin Missense Mutations Correlate with Antimicrotubule Drug Resistance in Eleusine indica

1998

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“…Developmentally regulated patterns of tubulin transcription have been observed in several plant species, and have been most widely studied in Arabidopsis (Ludwig et al, 1988;Oppenheimer et al, 1988;Carpenter et al, 1992;Chu et al, 1998) and maize (Montoliu et al, 1989(Montoliu et al, , 1990Hussey et al, 1990;Joyce et al, 1992;Villemur et al, 1994;Uribe et al, 1998). Recent in situ hybridization studies in maize indicate transcription of different tubulin genes in distinct groups of differentiating cells (Uribe et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…In Arabidopsis at least six ␣-tubulin genes and nine ␤-tubulin genes are expressed Snustad et al, 1992). There is evidence of at least seven ␣-tubulin genes (Montoliu et al, 1989(Montoliu et al, , 1990(Montoliu et al, , 1992Villemur et al, 1992) and six ␤-tubulin genes (Villemur et al, 1994) expressed in maize. Tissuespecific preferences in accumulation of tubulin transcripts have been reported in both Arabidopsis (Ludwig et al, 1988;Oppenheimer et al, 1988;Carpenter et al, 1992;Snustad et al, 1992) and maize (Joyce et al, 1992;Villemur et al, 1994;Uribe et al, 1998), and in Arabidopsis, specific ␤-tubulin genes were shown to be regulated by light (Leu et al, 1995) and temperature (Chu et al, 1993).…”

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confidence: 99%

Gene-Specific Changes in α-Tubulin Transcript Accumulation in Developing Cotton Fibers

Whittaker

Triplett

1999

Plant Physiology

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The fibers of cotton (Gossypium hirsutum) are single-cell trichomes that undergo rapid and synchronous elongation. Cortical microtubules provide spatial information necessary for the alignment of cellulose microfibrils that confine and regulate cell elongation. We used gene-specific probes to investigate ␣-tubulin transcript levels in elongating cotton fibers. Two discrete patterns of transcript accumulation were observed. Whereas transcripts of ␣-tubulin genes GhTua2/3 and GhTua4 increased in abundance from 10 to 20 d post anthesis (DPA), GhTua1 and GhTua5 transcripts were abundant only through to 14 DPA, and dropped significantly at 16 DPA with the onset of secondary wall synthesis. This is the first report, to our knowledge, of gene-specific changes in tubulin transcript levels during the development of a terminally differentiated plant cell. The decrease in abundance of GhTua1 and GhTua5 transcripts was correlated with pronounced changes in cell wall structure, suggesting that ␣-tubulin isoforms may be functionally distinct in elongating fiber cells. Although total ␣-tubulin transcript levels were much higher in fiber than several other tissues, including the hypocotyl and pollen, none of the ␣-tubulins was specific to fiber cells.

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“…Genes encoding for cytoskeleton elements could control organelle position as well as secretion events related to the establishment of the interface. Tobacco plants containing a construct in which the promoter of a maize ~-tubulin gene was fused with the bacterial GUS gene (Montoliu et a/., 1989(Montoliu et a/., , 1990 show an intense GUS activity in meristematic tissues, including the root meristem (Rigau et aL, 1993). They can be used to examine the effect of mycorrhizal fungi on the transcriptional activation of one foreign ~-tubulin gene.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional activation of a maize α‐tubulin gene in mycorrhizal maize and transgenic tobacco plants

et al. 1996

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SummaryMycorrhizae are symbioses between soil fungi and roots, with substantial modifications of the cells of both partners, Thus, host cells colonized by mycorrhizal fungi may express otherwise inactive genes. Here it is demonstrated that two arbuscular mycorrhizal (AM) fungi, Glomus versiforme and Gigaspora margarita, cause the transcriptional activation of a gene coding for a-tubulin in the colonized cells of maize and transgenic tobacco roots. Tobacco plants transformed with a construct containing the maize TubG3 gene promoter fused upstream from the bacterial GUS gene showed an intense GUS activity only in meristematic regions. When colonized by G. margarita, GUS activity was detected in the cortical root cells containing fungal arbuscules. No GUS activity was found in root cells when transformants carrying TubG 1 promoter were used, nor when Tub~ 3 transformants were colonized by ericoid mycorrhizal fungi, leading to a non-specific interaction. Activation of the TubG 3 appears to be specific to the gene and to the appropriate interaction. Further evidence that fully differentiated host tissues re-activate the TubG 3 gene following colonization by AM fungi also comes from accumulation of the corresponding transcripts in maize root cells containing arbuscules.

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A tandem of ?-tubulin genes preferentially expressed in radicular tissues from Zea mays

Cited by 79 publications

References 49 publications

α-Tubulin Missense Mutations Correlate with Antimicrotubule Drug Resistance in Eleusine indica

α-Tubulin Missense Mutations Correlate with Antimicrotubule Drug Resistance in Eleusine indica

Gene-Specific Changes in α-Tubulin Transcript Accumulation in Developing Cotton Fibers

Transcriptional activation of a maize α‐tubulin gene in mycorrhizal maize and transgenic tobacco plants

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