volume 163, issue 3, P338-343 2017
DOI: 10.1111/eea.12567
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Kacie J. Athey, Eric G. Chapman, James D. Harwood

Abstract: The study of food webs and trophic interactions increasingly relies on PCR-based molecular gutcontent analysis. However, this technique may be prone to error from contamination of minute quantities of DNA; i.e., simply storing specimens together in a liquid medium may lead to cross-contamination. In this study, we used PCR to determine the contamination rate when (1) specimens were stored together in 95% ethanol for various time periods, and (2) predators fall into ethylene glycol-filled pitfall traps where t…

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