A Taenia crassiceps cDNA sequence encoding a putative immunodiagnostic antigen for bovine cysticercosis

Ds, Zarlenga; Ml, Rhoads; Fm, al-Yaman

doi:10.1016/0166-6851(94)00132-4

Cited by 34 publications

(23 citation statements)

References 17 publications

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“…Determining the number of the genes in the family is fundamental for further exploring the expression and regulation of EgAgB in E. granulosus. This will provide insight to more fully understanding its biological function in this and other taeniid species, which share similar gene sequences to those found in E. granulosus [15]–[18].…”

Section: Introductionmentioning

confidence: 90%

The Echinococcus granulosus Antigen B Gene Family Comprises at Least 10 Unique Genes in Five Subclasses Which Are Differentially Expressed

Zhang

Jones

et al. 2010

PLoS Negl Trop Dis

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BackgroundAntigen B (EgAgB) is a major protein produced by the metacestode cyst of Echinococcus granulosus, the causative agent of cystic hydatid disease. This protein has been shown to play an important role in modulating host immune responses, although its precise biological function still remains unknown. It is generally accepted that EgAgB is comprised of a gene family of five subfamilies which are highly polymorphic, but the actual number of genes present is unknown.Methodology/Principal FindingsBased on published sequences for the family, we designed specific primers for each subfamily and used PCR to amplify them from genomic DNA isolated from individual mature adult worms (MAW) taken from an experimentally infected dog in China and individual larval protoscoleces (PSC) excised from a single hydatid cyst taken from an Australian kangaroo. We then used real-time PCR to measure expression of each of the genes comprising the five EgAgB subfamilies in all life-cycle stages including the oncosphere (ONC).Conclusions/SignificanceBased on sequence alignment analysis, we found that the EgAgB gene family comprises at least ten unique genes. Each of the genes was identical in both larval and adult E. granulosus isolates collected from two geographical areas (different continents). DNA alignment comparisons with EgAgB sequences deposited in GenBank databases showed that each gene in the gene family is highly conserved within E. granulosus, which contradicts previous studies claiming significant variation and polymorphism in EgAgB. Quantitative PCR analysis revealed that the genes were differentially expressed in different life-cycle stages of E. granulosus with EgAgB3 expressed predominantly in all stages. These findings are fundamental for determining the expression and the biological function of antigen B.

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Section: Introductionmentioning

confidence: 90%

The Echinococcus granulosus Antigen B Gene Family Comprises at Least 10 Unique Genes in Five Subclasses Which Are Differentially Expressed

Zhang

Jones

et al. 2010

PLoS Negl Trop Dis

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“…AY324065 to AY324085). Two related antigenic proteins from Taenia crassiceps and Taenia solium (Zarlenga, Rhoads and al-Yaman, 1994 ;Chung et al 1999 ;Saghir et al 2000) were used as outgroups. As can be seen in Fig.…”

Section: Nucleotide Sequence Analysis Of Agb Variantsmentioning

confidence: 99%

High polymorphism in genes encoding antigen B from human infecting strains of Echinococcus granulosus

et al. 2005

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S U M M A R YEchinococcus granulosus antigen B (AgB) is encoded by a gene family and is involved in the evasion of the host immune response. E. granulosus exists as a number of strains (G1-G10) that differ in biological characteristics. We used PCR-SSCP followed by DNA sequencing to evaluate sequence variation and transcription profile of AgB in 5 E. granulosus strains#. Twenty-four genomic sequences were isolated and clustered in 3 groups related to 2 of the 5 reported AgB genes. AgB4 genes were present in almost all strains, whereas AgB2 were present as functional genes exclusively in G1/G2 cluster, and as non-functional genes in G5 and the G6/G7 cluster, suggesting inter-strain variation. The AgB transcription patterns, analysed by RT-PCR, showed that AgB2 and AgB4 genes were transcribed in G1, while only the AgB4 gene was transcribed in G7 strain. Cysts from the same strain or cluster shared more genomic and cDNA variants than cysts from different strain or cluster. The level of nucleotide and deduced amino acid sequence variation observed is higher than that reported so far for coding genes of other helminths. Neutrality was rejected for AgB2 genes. These data show the genetic polymorphism of antigen-coding genes among genetically characterized strains of E. granulosus.

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“…Members of the HLBP family of proteins have been identified in Echinococcus granulosus (EgAgB) (Oriol et al, 1971), Taenia solium (TsHLBPs) (Sako et al, 2000; Lee et al, 2007; Kim et al, 2011), Moniezia expansa (MeHLBP) (Jansen and Barrett, 1995; Barrett et al, 1997), Himenolepys diminuta (H-HLBP) (Saghir et al, 2000, 2001), and in Taenia crassiceps (Tc-HLBP) (Zarlenga et al, 1994). Genes sharing high sequence identity with other members of the HLBP family have been identified in Taenia hidatigena (ThLBPs) and Taenia multiceps (TmHLBPs); their sequences have been deposited in GenBank (Wan-zhong et al, 2011).…”

Section: Hlbpsmentioning

confidence: 99%

Lipid binding proteins from parasitic platyhelminthes

Alvite¹,

Esteves²

2012

Front. Physio.

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Two main families of lipid binding proteins have been identified in parasitic Platyhelminthes: hydrophobic ligand binding proteins (HLBPs) and fatty acid binding proteins (FABPs). Members of the former family of proteins are specific to the Cestoda class, while FABPs are conserved across a wide range of animal species. Because Platyhelminthes are unable to synthesize their own lipids, these lipid-binding proteins are important molecules in these organisms. HLBPs are a high molecular mass complex of proteins and lipids. They are composed of subunits of low molecular mass proteins and a wide array of lipid molecules ranging from CoA esters to cholesterol. These proteins are excretory-secretory molecules and are key serological tools for diagnosis of diseases caused by cestodes. FABPs are mainly intracellular proteins of low molecular weight. They are also vaccine candidates. Despite that the knowledge of their function is scarce, the differences in their molecular organization, ligand preferences, intra/extracellular localization, evolution, and phylogenetic distribution, suggest that platyhelminths HLBPs and FABPs should play different functions. FABPs might be involved in the removal of fatty acids from the inner surface of the cell membrane and in their subsequent targeting to specific cellular destinations. In contrast, HLBPs might be involved in fatty acid uptake from the host environment.

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A Taenia crassiceps cDNA sequence encoding a putative immunodiagnostic antigen for bovine cysticercosis

Cited by 34 publications

References 17 publications

The Echinococcus granulosus Antigen B Gene Family Comprises at Least 10 Unique Genes in Five Subclasses Which Are Differentially Expressed

The Echinococcus granulosus Antigen B Gene Family Comprises at Least 10 Unique Genes in Five Subclasses Which Are Differentially Expressed

High polymorphism in genes encoding antigen B from human infecting strains of Echinococcus granulosus

Lipid binding proteins from parasitic platyhelminthes

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