A T-rich nucleic acid-enhanced electrochemical platform based on electroactive silver nanoclusters for miRNA detection

Zhao, Yan; Lu, Changtong; Kong, Weiheng; Zhu, Shuyun; Qu, Fengli

doi:10.1016/j.bios.2022.114215

Cited by 23 publications

(12 citation statements)

References 34 publications

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“…This result was probably because a high volume of the SERS probe for PIK3CA E542K could result in inadequate hybridization between hp3-1 and hp4-1; this may increase background signal, leading to decreased observed signal responses. Furthermore, steric hindrance by the bulky HCR product could hinder recognition and binding, thereby reducing the signal [ 40 , 41 ]. Thus, the volume of the SERS probe for PIK3CA E542K was set to 7 µL.…”

Section: Resultsmentioning

confidence: 99%

A pump-free and high-throughput microfluidic chip for highly sensitive SERS assay of gastric cancer-related circulating tumor DNA via a cascade signal amplification strategy

Cao

Hua

et al. 2022

J Nanobiotechnol

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Circulating tumour DNA (ctDNA) has emerged as an ideal biomarker for the early diagnosis and prognosis of gastric cancer (GC). In this work, a pump-free, high-throughput microfluidic chip coupled with catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) as the signal cascade amplification strategy (CHA–HCR) was developed for surface-enhanced Raman scattering (SERS) assays of PIK3CA E542K and TP53 (two GC-related ctDNAs). The chip consisted of six parallel functional units, enabling the simultaneous analysis of multiple samples. The pump-free design and hydrophilic treatment with polyethylene glycol (PEG) realized the automatic flow of reaction solutions in microchannels, eliminating the dependence on external heavy-duty pumps and significantly improving portability. In the reaction region of the chip, products generated by target-triggered CHA initiated the HCR, forming long nicked double-stranded DNA (dsDNA) on the Au nanobowl (AuNB) array surface, to which numerous SERS probes (Raman reporters and hairpin DNA-modified Cu2O octahedra) were attached. This CHA–HCR strategy generated numerous active “hot spots” around the Cu2O octahedra and AuNB surface, significantly enhancing the SERS signal intensity. Using this chip, an ultralow limit of detection (LOD) for PIK3CA E542K (1.26 aM) and TP53 (2.04 aM) was achieved, and the whole process was completed within 13 min. Finally, a tumour-bearing mouse model was established, and ctDNA levels in mouse serum at different stages were determined. To verify the experimental accuracy, the gold-standard qRT–PCR assay was utilized, and the results showed a high degree of consistency. Thus, this rapid, sensitive and cost-effective SERS microfluidic chip has potential as an ideal detection platform for ctDNA monitoring.

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Section: Resultsmentioning

confidence: 99%

A pump-free and high-throughput microfluidic chip for highly sensitive SERS assay of gastric cancer-related circulating tumor DNA via a cascade signal amplification strategy

Cao

Hua

et al. 2022

J Nanobiotechnol

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“…This method employed a hybrid chain reaction as a signal amplification strategy before the sensing step. The method could detect miRNA-155 as low s 0.39 fM with good LDR from 0.5 fM to 1 nM …”

Section: Metal Ncsmentioning

confidence: 99%

“…Schematic representation of the nanobiosensor for the recognition of miRNA-155 using NAE-AgNCs and HCR. Reproduced with permission from ref . Copyright, 2022, Elsevier.…”

Section: Metal Ncsmentioning

confidence: 99%

Metal Nanoclusters in Point-of-Care Sensing and Biosensing Applications

Nasrollahpour

Jurado‐Sánchez

Moradi

2023

ACS Appl. Nano Mater.

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Nanoclusters are nanostructures consisting of few to tens of atoms which have attracted great interest from the scientific community due to their applications in a myriad of fields, from catalysis to biomedical applications. Due to the discrete energy levels resulting from their ultrasmall size, nanoclusters exhibit distinctly different chemical, optical, and electrical properties compared with their larger nanoparticle counterparts. Unlike previously reported nanostructures with larger dimensions (1–10 nm), which typically have a single advantage (for example, high conductivity or optical properties), nanoclusters can be used as signaling and/or reaction-accelerating materials in almost all sensing assays with enhanced properties. Nanoclusters are among the most promising luminescent emitters (electrochemiluminescent, fluorescent, chemiluminescent, and colorimetry) with high quantum yield and better biocompatibility than conventional emitters. Considering their excelent biocompatibility, tailored modification, and desirable electrochemical and optical tunability, nanoclusters have opened new horizons in designing high-performance sensing and biosensing devices. In addition, they represent excellent electrochemical and photoelectrochemical behaviors with improved functionality and environmentally friendly properties compared to traditional compounds. This review discusses the recent advances in nanoclusters, their applications in sensing and biosensing, current limitations, and prospects to overcome these challenges. The present study gives a much brighter vision of nanoclusters that discusses one by one their particular advantages and role in developing sensing frameworks.

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“…A complementary DNA probe modified with SH can be covalently bonded to the AuNP surface [ 14 , 29 , 41 ], and similarly, the same type of modification can be used for a conjugation on AgNPs [ 18 , 69 ], core–shell Au–Ag NPs [ 26 ], Au–Ag nanocages [ 20 ], AuNP–peptide nanotube composites [ 12 ], Au-coated conglomerates of superparamagnetic NPs [ 43 ], citrate-capped AuNPs electrostatically adsorbed to Fe 3 O 4 NPs [ 70 ], Ag nanoclusters on Au electrode [ 71 ], and hollow Au–Ag nanospheres [ 72 ].…”

Section: Conjugation Strategies Leading To Functionalizationmentioning

confidence: 99%

Formation of miRNA Nanoprobes—Conjugation Approaches Leading to the Functionalization

Vilímová¹,

Hervé-Aubert²,

Chourpa³

2022

Molecules

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Recently, microRNAs (miRNA) captured the interest as novel diagnostic and prognostic biomarkers, with their potential for early indication of numerous pathologies. Since miRNA is a short, non-coding RNA sequence, the sensitivity and selectivity of their detection remain a cornerstone of scientific research. As such, methods based on nanomaterials have emerged in hopes of developing fast and facile approaches. At the core of the detection method based on nanotechnology lie nanoprobes and other functionalized nanomaterials. Since miRNA sensing and detection are generally rooted in the capture of target miRNA with the complementary sequence of oligonucleotides, the sequence needs to be attached to the nanomaterial with a specific conjugation strategy. As each nanomaterial has its unique properties, and each conjugation approach presents its drawbacks and advantages, this review offers a condensed overview of the conjugation approaches in nanomaterial-based miRNA sensing. Starting with a brief recapitulation of specific properties and characteristics of nanomaterials that can be used as a substrate, the focus is then centered on covalent and non-covalent bonding chemistry, leading to the functionalization of the nanomaterials, which are the most commonly used in miRNA sensing methods.

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A T-rich nucleic acid-enhanced electrochemical platform based on electroactive silver nanoclusters for miRNA detection

Cited by 23 publications

References 34 publications

A pump-free and high-throughput microfluidic chip for highly sensitive SERS assay of gastric cancer-related circulating tumor DNA via a cascade signal amplification strategy

A pump-free and high-throughput microfluidic chip for highly sensitive SERS assay of gastric cancer-related circulating tumor DNA via a cascade signal amplification strategy

Metal Nanoclusters in Point-of-Care Sensing and Biosensing Applications

Formation of miRNA Nanoprobes—Conjugation Approaches Leading to the Functionalization

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