A T cell receptor Vβ segment that imparts reactivity to a class II major histocompatibility complex product

Kappler, John W.; Wade, Terri K.; White, Janicé; Kushnir, E; Blackman, Marcia A.; Bill, Jerome; Roehm, Neal W.; Marrack, Philippa

doi:10.1016/0092-8674(87)90567-8

Cited by 385 publications

(175 citation statements)

References 35 publications

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“…The minimum concentration of antibody required was not determined, however. No staining was observed using the anti-T-cell receptor, KJ23 (Kappler et al, 1987) as a control at 50 ng/ml and 50 Ixg/ml. characteristic mobilities on SDS-PAGE under reducing and nonreducing conditions (Parham, 1986) and that they retained binding to the 130-kD polypeptide on immunoblots at concentrations of 1 Ixg/ml, before investigating their effects on kinesin-driven motility (Fig.…”

Section: Effects Of Suk 1-7 On Kinesin-induced Microtubule Translocationmentioning

confidence: 99%

“…The blots in C were probed with 1 lig/ml SUK 1-7 as indicated. The following control antibodies displayed no reaction with the 130-kD kinesin heavy chain; 5 ~tg/ml mouse monoclonal anti-T-cell receptor (KJ 23) (Kappler et al, 1987), 1 lig/ml mouse gamma globulin, and 1 lag/ml each of mouse IgM, mouse IgG1, and mouse IgG2B fractions.…”

Section: Characterization Of Binding Of Suk 1-7 To Sea Urchin Egg Kinmentioning

confidence: 99%

“…We did not obtain antibodies to the 75-kD polypeptides which were present . The KJ23 anti-T-cell receptor antibody (Kappler et al, 1987) was used as a control and gave no significant signal above background. In RIAs with bovine serum albumin or bovine brain tubulin adsorbed to the microtiter plates instead of kinesin, the SUK 1-7 antibodies gave no signal above background (range = 100-300 cpm; see Fig.…”

Section: Characterization Of Binding Of Suk 1-7 To Sea Urchin Egg Kinmentioning

confidence: 99%

“…Gamma counting for bound radioactivity was performed (gamma 300; Beckman Instruments Inc., Palo Alto, CA). The level of nonspecific binding was determined by counting wells coated with BSA (20 gg/ml) or tubulin (20 p.g/ml), and wells coated with kinesin incubated with a nonspecific control antibody KJ23 (Kappler et al, 1987). …”

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Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.

Ingold

Cohn

Scholey

1988

The Journal of Cell Biology

174

153

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Abstract, We have prepared and characterized seven mouse monoclonal antibodies (SUK 1-7) to the 130-kD heavy chain of sea urchin egg kinesin. On immunoblots, SUK 3 and SUK 4 cross-reacted with Drosophila embryo ll6-kD heavy chains, and SUK 4, SUK 5, SUK 6, and SUK 7 bound to the 120-kD heavy chains of bovine brain kinesin. Three out of seven monoclonal antikinesins (SUK 4, SUK 6, and SUK 7) caused a dose-dependent inhibition of sea urchin egg kinesininduced microtubule translocation, whereas the other four monoclonal antibodies had no detectable effect on this motility. The inhibitory monoclonal antibodies (SUK 4, SUK 6, and SUK 7) appear to bind to spatially related sites on an ATP-sensitive microtubule binding 45-kD chymotryptic fragment of the 130-kD heavy chain, whereas SUK 2 binds to a spatially distinct site. None of the monoclonal antikinesins inhibited the microtubule activated MgATPase activity of kinesin, suggesting that SUK 4, SUK 6, and SUK 7 uncouple this MgATPase activity from motility.V ARIOUS forms of intracellular transport are thought to depend upon microtubule-associated mechanochemical ATPases (Vale, 1987). One such enzyme, kinesin (Vale et al., 1985a), binds microtubules in a nucleotide sensitive fashion, and displays a microtubule-activated ATPase activity that drives movement of kinesin-coated objects towards the plus end of microtubules (MTs) ~ in vitro (Vale et al., 1985a, b;Scholey et al., 1985;Brady, 1985; Kuznetsoy and Gelfand, 1986;Porter et al., 1987;Cohn et al., 1987;Saxton et al., 1988;Gelles et al., 1988). Kinesin from a variety of animals contains a major polypeptide with an apparent molecular mass of 110-140 kD, encoded by a single gene in Drosophila (Yang et al., 1988), plus copurifying light chains of Mr = 40-80 kD (Vale et al., 1985a,b;Amos, 1987;Kuznetsov and Gelfand, 1986;Saxton et al., 1988). Bovine brain kinesin is an tt2132 heterotetramer consisting of two 120-kD heavy chains plus two 60-kD light chains, assembled into an elongated molecule (Amos, 1987;Kuznetsov et al., 1988;Bloom et al., 1988). Kinesin is proposed to participate in organelle/vesicle transport, in organizing the endomembrane system, and in mitosis (Vale et al., 1986;Vale, 1987;Leslie et al., 1987;Schroer and Sheetz, 1988), but direct evidence concerning the biological functions of kinesin is currently lacking.Antibodies that inhibit mechanochemical activity have been extremely useful in probing the functions of myosin and 1. Abbreviations used in this paper: MT(s), microtubule(s); PMEG, 0.9 M glycerol, 0.1 M Pipes, pH 6.9, 5 mM EGTA, 2.5 mM MgSO4, 0.5 mM EDTA, 1 mM DTT, 100 Ixg/ml soybean trypsin inhibitor, 1 mg/mlp-tosyl-Larginine methyl ester hydrochloride, 10 Ixg/ml leupeptin, pepstatin, and aprotinin; RT, room temperature. dynein. For example, an antiserum that inhibits the ATPase activity of sea urchin sperm dynein caused a corresponding decrease in the beat frequency of reactivated sea urchin sperm, supporting the hypothesis that the dynein ATPase drives axonemal motility (Ogawa and Mohri, 1975;O...

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Section: Effects Of Suk 1-7 On Kinesin-induced Microtubule Translocationmentioning

confidence: 99%

Section: Characterization Of Binding Of Suk 1-7 To Sea Urchin Egg Kinmentioning

confidence: 99%

Section: Characterization Of Binding Of Suk 1-7 To Sea Urchin Egg Kinmentioning

confidence: 99%

mentioning

confidence: 99%

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Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.

Ingold

Cohn

Scholey

1988

The Journal of Cell Biology

174

153

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“…VB20 was amplified with KgDO (complementary to positions 472-488 of V1520) and XNSC10. V~20-specific PCtLs were done with KguP (positions 241-256 of VB20) and MTB primers, of TCR B transcripts with the MCTBUP (positions [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] and an equimolar mixture of the MTB1DO and MTB2DO primers (complementary to positions 474-493 of C131 and C132), of TCR c~ transcripts with MTCAUP (positions [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] and MTCADO2 primers (complementary to positions 263-279), and of V1317 with MVB17S and MVB17FX, as previously described (10).…”

Section: Methodsmentioning

confidence: 99%

Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice.

Six

Jouvin‐Marche

Loh

et al. 1991

The Journal of Experimental Medicine

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SummaryA cDNA library of TCR/3 chain transcripts from BALB/c thymocytes was constructed using anchored polymerase chain reaction (PCR). Screening of this library led to the identification of a VB gene segment, V/320, structurally related to V/33 and V1317. Genomic analysis of mice displaying deletions in their V/3 loci, together with mapping of cosmid clones, situated V/320 2.5 kb beside VB17. The expression of VB20 was estimated by PCR in mice of different H-2 and Mls types. Peripheral T cells from H-2 k and H-2 d mice did not express V/320, whereas in I-E-negative mice (C57B1/6 and SJL), V/320 transcripts were detected. The lack of V/320 transcripts in (C57B1/6 x CBA/J)F1, (C57B1/6 x BALB/c)F1, and in congenic B6.H-2 k mice suggests that the differential use of V/320 is due to an I-E-mediated donal deletion process. The involvement of the Mls super antigens was excluded by analysis of all Mls type combinations. The nature of the V/B20-deleting element(s) is discussed in the context of the I-EAuperantigen systems controlling the expression of V/311 and VB17.

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Processing and Presentation of Antigen by the Class II Histocompatibility System

Unanue

2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

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A T cell receptor Vβ segment that imparts reactivity to a class II major histocompatibility complex product

Cited by 385 publications

References 35 publications

Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.

Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.

Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice.

Processing and Presentation of Antigen by the Class II Histocompatibility System

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