A systems-level analysis of bile acids effects on rat colon epithelial cells

Yde, Jonathan; Wu, Qi; Borg, Johan; Fenton, Robert A.; Moeller, Hanne B.

doi:10.1152/ajpgi.00178.2021

Cited by 4 publications

(3 citation statements)

References 65 publications

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“…Twenty-three genes included ANKRD40CL, CYP2W1, SLC51B, ISX, CA1, MMP28, CA12, ETNK2, UGDH, RASL12, CRYBG2, CPM, SLC4A4, and VPS13A, which were up-regulated, while CD6, TAP1, and SERPINB2 were down-regulated in both DAH vs. DAN and DAH vs. DLYH. It had been reported that the up-regulation of SLC51B expression inhibited the inflammatory response in the mouse colon, thus protecting against colonic mucosal injury, promoting bile acid transport, and maintaining the healthy homeostasis of the colonic mucosa [38,39]. The Intestine-specific homology cassette (ISX) is a homology cassette-containing protein belonging to a paired subfamily.…”

Section: Discussionmentioning

confidence: 99%

Study on the Characteristics of Coarse Feeding Tolerance of Ding’an Pigs: Phenotypic and Candidate Genes Identification

Song,

Xue,

Wang

et al. 2024

Genes

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Ding’an (DA) pig, a prominent local breed in Hainan Province, exhibits notable advantages in coarse feeding tolerance and high-quality meat. To explore the potential genetic mechanism of coarse feeding tolerance in DA pigs, 60-day-old full sibling pairs of DA and DLY (Duroc-Landrace-Yorkshire) pigs were subjected to fed normal (5%) and high (10%) crude fiber diets for 56 days, respectively. The findings showed that increasing the crude fiber level had no impact on the apparent digestibility of crude fiber, intramuscular fat, and marbling scores in DA pigs, whereas these factors were significantly reduced in DLY pigs (p < 0.05). Through differential expression analysis and Weighted Gene Co-expression Network Analysis (WGCNA) of the colonic mucosal transcriptome data, 65 and 482 candidate genes with coarse feeding tolerance in DA pigs were identified, respectively. Joint analysis screened four key candidate genes, including LDHB, MLC1, LSG1, and ESM1, potentially serving as key regulated genes for coarse feeding tolerance. Functional analysis revealed that the most significant pathway enriched in differential genes associated with coarse feeding tolerance in Ding’an pigs was the signaling receptor binding. The results hold substantial significance for advancing our understanding of the genetic mechanisms governing coarse feeding tolerance in Ding’an pigs.

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Section: Discussionmentioning

confidence: 99%

Study on the Characteristics of Coarse Feeding Tolerance of Ding’an Pigs: Phenotypic and Candidate Genes Identification

Song,

Xue,

Wang

et al. 2024

Genes

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“…Another feeding experiment was carried out by Yde et al 80 In this experiment, rats were fed for 10 days on a diet with added cholic acid, and this diet generated a total fecal bile acid concentration in the colon similar to the high level found in humans with irritable bowel syndrome with diarrhea. First, they found that the diet plus cholic acid appeared to cause activation of the nuclear bile acid receptor farnesoid X receptor (FXR) as well as activation of the membrane bound bile acid receptor TGR5.…”

Section: Progression To Colorectal Cancer: Dna Damage Mutation Epigen...mentioning

confidence: 99%

Bile acids as carcinogens in the colon and at other sites in the gastrointestinal system

Bernstein

2022

Exp Biol Med (Maywood)

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Colon cancer incidence is associated with a high-fat diet. Such a diet is linked to elevated levels of bile acids in the gastrointestinal system and the circulation. Secondary bile acids are produced by microorganisms present at high concentrations in the colon. Recent prospective studies and a retrospective study in humans associate high circulating blood levels of secondary bile acids with increased risk of colon cancer. Feeding mice a diet containing a secondary bile acid, so their feces have the bile acid at a level comparable to that in the feces of humans on a high-fat diet, also causes colon cancer in the mice. Studies using human cells grown in culture illuminate some mechanisms by which bile acids cause cancer. In human cells, bile acids cause oxidative stress leading to oxidative DNA damage. Increased DNA damage increases the occurrence of mutations and epimutations, some of which provide a cellular growth advantage such as apoptosis resistance. Cells with such mutations/epimutations increase by natural selection. Apoptosis, or programmed cell death, is a beneficial process that eliminates cells with unrepaired DNA damage, whereas apoptosis-resistant cells are able to survive DNA damage using inaccurate repair processes. This results in apoptosis-resistant cells having more frequent mutations/epimutations, some of which are carcinogenic. The experiments on cultured human cells have provided a basis for understanding at the molecular level the human studies that recently reported an association of bile acids with colon cancer, and the mouse studies showing directly that bile acids cause colon cancer. Similar, but more limited, findings of an association of dietary bile acids with other cancers of the gastrointestinal system suggest that understanding the role of bile acids in colon carcinogenesis may contribute to understanding carcinogenesis in other organs.

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“…Notably, its effectiveness in alleviating diarrhea has been attributed to the modulation of water movement through the suppression of aquaporin 3 (AQP3) expression in the large intestine [12]. A recent report has also demonstrated that bile acids upregulate the expression of AQP3 in the rat colon [13]. In addition, there are reports indicating that bile acids regulate colonic motility and secretion relating to the development of diarrhea [14,15].…”

Section: Introductionmentioning

confidence: 99%

Goreisan‐induced modulation of fecal bile acid composition and gut microbiota in mice

Watanabe,

Takahashi,

Awale

et al. 2024

Traditional & Kampo Medicine

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AimBile acids are suggested to be involved in the antidiarrhetic effect of Goreisan (GRS). This study aimed to investigate the effect of GRS on fecal bile acid levels and composition of the gut microbiota in mice. Correlation analysis was employed to identify the potential gut microbiota associated with the alterations in fecal bile acid levels induced by GRS.MethodsMale C57BL/6 mice were fed a diet supplemented with GRS water extract for 4 weeks. Feces and cecal contents were collected from the mice to determine bile acid levels and analyze bacterial composition.ResultsGRS administration significantly increased the levels of fecal secondary bile acids, including ω‐muricholic acid (MCA), hyodeoxycholic acid (HDCA), and lithocholic acid (LCA). In contrast, the levels of primary bile acids, such as β‐MCA, cholic acid (CA), and chenodeoxycholic acid (CDCA) showed a marked decrease. Further analysis revealed a positive correlation between the relative abundance of Alloprevotella, Dehalobacterium PAC001221, and Eubacterium g23 and fecal levels of ωMCA, HDCA, and LCA.ConclusionsGRS was suggested to promote the formation of fecal secondary bile acids in mice, possibly through the enhancement of bacterial 7β‐dehydroxylation and 6β‐epimerization activities. These changes may be involved in the antidiarrhetic action of GRS. Concurrent evaluation of fecal bile acids and gut microbiota presents a promising approach for elucidating the novel mechanisms underlying the effects of kampo formulations, particularly in modulating the microbial metabolism of intestinal bile acids.

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A systems-level analysis of bile acids effects on rat colon epithelial cells

Cited by 4 publications

References 65 publications

Study on the Characteristics of Coarse Feeding Tolerance of Ding’an Pigs: Phenotypic and Candidate Genes Identification

Study on the Characteristics of Coarse Feeding Tolerance of Ding’an Pigs: Phenotypic and Candidate Genes Identification

Bile acids as carcinogens in the colon and at other sites in the gastrointestinal system

Goreisan‐induced modulation of fecal bile acid composition and gut microbiota in mice

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