A Systems-Biology Approach to Yeast Actin Cables

Drake, Tyler; Yusuf, Eddy; Vavylonis, Dimitrios

doi:10.1007/978-1-4419-7210-1_19

Cited by 3 publications

(5 citation statements)

References 47 publications

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“…6 ). Actin cables contribute to cell polarization and measurements of their properties in wild type and mutant cells can provide information on the basic biophysical mechanisms of cell growth 37 .…”

Section: Resultsmentioning

confidence: 99%

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SOAX: A software for quantification of 3D biopolymer networks

Vavylonis

Tsai

et al. 2015

Sci Rep

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119

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Filamentous biopolymer networks in cells and tissues are routinely imaged by confocal microscopy. Image analysis methods enable quantitative study of the properties of these curvilinear networks. However, software tools to quantify the geometry and topology of these often dense 3D networks and to localize network junctions are scarce. To fill this gap, we developed a new software tool called “SOAX”, which can accurately extract the centerlines of 3D biopolymer networks and identify network junctions using Stretching Open Active Contours (SOACs). It provides an open-source, user-friendly platform for network centerline extraction, 2D/3D visualization, manual editing and quantitative analysis. We propose a method to quantify the performance of SOAX, which helps determine the optimal extraction parameter values. We quantify several different types of biopolymer networks to demonstrate SOAX's potential to help answer key questions in cell biology and biophysics from a quantitative viewpoint.

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Section: Resultsmentioning

confidence: 99%

“…Fission and budding yeast contain however two types of actin structures in interphase: actin cables and actin patches, both of which are present in images where actin filaments are marked by GFP-CHD, an actin filament side-binding protein 37 . Eliminating the actin patches with the drug CK-666 ( Fig.…”

Section: Resultsmentioning

confidence: 99%

SOAX: A software for quantification of 3D biopolymer networks

Vavylonis

Tsai

et al. 2015

Sci Rep

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119

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“…Actin cables are highly dynamic structures with a halflife of less than a minute Drake et al, 2012). Of the three F-actin structures assembled in yeast, actin cables are relatively less well understood probably because they are highly dynamic and are relatively difficult to image in real time.…”

Section: Actin Cable Dynamicsmentioning

confidence: 99%

“…Also, very little is known about how the assembly, growth, and disassembly of actin cables may be controlled. Although recent studies involving advanced cellular, genomic, and mathematical modeling techniques are beginning to shed light on the assembly and dynamics of actin cables (Martin, ; Kovar et al ., ; Yu et al ., ; Drake et al ., ), a significant amount of work is required to generate a comprehensive understanding of the assembly and dynamics of actin cables.…”

Section: Actin Cables and Cell Polaritymentioning

confidence: 99%

The yeast actin cytoskeleton

2014

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The actin cytoskeleton is a complex network of dynamic polymers, which plays an important role in various fundamental cellular processes, including maintenance of cell shape, polarity, cell division, cell migration, endocytosis, vesicular trafficking, and mechanosensation. Precise spatiotemporal assembly and disassembly of actin structures is regulated by the coordinated activity of about 100 highly conserved accessory proteins, which nucleate, elongate, cross-link, and sever actin filaments. Both in vivo studies in a wide range of organisms from yeast to metazoans and in vitro studies of purified proteins have helped shape the current understanding of actin dynamics and function. Molecular genetics, genome-wide functional analysis, sophisticated real-time imaging, and ultrastructural studies in concert with biochemical analysis have made yeast an attractive model to understand the actin cytoskeleton, its molecular dynamics, and physiological function. Studies of the yeast actin cytoskeleton have contributed substantially in defining the universal mechanism regulating actin assembly and disassembly in eukaryotes. Here, we review some of the important insights generated by the study of actin cytoskeleton in two important yeast models the budding yeast Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe.

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“…Some of the key features of a pandemic are its wide geographic extension, swift disease movement, novelty, severity, high attack rates and explosiveness, minimal population immunity, infectiousness and contagiousness (Gewald 2007). Therefore, a pandemic is categorised as trans-regional and global (Taubenberger & Morens 2009) and has the capacity to threaten all aspects of the economic and social fabric (Drake et al 2012). This study presents the historical background of sports during the Spanish Influenza pandemic.…”

Section: Introductionmentioning

confidence: 99%

Southern Africa Pandemic Management in Sports Observations from the 1918 Influenza and Covid19

Muzorewa¹,

Rwodzi²

2022

The Covid19 Pandemic Impacts of the Coronavirus on Sport and Society

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This chapter evaluates pandemic management systems adopted by southern African sporting communities in responses to the 1918 'Spanish' Influenza and the novel Corona virus pandemic. The two pandemics which occurred in two different historical epochs disturbed sporting activities world-wide, upset social relations, triggered community anxieties, and resulted in shutdown of all sporting activities. This study serves as a litmus test of the current sporting communities in southern Africa to adopt, in unison, effective and timely disaster response mechanisms in order to save human lives from devastating natural phenomena while guaranteeing livelihoods of sports women/men. The chapter argues that sporting policy interventions and institutional frameworks adopted in an endeavour to curb the spread of the pandemic were either weak or ineffective. The argument is based on an analysis of the various dynamics which shaped the pandemic management systems such as racism, class and ad hoc policy (in)consistencies by sporting governments in line with World Health Organisation (WHO) prescriptions. The chapter also has the novelty of exposing the challenges that the poor sporting communities in southern Africa have in procuring resources to survive pandemics. Because Covid-19 is a contemporary phenomenon, the methodological approach is underpinned by a qualitative analysis of data collected through desktop research, government primary documents, virtual interviews and webinar discussions. Both seconddary and primary sources are used to derive data on the 1918 Influenza. After Southern Africa Pandemic Management in Sports 141 a comparative analysis of the two pandemics, the chapter argues that although the pandemics occurred during two astronomically different periods, there have not been many significant changes in pandemic management systems.

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A Systems-Biology Approach to Yeast Actin Cables

Cited by 3 publications

References 47 publications

SOAX: A software for quantification of 3D biopolymer networks

SOAX: A software for quantification of 3D biopolymer networks

The yeast actin cytoskeleton

Southern Africa Pandemic Management in Sports Observations from the 1918 Influenza and Covid19

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