A systems biology approach to dissect the contribution of brassinosteroid and Auxin hormones to vascular patterning in the shoot of<i>Arabidopsis thaliana</i>

Fàbregas, Norma; Ibañes, Marta; Caño‐Delgado, Ana I.

doi:10.4161/psb.5.7.12096

Cited by 14 publications

(7 citation statements)

References 36 publications

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“…Beyond the overall plant dwarfism, detailed phenotypic analysis has permitted the characterization of tissue-specific defects in plant vasculature Ibañes et al, 2009;Fàbregas et al, 2010), pollen development (Ye et al, 2010), root hair patterning (Kuppusamy et al, 2009), and QC activity (González-García et al, 2011). Signal amplification from the BRI1 receptor implies the transcriptional regulation of thousands of genes that, as direct targets of BES1 and BRZ1, may act in different cell types regulating different responses (Sun et al, 2010;Yu et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

The BRASSINOSTEROID INSENSITIVE1–LIKE3 Signalosome Complex Regulates Arabidopsis Root Development

Fàbregas

Boeren

et al. 2013

The Plant Cell

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Brassinosteroid (BR) hormones are primarily perceived at the cell surface by the leucine-rich repeat receptor-like kinase BRASSINOSTEROID INSENSITIVE1 (BRI1). In Arabidopsis thaliana, BRI1 has two close homologs, BRI1-LIKE1 (BRL1) and BRL3, respectively, which are expressed in the vascular tissues and regulate shoot vascular development. Here, we identify novel components of the BRL3 receptor complex in planta by immunoprecipitation and mass spectrometry analysis. Whereas BRI1 ASSOCIATED KINASE1 (BAK1) and several other known BRI1 interactors coimmunoprecipitated with BRL3, no evidence was found of a direct interaction between BRI1 and BRL3. In addition, we confirmed that BAK1 interacts with the BRL1 receptor by coimmunoprecipitation and fluorescence microscopy analysis. Importantly, genetic analysis of brl1 brl3 bak1-3 triple mutants revealed that BAK1, BRL1, and BRL3 signaling modulate root growth and development by contributing to the cellular activities of provascular and quiescent center cells. This provides functional relevance to the observed protein-protein interactions of the BRL3 signalosome. Overall, our study demonstrates that cell-specific BR receptor complexes can be assembled to perform different cellular activities during plant root growth, while highlighting that immunoprecipitation of leucine-rich repeat receptor kinases in plants is a powerful approach for unveiling signaling mechanisms with cellular resolution in plant development.

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Section: Discussionmentioning

confidence: 99%

The BRASSINOSTEROID INSENSITIVE1–LIKE3 Signalosome Complex Regulates Arabidopsis Root Development

Fàbregas

Boeren

et al. 2013

The Plant Cell

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“…Consistent with an involvement of BRs in xylem development, treatment with the BR biosynthetic inhibitor brassinazole (Brz) hampers the development of secondary xylem in cress plants (Lipidium sativum) (Nagata et al, 2001). In addition, BRs modulate the number of vascular bundles by promoting early procambial divisions, whereas periodic auxin maxima control their positioning (Ibañ es et al, 2009;Fà bregas et al, 2010). Among the BR genes involved in vascular tissue development are BRI1 and its close homologs BRI1-LIKE1 (BRL1), BRL2, and BRL3.…”

Section: Plant Architecture and Biomassmentioning

confidence: 99%

Boosting Crop Yields with Plant Steroids

2012

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Plant sterols and steroid hormones, the brassinosteroids (BRs), are compounds that exert a wide range of biological activities. They are essential for plant growth, reproduction, and responses to various abiotic and biotic stresses. Given the importance of sterols and BRs in these processes, engineering their biosynthetic and signaling pathways offers exciting potentials for enhancing crop yield. In this review, we focus on how alterations in components of sterol and BR metabolism and signaling or application of exogenous steroids and steroid inhibitors affect traits of agronomic importance. We also discuss areas for future research and identify the fine-tuning modulation of endogenous BR content as a promising strategy for crop improvement.

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“…To help understand the complex cellular responses to BRI1-mediated BR signaling, a mathematical model is beneficial. Modeling was previously employed to describe the radial patterning of the vascular bundles in shoots, which is controlled by polar auxin transport and BR signaling (Ibañes et al, 2009;Fàbregas et al, 2010), and to help understand the role of the BREVIS RADIX (BRX) gene in BR and auxin signaling (Sankar et al, 2011).…”

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confidence: 99%

A Mathematical Model for BRASSINOSTEROID INSENSITIVE1-Mediated Signaling in Root Growth and Hypocotyl Elongation

Esse¹,

Mourik

Stigter

et al. 2012

Plant Physiology

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(S.v.M., J.M.) Brassinosteroid (BR) signaling is essential for plant growth and development. In Arabidopsis (Arabidopsis thaliana), BRs are perceived by the BRASSINOSTEROID INSENSITIVE1 (BRI1) receptor. Root growth and hypocotyl elongation are convenient downstream physiological outputs of BR signaling. A computational approach was employed to predict root growth solely on the basis of BRI1 receptor activity. The developed mathematical model predicts that during normal root growth, few receptors are occupied with ligand. The model faithfully predicts root growth, as observed in bri1 loss-of-function mutants. For roots, it incorporates one stimulatory and two inhibitory modules, while for hypocotyls, a single inhibitory module is sufficient. Root growth as observed when BRI1 is overexpressed can only be predicted assuming that a decrease occurred in the BRI1 half-maximum response values. Root growth appears highly sensitive to variation in BR concentration and much less to reduction in BRI1 receptor level, suggesting that regulation occurs primarily by ligand availability and biochemical activity.

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A systems biology approach to dissect the contribution of brassinosteroid and Auxin hormones to vascular patterning in the shoot ofArabidopsis thaliana

Abstract: Addendum to: Ibañes M, Fàbregas N, Chory J, Caño-Delgado AI. Brassinosteroid signaling and auxin transport are required to establish the periodic pattern of Arabidopsis shoot vascular bundles.

Cited by 14 publications

References 36 publications

The BRASSINOSTEROID INSENSITIVE1–LIKE3 Signalosome Complex Regulates Arabidopsis Root Development

The BRASSINOSTEROID INSENSITIVE1–LIKE3 Signalosome Complex Regulates Arabidopsis Root Development

Boosting Crop Yields with Plant Steroids

A Mathematical Model for BRASSINOSTEROID INSENSITIVE1-Mediated Signaling in Root Growth and Hypocotyl Elongation

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