A Systems Approach and Skeletal Myogenesis

Ito, Yoshiaki; Kayama, Tomohiro; Asahara, Hiroshi

doi:10.1155/2012/759407

Cited by 12 publications

(9 citation statements)

References 43 publications

(49 reference statements)

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“…MYOD1 and MYF5 are involved in the early phases of skeletal muscle development by promoting the proliferation and differentiation of myogenic progenitor cells into myoblasts, while MYOG plays an important role in the latter phases of myogenesis that involve fusion of myoblasts into myotubes. The precise function of MYF6 remains unknown, though it is thought to regulate myogenesis, and is exclusively expressed in skeletal muscles ( 9 ).…”

Section: Introductionmentioning

confidence: 99%

Screening and Identification of Muscle-Specific Candidate Genes via Mouse Microarray Data Analysis

Raza

Liang²,

Wang³

et al. 2021

Front. Vet. Sci.

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Muscle tissue is involved with every stage of life activities and has roles in biological processes. For example, the blood circulation system needs the heart muscle to transport blood to all parts, and the movement cannot be separated from the participation of skeletal muscle. However, the process of muscle development and the regulatory mechanisms of muscle development are not clear at present. In this study, we used bioinformatics techniques to identify differentially expressed genes specifically expressed in multiple muscle tissues of mice as potential candidate genes for studying the regulatory mechanisms of muscle development. Mouse tissue microarray data from 18 tissue samples was selected from the GEO database for analysis. Muscle tissue as the treatment group, and the other 17 tissues as the control group. Genes expressed in the muscle tissue were different to those in the other 17 tissues and identified 272 differential genes with highly specific expression in muscle tissue, including 260 up-regulated genes and 12 down regulated genes. is the genes were associated with the myofibril, contractile fibers, and sarcomere, cytoskeletal protein binding, and actin binding. KEGG pathway analysis showed that the differentially expressed genes in muscle tissue were mainly concentrated in pathways for AMPK signaling, cGMP PKG signaling calcium signaling, glycolysis, and, arginine and proline metabolism. A PPI protein interaction network was constructed for the selected differential genes, and the MCODE module used for modular analysis. Five modules with Score > 3.0 are selected. Then the Cytoscape software was used to analyze the tissue specificity of differential genes, and the genes with high degree scores collected, and some common genes selected for quantitative PCR verification. The conclusion is that we have screened the differentially expressed gene set specific to mouse muscle to provide potential candidate genes for the study of the important mechanisms of muscle development.

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Section: Introductionmentioning

confidence: 99%

Screening and Identification of Muscle-Specific Candidate Genes via Mouse Microarray Data Analysis

Raza

Liang²,

Wang³

et al. 2021

Front. Vet. Sci.

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show abstract

“…During embryonic myogenesis, mesoderm-derived myoblasts fuse into multinucleate muscle fibers. These myogenic progenitor cells are quiescent and convert into mature skeletal muscles with the ability to function as muscles [ 16 , 17 ]. As mature muscles have the capacity to regenerate after damage, it is critical to establish normal muscles in the embryo stage.…”

Section: Introductionmentioning

confidence: 99%

Loss of splicing factor IK impairs normal skeletal muscle development

Seo

Choi

et al. 2021

BMC Biol

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Background IK is a splicing factor that promotes spliceosome activation and contributes to pre-mRNA splicing. Although the molecular mechanism of IK has been previously reported in vitro, the physiological role of IK has not been fully understood in any animal model. Here, we generate an ik knock-out (KO) zebrafish using the CRISPR/Cas9 system to investigate the physiological roles of IK in vivo. Results The ik KO embryos display severe pleiotropic phenotypes, implying an essential role of IK in embryonic development in vertebrates. RNA-seq analysis reveals downregulation of genes involved in skeletal muscle differentiation in ik KO embryos, and there exist genes having improper pre-mRNA splicing among downregulated genes. The ik KO embryos display impaired neuromuscular junction (NMJ) and fast-twitch muscle development. Depletion of ik reduces myod1 expression and upregulates pax7a, preventing normal fast muscle development in a non-cell-autonomous manner. Moreover, when differentiation is induced in IK-depleted C2C12 myoblasts, myoblasts show a reduced ability to form myotubes. However, inhibition of IK does not influence either muscle cell proliferation or apoptosis in zebrafish and C2C12 cells. Conclusion This study provides that the splicing factor IK contributes to normal skeletal muscle development in vivo and myogenic differentiation in vitro.

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“…Regulation of the activity of myogenic stem cells involves many transcription factors, such as myoblast determination protein 1 (MyoD1), myogenin (MyoG), myogenic factor 5 (Myf5), and myogenic factor 6 (Myf6), as well as other myogenesis-related regulatory transcription factors, including the paired box 3 (PAX3), paired box 7 (PAX7), and myocyte enhancer factor 2 (MEF2) families [6][7][8][9]. Among these, MyoD1 and Myf5 are involved in the rst stage of skeletal muscle development, which promoting the proliferation and differentiation of myogenic progenitor cells into myoblasts, while MyoG determines the generation of myotubes, and Myf6 is involved in cell differentiation and cell fate [10]. Myf5 and MyoD1 are myogenic determinants, as has been demonstrated using double-or triple-knockout (KO) mice [11,12].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of Circular RNA-Mediated ceRNA Regulation in Porcine Skeletal Muscle Development

Yun

Huang

Liu

et al. 2022

Preprint

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Background: As a diverse and abundant class of endogenous RNAs, circular RNAs (circRNAs) participate in various biological processes including cell proliferation and apoptosis. Nevertheless, few researchers have investigated the role of circRNAs in muscle development in cultivated pigs. Results: In this study, we used RNA-seq to construct circRNA expression profiles in skeletal muscle of Jinfen White pigs at the age of 1, 90, and 180 days. Among the 16,990 identified circRNAs, 584 were differentially expressed, with 255, 477, and 63 DE circRNAs in the 90 d vs. 1 d, 180 d vs. 1 d, and 180 d vs. 90 d groups, respectively. Moreover, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of DE circRNA host genes revealed them to be mainly involved in skeletal muscle fiber-related processes (e.g., muscle contraction, muscle organ development, and muscle system processes) and skeletal muscle fiber-related signaling pathways (e.g., AMPK and cAMP pathways). We also constructed circRNA–miRNA–mRNA co-expression network to screen out circRNAs many involved in the regulation of porcine skeletal muscle growth and development through the competitive endogenous RNA (ceRNA) mechanism. In this network, we predicted circ_0018595 may as a potential sponge of miR-1343 to regulate PGM1 expression, in turn promoting the proliferation of pig skeletal muscle satellite cells. The structure and expression of circ_0018595 were confirmed using convergent and divergent primer amplification, RNase R digestion, and qRT-PCR. Conclusions: This study has identified 584 candidate circRNAs, especially circ_0018595, which may be involved in the growth and development of porcine skeletal muscle, and will therefore serve as a valuable resource for further in-depth study of circRNA regulatory mechanisms in skeletal muscle development.

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A Systems Approach and Skeletal Myogenesis

Cited by 12 publications

References 43 publications

Screening and Identification of Muscle-Specific Candidate Genes via Mouse Microarray Data Analysis

Screening and Identification of Muscle-Specific Candidate Genes via Mouse Microarray Data Analysis

Loss of splicing factor IK impairs normal skeletal muscle development

Genome-Wide Analysis of Circular RNA-Mediated ceRNA Regulation in Porcine Skeletal Muscle Development

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