A systematic view of the rice calcineurin B-like protein interacting protein kinase family

Abstract

Calcium (Ca 2? ), as a universal second messenger, plays an important role in various signal transduction pathway networks in plants. There are several components of Ca 2? mediating signaling pathways including calcium-dependent protein kinases, calmodulin and calcineurin B-like proteins (CBLs), and CBL-interacting protein kinases (CIPK). Here, we provide a systematic view of 33 rice CIPK (OsCIPK) family members, which are further classified into seven subgroups. The integration of various meta-expression data such as anatomy, diurnal regulation, and phylogenomic tree context of the OsCIPK family reveals that 11 of the family members show preferred expression in leaves during vegetative growth. In addition, 20 OsCIPK genes show peak expression during the day, while none of the genes peaked expression in the dark. Regarding abiotic stress, 16 OsCIPK genes were induced by drought stress in leaf organs; of these, 14 OsCIPK genes showed up-regulation and two OsCIPK genes were down-regulated based on quantitative RT-PCR analyses. We then developed a functional gene network that consists of 15 out of the 16 genes. This network proposes a useful hypothetical model to understand the molecular mechanism of drought response and circadian regulation associated with OsCIPK family genes.

Identification and characterization of CBL and CIPK gene families in eggplant (Solanum melongena L.)

“…Based on the conservative sequences, structures and similar functions of CBL-CIPK network in plants, we can study the CBLs and CIPKs in other species to complement the signaling pathway. The mechanism by which CBL-CIPK complexes regulate ion homeostasis under salt stress has been well studied in Arabidopsis and rice (Giong et al 2015;Guo et al 2015;Qiu et al 2002;Quintero et al 2002;Kim et al 2007;Kolukisaoglu et al 2004). However, the identities and roles of the CBL-CIPK signaling network in eggplant are still unknown.…”