A Systematic Review on Anti-Inflammatory Activity of Harpagoside

Park, Kyoung Sik

doi:10.17554/j.issn.2313-7177.2016.02.27

Cited by 8 publications

(1 citation statement)

References 40 publications

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“…The two compounds were sparteine sulfate (SS), which is an alkaloid found in Lupinus species and other Leguminosae, and harpagoside (Harp), an iridoid glycoside found in extracts of the plant Harpagophytum procumbens, also known as devil's claw. SS has been shown to be useful as an antiarrhythmic, oxytocic and anticonvulsant [15,22,[24][25][26][27], while Harp has been shown to have analgesic and anti-inflammatory properties [28]. Despite the reported medicinal uses, this is the first study to assess their impact on T cells and their ability to induce Tregs in vitro.…”

Section: Introductionmentioning

confidence: 99%

Induction of CD4+CD25+ Regulatory T Cells from In Vitro Grown Human Mononuclear Cells by Sparteine Sulfate and Harpagoside

Atwany

Hashemi

Jayakumar

et al. 2020

Biology

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Regulatory T cells (Tregs) are key players in the regulation of inflammatory responses. In this study, two natural molecules, namely, sparteine sulfate (SS) and harpagoside (Harp), were investigated for their ability to induce Tregs in human peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from healthy volunteers and grown in the presence or absence of ConA, with TGF-beta, SS or Harp. Expression of the mRNA of FoxP3, TGF-beta, IL-10 and GAPDH was assessed via q-PCR. The expression of Treg markers including CD4, CD25, CD127 and FoxP3 was measured via flow cytometry. The secretion of IL-10 and TGF-beta by cultured cells was assessed by ELISA. Furthermore, the suppressive role of SS and Harp on PBMCs in vitro was tested via allogeneic mixed lymphocyte reaction (MLR). Data obtained show that both compounds increased the expression of FoxP3, TGF-beta and IL-10 mRNA in resting PBMCs but to a lesser extent in activated cells. Moreover, they significantly increased the percent of CD4+CD25+FoxP3+CD127− Tregs in activated and naïve PBMCs. Functionally, both compounds caused a significant reduction in the stimulation index in allogeneic MLR. Together, our data demonstrate for the first time that SS and Harp can induce human Tregs in vitro and therefore have great potential as anti-inflammatory agents.

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