A systematic review of post-harvest interventions for Vibrio parahaemolyticus in raw oysters

Spaur, Maya; Davis, Benjamin J. K.; Kivitz, Scott; DePaola, Angelo; Bowers, John C.; Curriero, Frank C.; Nachman, Keeve E.

doi:10.1016/j.scitotenv.2020.140795

Cited by 20 publications

(27 citation statements)

References 53 publications

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“…Our samples were collected in mid-October 2020 ( Supplementary Table 1 ), and the average temperature in Guangdong region during this period was 23.7°C, which was considered to be relatively suitable for the growth of Vibrios ( Turner et al, 2014 ). In addition, although short-term storage at low temperatures can limit bacterial growth in oysters ( Spaur et al, 2020 ), V. parahaemolyticus will not thoroughly death and can recover their viability in appropriate situation ( Xie et al, 2019 ). This may partially explain the observation that the oysters sold in Guangzhou city still showed high abundance of V. parahaemolyticus and V. vulnificus after a period of storage ( Supplementary Table 2 ).…”

Section: Discussionmentioning

confidence: 99%

Microbial Profiles of Retail Pacific Oysters (Crassostrea gigas) From Guangdong Province, China

Wang

2021

Front. Microbiol.

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Oysters are one of the main aquatic products sold in coastal areas worldwide and are popular among consumers because of their delicious taste and nutritional value. However, the microorganisms present in oysters may pose health risks to consumers. In this study, the microbial communities of Pacific oysters (Crassostrea gigas) collected from aquatic product markets in three cities (Guangzhou, Zhuhai, and Jiangmen) of Guangdong Province, China, where raw oysters are popular, were investigated. The plate counts of viable bacteria in oysters collected in the three cities were all approximately 2 log colony-forming units/g. High-throughput sequencing analysis of the V3–V4 region of the 16Sribosomal DNA gene showed a high level of microbial diversity in oysters, as evidenced by both alpha and beta diversity analysis. Proteobacteria, Bacteroidetes, and Firmicutes were the dominant phyla of the microorganisms present in these samples. A variety of pathogenic bacteria, including the fatal foodborne pathogen Vibrio vulnificus, were found, and Vibrio was the dominant genus. Additionally, the relationship between other microbial species and pathogenic microorganisms may be mostly symbiotic in oysters. These data provide insights into the microbial communities of retail oysters in the Guangdong region and indicate a considerable risk related to the consumption of raw oysters.

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Section: Discussionmentioning

confidence: 99%

Microbial Profiles of Retail Pacific Oysters (Crassostrea gigas) From Guangdong Province, China

Wang

2021

Front. Microbiol.

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“…Thomas (2016) used an onboard icing unit for their boat to immediately cool harvested oysters to slow Vibrio reproduction; however, VV and VP concentrations were not significantly reduced, and oyster mortality was heightened. Another review discusses other PHP techniques for the reduction of VP in oysters (Spaur et al., 2020). While many approved PHP techniques can successfully reduce VP and VV in oysters, almost all result in oyster mortality.…”

Section: Raw Oyster Safety Management Related To Depurationmentioning

confidence: 99%

Depuration of live oysters to reduce Vibrio parahaemolyticus and Vibrio vulnificus: A review of ecology and processing parameters

Campbell

Chouljenko

Hall

2022

Comp Rev Food Sci Food Safe

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Consumption of raw oysters, whether wild-caught or aquacultured, may increase health risks for humans. Vibrio vulnificus and Vibrio parahaemolyticus are two potentially pathogenic bacteria that can be concentrated in oysters during filter feeding. As Vibrio abundance increases in coastal waters worldwide, ingesting raw oysters contaminated with V. vulnificus and V. parahaemolyticus can possibly result in human illness and death in susceptible individuals. Depuration is a postharvest processing method that maintains oyster viability while they filter clean salt water that either continuously flows through a holding tank or is recirculated and replenished periodically. This process can reduce endogenous bacteria, including coliforms, thus providing a safer, live oyster product for human consumption; however, depuration of Vibrios has presented challenges. When considering the difficulty of removing endogenous Vibrios in oysters, a more standardized framework of effective depuration parameters is needed. Understanding Vibrio ecology and its relation to certain depuration parameters could help optimize the process for the reduction of Vibrio. In the past, researchers have manipulated key depuration parameters like depuration processing time, water salinity, water temperature, and water flow rate and explored the use of processing additives to enhance disinfection in oysters. In summation, depuration processing from 4 to 6 days, low temperature, high salinity, and flowing water effectively reduced V. vulnificus and V. parahaemolyticus in live oysters. This review aims to emphasize trends among the results of these past works and provide suggestions for future oyster depuration studies.

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“…In this regard, norovirus and Vibrio spp. infections have gathered scientific community attention due to the pathogenicity and virulence behavior of these etiologic agents 13 – 15 . Although some studies have speculated that the identification of multidrug-resistant (MDR) bacteria in marine bivalves could also represent a potential threat for seafood consumers 12 , 16 , 17 , there is scarce scientific evidence regarding this matter.…”

Section: Introductionmentioning

confidence: 99%

Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves

Bueris

Sellera

Fuga

et al. 2022

Sci Rep

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The global spread of critical-priority antimicrobial-resistant Enterobacterales by food is a public health problem. Wild-caught seafood are broadly consumed worldwide, but exposure to land-based pollution can favor their contamination by clinically relevant antimicrobial-resistant bacteria. As part of the Grand Challenges Explorations: New Approaches to Characterize the Global Burden of Antimicrobial Resistance Program, we performed genomic surveillance and cell culture-based virulence investigation of WHO critical priority Enterobacterales isolated from marine bivalves collected in the Atlantic Coast of South America. Broad-spectrum cephalosporin-resistant Klebsiella pneumoniae and Escherichia coli isolates were recovered from eight distinct geographical locations. These strains harbored blaCTX-M-type or blaCMY-type genes. Most of the surveyed genomes confirmed the convergence of wide virulome and resistome (i.e., antimicrobials, heavy metals, biocides, and pesticides resistance). We identified strains belonging to the international high-risk clones K. pneumoniae ST307 and E. coli ST131 carrying important virulence genes, whereas in vitro experiments confirmed the high virulence potential of these strains. Thermolabile and thermostable toxins were identified in some strains, and all of them were biofilm producers. These data point to an alarming presence of resistance and virulence genes in marine environments, which may favor horizontal gene transfer and the spread of these traits to other bacterial species.

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A systematic review of post-harvest interventions for Vibrio parahaemolyticus in raw oysters

Cited by 20 publications

References 53 publications

Microbial Profiles of Retail Pacific Oysters (Crassostrea gigas) From Guangdong Province, China

Microbial Profiles of Retail Pacific Oysters (Crassostrea gigas) From Guangdong Province, China

Depuration of live oysters to reduce Vibrio parahaemolyticus and Vibrio vulnificus: A review of ecology and processing parameters

Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves

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