A systematic review of genotypes and serogroups of African swine fever virus

Qu, Hailong; Ge, Shengxiang; Zhang, Yongqiang; Wu, Xiaodong; Wang, Zhiliang

doi:10.1007/s11262-021-01879-0

Cited by 56 publications

(61 citation statements)

References 82 publications

(101 reference statements)

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“…Traditionally, genotyping of ASFV strains depended on the phylogenetic analysis of the B646L ( p72 ) and E183L ( p54 ) gene sequences, and the analysis of the E183L ( p54 ) gene sequences could improve the identification of each genotype ( 30 , 32 , 33 , 47 , 48 ). However, some scholars proposed that the B646L ( p72 ) gene could not accurately define the serogroup of ASFV, distinguish viruses with different virulence, or predict the effectiveness of specific ASFV vaccines, so they advocated to determine the serogroup of ASFVs based on the phylogenetic analysis of the EP402R ( CD2v ) gene ( 34 , 49 , 50 ). Therefore, it is recommended that genotyping should be combined with serotyping for epidemiological investigation of ASFV.…”

Section: Discussionmentioning

confidence: 99%

“…The CVR of the B602L gene has been widely used to distinguish closely related ASFVs, but the relatively low CVR genetic variability necessitates further research on alternative and more informative gene regions, so the sequence analysis of the IGR between I73R/I329L genes is used to determine the relationship within the relevant genotypes and origin of circulating ASFV strains ( 28 , 29 , 32 , 34 , 36 , 49 , 52 – 55 ). According to the results in this study, the IGR II and IGR III variants were simultaneously circulating in Guangxi province ( Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

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Molecular Characterization of African Swine Fever Virus From 2019-2020 Outbreaks in Guangxi Province, Southern China

et al. 2022

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African swine fever virus (ASFV) causes contagious hemorrhagic disease of pigs with high morbidity and mortality. To identify the molecular characteristics of ASFV strains circulating in Guangxi province, southern China, a total of 336 tissue samples collected from 336 domestic pigs that died as a result of severe hemorrhagic disease during 2019–2020 were tested for ASFV. Furthermore, 66 ASFV strains were genetically characterized by sequence analysis of the C-terminal region of B646L (p72) gene, the complete E183L (p54) gene, the variable region of EP402R (CD2v) gene, the central variable region (CVR) of B602L gene, the full MGF505-2R gene, and the tandem repeat sequence (TRS) within intergenic region (IGR) between the I73R and I329L (I73R/I329L) genes. Phylogenetic analysis revealed that the ASFV strains from Guangxi province belonged to genotypes I and II based on the B646L (p72) and E183L (p54) genes, and there were eight different tetrameric TRS variants based on the CVR of B602L gene. Phylogenetic analysis of the EP402R (CD2v) gene revealed that these ASFV strains belonged to serogroups 4 and 8. Eight of the 66 strains belonged to genotype I and serogroup 4, and showed deletion of whole MGF505-2R gene. The sequence analysis of the IGR between the I73R/I329L genes showed that IGR II and III variants were co-circulating in Guangxi province. The results indicated that ASFV strains circulating in Guangxi province during 2019–2020 outbreaks showed high genetic diversity, of which genotypes I and II, as well as serogroups 4 and 8, were simultaneously circulating in Guangxi province, and there existed wild-type and naturally gene-deleted strains in the field. This is the first detailed report on the molecular characterization of the ASFV strains circulating in southern China, and serogroup 4 in China.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of African Swine Fever Virus From 2019-2020 Outbreaks in Guangxi Province, Southern China

et al. 2022

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“…According to the 3 ′ -end sequences of the B646L gene that encodes p72, various genotypes of ASFV can be distinguished (12,13). In Africa, at least 24 genotypes of ASF are naturally circulating.…”

Section: Introductionmentioning

confidence: 99%

A duplex fluorescent quantitative PCR assay to distinguish the genotype I and II strains of African swine fever virus in Chinese epidemic strains

Cao

et al. 2022

Front. Vet. Sci.

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African swine fever (ASF) is a highly contagious hemorrhagic disease that affects domestic and wild pigs. A recent study reported that both ASF virus (ASFV) genotypes I and II have invaded farm-raised pigs in China, causing chronic infection and morbidity. To develop a duplex fluorescent quantitative PCR method to distinguish the ASFV genotypes I and II in Chinese epidemic strains, the probes and primers were designed based on the B646L sequences of genotypes I and II listed in the GenBank database. After optimizing the system, a duplex fluorescent quantitative PCR method for simultaneous detection of ASFV genotypes I and II B646L genes was successfully established. This method had no cross-reaction with Porcine circovirus type 2 (PCV2), Pseudorabies virus (PRV), or Porcine Parvovirus (PPV), indicating that it has strong specificity. The sensitivity results indicated that the minimum detection limit of ASFV genotypes I and II B646L was 10 copies/Rxn. The inter- and intra-group coefficients of variation were both <3%, indicating that the method was highly reproducible. Therefore, the established duplex fluorescent quantitative PCR assay is important for the differential detection and epidemiological investigation of ASFV.

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“…Its causative agent, ASFV, to the Asfarviridae family in the nucleocytoplasmic large DNA virus superfamily [24]. In addition, it is divided into 24 genotypes according to its B646L gene sequences [25], of which the genotype II is the main epidemic strain in China [26]. Currently, there are no approved vaccines or antiviral drugs against ASF [7].…”

Section: Discussionmentioning

confidence: 99%

Preparation of Monoclonal Antibodies against the Viral p54 Protein and a Blocking ELISA for Detection of the Antibody against African Swine Fever Virus

Gao

Xia

Bai

et al. 2022

Viruses

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African swine fever virus (ASFV) causes a highly contagious viral disease in domestic and wild pigs, leading to serious economic losses. As there are no vaccines or drugs available, early accurate diagnosis and eradiation of infected animals are the most important measures for ASFV prevention and control. Therefore, improvement of available diagnostic assays and development of novel effective techniques are required. This study is devoted to generating a new detection platform of blocking monoclonal antibody−based enzyme−linked immunosorbent assay (ELISA) against ASFV p54 protein. Seven monoclonal antibodies against recombinant p54 protein were produced and four epitopes were identified. Three blocking ELISAs were developed with 6A5 and 6F9 mAbs labeled with HRP, respectively, of which the 6A5/6F9−based blocking ELISA displayed the best detection performance, with an AUC of 0.986, sensitivity of 98.36% and specificity of 92.36% in ROC analysis. Moreover, it has an excellent agreement at 96.59% (198/205) when compared to the commercial blocking ELISA (kappa value = 0.920). The method also has high repeatability, with CV <10%, and no cross reaction with the serum antibodies against PRV, PRRSV, CSFV, PCV2 or SVA. This indicates that the 6A5/6F9−based blocking ELISA has high accuracy with good sensitivity and specificity, suitable for viral detection, field surveillance and epidemiological studies.

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A systematic review of genotypes and serogroups of African swine fever virus

Cited by 56 publications

References 82 publications

Molecular Characterization of African Swine Fever Virus From 2019-2020 Outbreaks in Guangxi Province, Southern China

Molecular Characterization of African Swine Fever Virus From 2019-2020 Outbreaks in Guangxi Province, Southern China

A duplex fluorescent quantitative PCR assay to distinguish the genotype I and II strains of African swine fever virus in Chinese epidemic strains

Preparation of Monoclonal Antibodies against the Viral p54 Protein and a Blocking ELISA for Detection of the Antibody against African Swine Fever Virus

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