A systematic review of East African-Indian family of Mycobacterium tuberculosis in Brazil

Duarte, Tonya Azevedo; Nery, Joilda Silva; Boéchat, Neio; Pereira, Susan Martins; Simonsen, Vera; Oliveira, Martha Maria de; Gomes, M. Gabriela M.; Penha‐Gonçalves, Carlos; Barreto, Maurício Lima; Barbosa, Theolis

doi:10.1016/j.bjid.2017.01.005

Cited by 16 publications

(13 citation statements)

References 35 publications

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“…Whether their co-occurrence is related has yet to be investigated. Similarly, the mamB S253L mutation related to the CACGCAG motif seems only present in EAI6 strains, and whilst little is known whether these strains are more virulent than other lineage 1 “ancient” strains, they have spread globally and have been associated with recent outbreaks 18 , unlike other lineage 1 strains.…”

Section: Discussionmentioning

confidence: 99%

Methylation in Mycobacterium tuberculosis is lineage specific with associated mutations present globally

Phelan

Sessions

Tientcheu

et al. 2018

Sci Rep

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DNA methylation is an epigenetic modification of the genome involved in regulating crucial cellular processes, including transcription and chromosome stability. Advances in PacBio sequencing technologies can be used to robustly reveal methylation sites. The methylome of the Mycobacterium tuberculosis complex is poorly understood but may be involved in virulence, hypoxic survival and the emergence of drug resistance. In the most extensive study to date, we characterise the methylome across the 4 major lineages of M. tuberculosis and 2 lineages of M. africanum, the leading causes of tuberculosis disease in humans. We reveal lineage-specific methylated motifs and strain-specific mutations that are abundant globally and likely to explain loss of function in the respective methyltransferases. Our work provides a set of sixteen new complete reference genomes for the Mycobacterium tuberculosis complex, including complete lineage 5 genomes. Insights into lineage-specific methylomes will further elucidate underlying biological mechanisms and other important phenotypes of the epi-genome.

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Section: Discussionmentioning

confidence: 99%

Methylation in Mycobacterium tuberculosis is lineage specific with associated mutations present globally

Phelan

Sessions

Tientcheu

et al. 2018

Sci Rep

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“…Again, membership of this lineage could further be confirmed by loss of a methyltransferase mamB attributed to a missense mutation in the Rv2024c gene [26]. Although lineage 1 has global prevalence, it is more common in East Africa and the Indian sub-continent [27]. Karonga is easily accessible from the East African country of Tanzania which has a higher prevalence of this lineage and so the expectation might have been that a higher prevalence of this lineage would have been observed than in Blantyre.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of Mycobacterium tuberculosis clinical isolates in Blantyre, Malawi

Ndhlovu

Kiran

Sloan

et al. 2019

Heliyon

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Despite the high burden of tuberculosis (TB) worldwide, specific factors influencing disease transmission remain elusive. Long term epidemiological studies and in vitro experimental models provide evidence of variable relative fitness of Mycobacterium tuberculosis (Mtb) strains but few such studies are available. Large sequence polymorphisms (LSP) are a robust molecular marker and are feasible as an epidemiological investigative tool. Few Mtb molecular epidemiological studies have been reported in Malawi owing to lack of laboratories with molecular tools. We characterized the genetic diversity of Mtb clinical isolates amongst TB patients in Blantyre, Malawi. We genotyped 64 Mtb clinical isolates using LSP-PCR, assigned specific lineages and confirmed 18 of the isolates using SMRT sequencing. The 64 isolates clustered into 4 lineages (L1-L4) with L4 predominating. There were 10/64 (16%) isolates belonging to L1, 6/64 (9%) belonging to L2, 2/64 (3%) belonging to L3 and 46/64 (72%) belonging to L4. Comparison with a previous study done in Karonga revealed concordance in L1 and L4 but discodance in L2 and L3. The phylogenetic tree constructed, comprised of 3/4 lineages present in Blantyre with 3/18 belonging to L1, 3/18 belonging to L2 and 12/18 belonging to L4. Four Mtb lineages were present in Blantyre with L4 predominating. Larger studies are needed to understand the molecular epidemiology of TB in Blantyre in light of increased bi-directional migration with South Africa.

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“…There are some measures that are not all that affordable like TB biomarker test which is precise and not all that expensive for the TB distinguishing resistant of TB tolerance [ 11 ]. For the most part, TB is overwhelming in South Asia, India, and East Africa [ 12 ]. Danger of TB event likewise related to kind of organ transplantation and lung transplantation is at high hazard.…”

Section: Introductionmentioning

confidence: 99%

Structural Prediction and Mutational Analysis of Rv3906c Gene of Mycobacterium tuberculosis H₃₇Rv to Determine Its Essentiality in Survival

Beg

Shivangi

Thakur

et al. 2018

Advances in Bioinformatics

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The emergence of tuberculosis is at the peak; therefore to station it at its lower level we hereby try bioinformatics approach against Mycobacterium tuberculosis [M. tuberculosis] pathogenesis. Rv3906c is a conserved hypothetical gene of M. tuberculosis and contains many GTP binding protein motif DXXG which demonstrate that this gene might be processed in a GTP binding or in GTP hydrolyzing manner. This gene shows interaction with its adjacent genes as well as pcnA which is a polymerase and localized in the extracellular region and found to be a soluble protein. Rv3906c has binding pockets for calcium atom at various positions which prove that calcium might have some role during the process of this gene. GTP binding protein motif DXXG is present in various positions and calcium binds at this site with a C-score of 0.25. Mutational analysis on this motif shows the large decrease of stability after mutation of aspartate residue with glycine. Stress conditions like pH and temperature also change stability of the protein. A decrease in stability at this position might play a role in inhibition of survival of the pathogen. These computational studies of this gene might be a successful step towards drug development against tuberculosis.

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A systematic review of East African-Indian family of Mycobacterium tuberculosis in Brazil

Abstract: EAI may be endemic at a low frequency in Brazil, with some clades indicating increased fitness with respect to this population.

Cited by 16 publications

References 35 publications

Methylation in Mycobacterium tuberculosis is lineage specific with associated mutations present globally

Methylation in Mycobacterium tuberculosis is lineage specific with associated mutations present globally

Genetic diversity of Mycobacterium tuberculosis clinical isolates in Blantyre, Malawi

Structural Prediction and Mutational Analysis of Rv3906c Gene of Mycobacterium tuberculosis H₃₇Rv to Determine Its Essentiality in Survival

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A systematic review of East African-Indian family of Mycobacterium tuberculosis in Brazil

Abstract: EAI may be endemic at a low frequency in Brazil, with some clades indicating increased fitness with respect to this population.

Cited by 16 publications

References 35 publications

Methylation in Mycobacterium tuberculosis is lineage specific with associated mutations present globally

Methylation in Mycobacterium tuberculosis is lineage specific with associated mutations present globally

Genetic diversity of Mycobacterium tuberculosis clinical isolates in Blantyre, Malawi

Structural Prediction and Mutational Analysis of Rv3906c Gene of Mycobacterium tuberculosis H37Rv to Determine Its Essentiality in Survival

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Structural Prediction and Mutational Analysis of Rv3906c Gene of Mycobacterium tuberculosis H₃₇Rv to Determine Its Essentiality in Survival