A Systematic Review of Bone Marrow Stromal Cells and Periosteum-Derived Cells for Bone Regeneration

Zhu, Jingxian; Xiong, Jiabi; Ji, Wei

doi:10.1089/ten.teb.2022.0115

Cited by 13 publications

(12 citation statements)

References 132 publications

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“…Periosteum-derived cells have recently been shown to contain a unique skeletal stem cell population with distinct functions during endogenous bone repair (Jeffery et al 2022). Our recent meta-analysis revealed that cells from periosteum possess equivalent in vivo bone-forming capacity as bone marrow mesenchymal cells (Zhu et al 2023). However, the jawbone periosteal cells currently are understudied.…”

Section: Discussionmentioning

confidence: 99%

Endochondral Repair of Jawbone Defects Using Periosteal Cell Spheroids

Zhu,

Zhang,

Jin

et al. 2023

J Dent Res

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Recapitulation of the natural healing process is receiving increasing recognition as a strategy to induce robust tissue regeneration. Endochondral ossification has been recognized as an essential reparative approach in natural jawbone defect healing. However, such an approach has been overlooked in the recent development of cell-based therapeutics for jawbone repair. Therefore, this study aimed to explore a bioinspired stem cell–based strategy for jawbone repair by mimicking the mesenchymal condensation of progenitor cells during the early endochondral ossification process. For this purpose, passage 3 of jawbone periosteum-derived cells (jb-PDCs) was cultured in our previously reported nonadherent microwells (200 µm in diameter, 148 µm in depth, and 100 µm space in between) and self-assembled into spheroids with a diameter of 96.4 ± 5.8 µm after 48 h. Compared to monolayer culture, the jb-PDC spheroids showed a significant reduction of stemness marker expression evidenced by flow cytometry. Furthermore, a significant upregulation of chondrogenic transcription factor SOX9 in both gene and protein levels was observed in the jb-PDC spheroids after 48 h of chondrogenic induction. RNA sequencing and Western blotting analysis further suggested that the enhanced SOX9-mediated chondrogenic differentiation in jb-PDC spheroids was attributed to the activation of the p38 MAPK pathway. Impressively, inhibition of p38 kinase activity significantly attenuated chondrogenic differentiation jb-PDC spheroids, evidenced by a significant decline of SOX9 in both gene and protein levels. Strikingly, the jb-PDC spheroids implanted in 6- to 8-wk-old male C57BL/6 mice with critical-size jawbone defects (1.8 mm in diameter) showed an evident contribution to cartilaginous callus formation after 1 wk, evidenced by histological analysis. Furthermore, micro–computed tomography analysis showed that the jb-PDC spheroids significantly accelerated bone healing after 2 wk in the absence of exogenous growth factors. In sum, the presented findings represent the successful development of cell-based therapeutics to reengineer the endochondral bone repair process and illustrate the potential application to improve bone repair and regeneration in the craniofacial skeleton.

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Section: Discussionmentioning

confidence: 99%

Endochondral Repair of Jawbone Defects Using Periosteal Cell Spheroids

Zhu,

Zhang,

Jin

et al. 2023

J Dent Res

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“…Early animal experiments also proved that the transplantation of BMSCs promoted bone tissue regeneration and that the conditioned medium of BMSCs could promote the osteogenic differentiation of BMSCs. 38,39 In exosomes derived from BMSCs, some miRNAs have also been found to regulate osteoblast differentiation and promote bone tissue regeneration. 7,40,41 Therefore, we extracted the rat BMSC-derived exosomes for the subsequent experiments.…”

Section: Characterization and Identification Of Exosomesmentioning

confidence: 99%

Black phosphorus thermosensitive hydrogels loaded with bone marrow mesenchymal stem cell-derived exosomes synergistically promote bone tissue defect repair

Hua

Peng

et al. 2023

J. Mater. Chem. B

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“…Periosteum is a connective tissue membrane that covers the surface of the bone cortex . The periosteum contains a large number of periosteal-derived stem cells (PDSCs) as well as neuropeptides secreted by neuronal cells, , which play an important role in bone integrity, bone shaping, and remodeling. Because the source of autologous periosteum is limited, whereas allogeneic periosteum has a rejection reaction, researchers mainly focus on artificial periosteum.…”

Section: Introductionmentioning

confidence: 99%

Dual Factor-Loaded Artificial Periosteum Accelerates Bone Regeneration

Yao,

Liang,

Zeng

et al. 2024

ACS Biomater. Sci. Eng.

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In the clinic, inactivation of osteosarcoma using microwave ablation would damage the periosteum, resulting in frequent postoperative complications. Therefore, the development of an artificial periosteum is crucial for postoperative healing. In this study, we prepared an artificial periosteum using silk fibroin (SF) loaded with stromal cell-derived factor-1α (SDF-1α) and calcitonin gene-related peptide (CGRP) to accelerate bone remodeling after the microwave ablation of osteosarcoma. The prepared artificial periosteum showed a sustained release of SDF-1α and CGRP after 14 days of immersion. In vitro culture of rat periosteal stem cells (rPDSCs) demonstrated that the artificial periosteum is favorable for cell recruitment, the activity of alkaline phosphatase, and bone-related gene expression. Furthermore, the artificial periosteum improved the tube formation and angiogenesis-related gene expression of human umbilical vein endothelial cells (HUVECs). In an animal study, the periosteum in the femur of a rabbit was inactivated through microwave ablation and then removed. The damaged periosteum was replaced with the as-prepared artificial periosteum and favored bone regeneration. In all, the designed dual-factor-loaded artificial periosteum is a promising strategy to replace the damaged periosteum in the therapy of osteosarcoma for a better bone-rebuilding process.

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A Systematic Review of Bone Marrow Stromal Cells and Periosteum-Derived Cells for Bone Regeneration

Cited by 13 publications

References 132 publications

Endochondral Repair of Jawbone Defects Using Periosteal Cell Spheroids

Endochondral Repair of Jawbone Defects Using Periosteal Cell Spheroids

Black phosphorus thermosensitive hydrogels loaded with bone marrow mesenchymal stem cell-derived exosomes synergistically promote bone tissue defect repair

Dual Factor-Loaded Artificial Periosteum Accelerates Bone Regeneration

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