A systematic review: breast cancer susceptibility genes

Xie, Zhaoxin

doi:10.1051/e3sconf/202021803039

Cited by 2 publications

(5 citation statements)

References 37 publications

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“…The total monomorphic bands in the breast cancer patient of ABCG2, ER-α, miRNA -152 genes were (12,16,8) bands, while in control were (16,0,12) bands, whereas the monomorphic band may be referred to homogenous genomic regions of the PCR-RAPD-PCR profile.…”

Section: Discussionmentioning

confidence: 92%

“…The total polymorphic bands in the BC patient of AB-CG2,ER-α ,mi-RNA 152 genes were (15,18,8 ) respectively, while in control were (14,34,0) bands; The total monomorphic bands in ABCG2,ER-α ,mi-RNA 152 genes of the patients were (12,16,8 ) respectively; while in control were (16, 0, 12) bands; The total unique bands in ABCG2, ER-α, mi-RNA 152 genes were (10, 5, 2) bands in a patient; while in control were (6, 7, 0) bands, as shown in figure (3) and table (3,4,5,6,7,8,9,10,11).…”

Section: Detection Genetic Polymorphism Of Breast Cancer-related Genesmentioning

confidence: 99%

“…The susceptibility genes are considered a critical risk factor for hereditary and sporadic breast cancer. However; The genesis of breast cancer is usually cumulative mutations of different genes 8 . Several genes linked to breast cancer have been found 9 .…”

Section: Introductionmentioning

confidence: 99%

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Identification of Some Breast Cancer Related Genes by RAPD Technique in Maysan Province, Iraq

Allami

Dragh

2022

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Breast cancer is a heterogeneous disease regarding its morphology, invasive behavior, metastatic capacity, hormone receptor expression and clinical outcome. Many risk factors for breast cancer, including genetic factors, account for 25-30% of the incidence. About 15-30% of breast cancer is heritable due to known familiar highly penetrates genes and the others are sporadic; It is worthy to state that this study was the first in the world to include amplified genes as a PCR template to determine the relationship between their polymorphism and breast cancer incidence using, RAPD of amplified genes. The study was designed first to evaluate the association of ABCG2 gene polymorphism beside miRNA-152 and ER-a using the RAPD technique with breast cancer incidence in Maysan province women, and second to use those genes as indicators for breast cancer prediction and diagnosis. The study included 100 patients with breast cancer and 30 control healthy women, and then all samples were amplified by conventional PCR by specific F and R primer for (ABCG2, ER-α, miRNA-152) genes and then the best (20 PCR product) from which was chosen as the template for PCR RAPD PCR technique. The results revealed there are significant differences (P < 0.05) in the unique band of ABCG2 at marker OPAA 11, OPU 15, OPAA 17, significant differences (P < 0.05) in the total band of ER- α at marker OPAA11, significant differences in the polymorphic band of ER- α at marker OPU 15, significant differences in the unique band of ER- α at marker OPAA11, OPU 15, and significant differences (P < 0.05) in the bands that had been size (50-60) bp, (140 - 150) bp, (170-180 ) bp of miRNA-152 at marker OPAA 17, OPD 18 between breast cancer patients and control. Our study proved the relationship between genetic polymorphism of breast cancer-related genes (ABCG2, ER-α, miRNA-152) and a higher incidence of cancer; The current study recommends employing these results for future prediction and diagnosis of breast cancers.

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Section: Discussionmentioning

confidence: 92%

Section: Detection Genetic Polymorphism Of Breast Cancer-related Genesmentioning

confidence: 99%

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Identification of Some Breast Cancer Related Genes by RAPD Technique in Maysan Province, Iraq

Allami

Dragh

2022

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“…The reaction mixture (20 µL) consisted of template PCR product 2.5 µl, 2.5 µL (OPAA11, OPU15, OPAA17, OPD18) RAPD primer, 7.5µl Nuclease free water, and 7.5 µl GO Taq ®G2 Green master mix. The mixture was incubated in the TECHNE prime thermal cycler (with heating lid) programmed for (35)(36)(37)(38)(39)(40) cycles, each one consisting of as following: a denaturation step (1 min at 94 •C), one annealing step (30 sec at gradient 36-39 •C) and an extension step (1 min at 72 •C).…”

Section: Column Puri Cationmentioning

confidence: 99%

“…Many genes are found related to breast cancer (Sun et al, 2017). The susceptible genes are critical risk factors for both hereditary and sporadic breast cancer (Xie., 2020). ABCB1 gene is located on chromosome 7q21.12 (Sheng X et al, 2012;MUTLU İÇDUYGU et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Detection of Breast Cancer Related Gene Polymorphisms By a New Modified PCR-RAPD-PCR Technique

Allami

Dragh

2022

Preprint

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Breast cancer is one of the most aggressive cancers with high estimated mortality worldwide; many studies around the world have involved polymorphisms in drug resistance genes, tumor suppressor genes, estrogen receptor genes, and regulatory genes related to the development and prognosis of breast cancers. It is worth noting that this study was the first to include these genes as PCR templates to determine the relationship between their polymorphisms and breast cancer incidence using RAPD of amplified genes. The study was designed first to evaluate the association of ABCG2, ABCB1 and BRCA1 gene polymorphisms in addition to miRNA-152 and ER-a using the RAPD technique with breast cancer incidence in women in Maysan, Province and then to employ those genes in the future as biomarkers in breast cancer prediction and diagnosis. This study included 100 patients with breast cancer and 30 healthy control women, and then all samples were amplified by conventional PCR with specific F and R primers for ABCG2, ABCB1, BRCA1, ER-α, and miRNA-152 genes. Then, the best PCR product (20) was chosen as the template for the RAPD technique. The results revealed that all RAPD primers showed polymorphisms with higher values in patient samples, and revealed several mutations in patient samples. Our study proved the relationship between genetic polymorphisms of breast cancer related genes and a higher incidence of cancer. The results of the statistical analysis by using Chi-square showed that there were no significant differences in the types of bands of the ABCG2, ABCB1, BRCA1 between the patient sample and the control sample, at the probability level of P >0.05, and significant differences in the types of bands of the ER-α, and mi-RNA 152 between the patient sample and the control sample, at the probability level of P <0.05. The current study recommends employing these results for future prediction and diagnosis of breast cancers.

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A systematic review: breast cancer susceptibility genes

Cited by 2 publications

References 37 publications

Identification of Some Breast Cancer Related Genes by RAPD Technique in Maysan Province, Iraq

Identification of Some Breast Cancer Related Genes by RAPD Technique in Maysan Province, Iraq

Detection of Breast Cancer Related Gene Polymorphisms By a New Modified PCR-RAPD-PCR Technique

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